KCNQ1 (NM_000218) Human Untagged Clone

CAT#: SC300026

KCNQ1 (untagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 1 (KCNQ1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNQ1
• Synonyms: ATFB1; ATFB3; JLNS1; KCNA8; KCNA9; Kv1.9; Kv7.1; KVLQT1; LQT; LQT1; RWS; SQT2; WRS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000218 edited
  GCAGGCCCTCCTCGTTATGGCCGCGGCCTCCTCCCCGCCCAGGGCCGAGAGGAAGCGCTG
  GGGTTGGGGCCGCCTGCCAGGCGCCCGCCGGGGCAGCGCGGGCCTGGCCAAGAAGTGCCC
  CTTCTCGCTGGAGCTGGCGGAGGGCGGCCCGGCGGGCGGCGCGCTCTACGCGCCCATCGC
  GCCCGGCGCCCCAGGTCCCGCGCCCCCTGCGTCCCCGGCCGCGCCCGCCGCGCCCCCAGT
  TGCCTCCGACCTTGGCCCGCGGCCGCCGGTGAGCCTAGACCCGCGCGTCTCCATCTACAG
  CACGCGCCGCCCGGTGTTGGCGCGCACCCACGTCCAGGGCCGCGTCTACAACTTCCTCGA
  GCGTCCCACCGGCTGGAAATGCTTCGTTTACCACTTCGCCGTCTTCCTCATCGTCCTGGT
  CTGCCTCATCTTCAGCGTGCTGTCCACCATCGAGCAGTATGCCGCCCTGGCCACGGGGAC
  TCTCTTCTGGATGGAGATCGTGCTGGTGGTGTTCTTCGGGACGGAGTACGTGGTCCGCCT
  CTGGTCCGCCGGCTGCCGCAGCAAGTACGTGGGCCTCTGGGGGCGGCTGCGCTTTGCCCG
  GAAGCCCATTTCCATCATCGACCTCATCGTGGTCGTGGCCTCCATGGTGGTCCTCTGCGT
  GGGCTCCAAGGGGCAGGTGTTTGCCACGTCGGCCATCAGGGGCATCCGCTTCCTGCAGAT
  CCTGAGGATGCTACACGTCGACCGCCAGGGAGGCACCTGGAGGCTCCTGGGCTCCGTGGT
  CTTCATCCACCGCCAGGAGCTGATAACCACCCTGTACATCGGCTTCCTGGGCCTCATCTT
  CTCCTCGTACTTTGTGTACCTGGCTGAGAAGGACGCGGTGAACGAGTCAGGCCGCGTGGA
  GTTCGGCAGCTACGCAGATGCGCTGTGGTGGGGGGTGGTCACAGTCACCACCATCGGCTA
  TGGGGACAAGGTGCCCCAGACGTGGGTCGGGAAGACCATCGCCTCCTGCTTCTCTGTCTT
  TGCCATCTCCTTCTTTGCGCTCCCAGCGGGGATTCTTGGCTCGGGGTTTGCCCTGAAGGT
  GCAGCAGAAGCAGAGGCAGAAGCACTTCAACCGGCAGATCCCGGCGGCAGCCTCACTCAT
  TCAGACCGCATGGAGGTGCTATGCTGCCGAGAACCCCGACTCCTCCACCTGGAAGATCTA
  CATCCGGAAGGCCCCCCGGAGCCACACTCTGCTGTCACCCAGCCCCAAACCCAAGAAGTC
  TGTGGTGGTAAAGAAAAAAAAGTTCAAGCTGGACAAAGACAATGGGGTGACTCCTGGAGA
  GAAGATGCTCACAGTCCCCCATATCACGTGCGACCCCCCAGAAGAGCGGCGGCTGGACCA
  CTTCTCTGTCGACGGCTATGACAGTTCTGTAAGGAAGAGCCCAACACTGCTGGAAGTGAG
  CATGCCCCATTTCATGAGAACCAACAGCTTCGCCGAGGACCTGGACCTGGAAGGGGAGAC
  TCTGCTGACACCCATCACCCACATCTCACAGCTGCGGGAACACCATCGGGCCACCATTAA
  GGTCATTCGACGCATGCAGTACTTTGTGGCCAAGAAGAAATTCCAGCAAGCGCGGAAGCC
  TTACGATGTGCGGGACGTCATTGAGCAGTACTCGCAGGGCCACCTCAACCTCATGGTGCG
  CATCAAGGAGCTGCAGAGGAGGCTGGACCAGTCCATTGGGAAGCCCTCACTGTTCATCTC
  CGTCTCAGAAAAGAGCAAGGATCGCGGCAGCAACACGATCGGCGCCCGCCTGAACCGAGT
  AGAAGACAAGGTGACGCAGCTGGACCAGAGGCTGGCACTCATCACCGACATGCTTCACCA
  GCTGCTCTCCTTGCACGGTGGCAGCACCCCCGGCAGCGGCGGCCCCCCCAGAGAGGGCGG
  GGCCCACATCACCCAGCCCTGCGGCAGTGGCGGCTCCGTCGACCCTGAGCTCTTCCTGCC
  CAGCAACACCCTGCCCACCTACGAGCAGCTGACCGTGCCCAGGAGGGGCCCCGATGAGGG
  GTCCTGAGGAGGGGATGGGGCTGGGGGATGGGCCTGAGTGAGAGGGGAGGCCAAGAGTGG
  CCCCACCTGGCCCTCTCTGAAGGAGGCCACCTCCTAAAAGGCCCAGAGAGAAGAGCCCCA
  CTCTCAGAGGCCCCAATACCCCATGGACCATGCTGTCTGGCACAGCCTGCACTTGGGGGC
  TCAGCAAGGCCACCTCTTCCTGGCCGGTGTGGGGGCCCCGTCTCAGGTCTGAGTTGTTAC
  CCCAAGCGCCCTGGCCCCCACATGGTGATGTTGACATCACTGGCATGGTGGTTGGGACCC
  AGTGGCAGGGCACAGGGCCTGGCCCATGTATGGCCAGGAAGTAGCACAGGCTGAGTGCAG
  GCCCACCCTGCTTGGCCCAGGGGGCTTCCTGAGGGGAGACAGAGCAACCCCTGGACCCCA
  GCCTCAAATCCAGGACCCTGCCAGGCACAGGCAGGGCAGGACCAGCCCACGCTGACTACA
  GGGCCGCCGGCAATAAAAGCCCAGGAGCCCATTTGGAGGGCCTGGGCCTGGCTCCCTCAC
  TCTCAGGAAATGCTGACCCATGGGCAGGAGACTGTGGAGACTGCTCCTGAGCCCCCAGCT
  TCCAGCAGGAGGGACAGTCTCACCATTTCCCCAGGGCACGTGGTTGAGTGGGGGGAACGC
  CCACTTCCCTGGGTTAGACTGCCAGCTCTTCCTAGCTGGAGAGGAGCCCTGCCTCTCCGC
  CCCTGAGCCCACTGTGCGTGGGGCTCCCGCCTCCAACCCCTCGCCCAGTCCCAGCAGCCA
  GCCAAACACACAGAAGGGGACTGCCACCTCCCCTTGCCAGCTGTTGAGCCGCAGAGAAGT
  GACGGTTCCTACACAGGACAGGGGTTCCTTCTGGGCATTACGTCGCATAGAAATCAATAA
  TTTGTGGTGATTTGGATCTGTGTTTTAATGAGTTTCACGGTGTGATTTTGATTATTAATT
  GTGCAAGCTTTTCCTAATAAACGTGGAGAATCCCAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000218 unedited
  NACGTCAGCATTTGNTATACGACTCACTATAGGGCGGCCGCGCAATTCGCAGGCCCTCCT
  CGTTATGGCCGCGGCCTCCTCCCCGCCCAGGCCGAGAGGAAGCGCTGGGGTTGGGGCCGC
  CTGCCAGGCGCCCGCCGGGGCAGCGCGGGCCTGGCCAAGAAGTGCCCCTTCTCGCTGGAG
  CTGGCGGAGGGCGGCCCGGCGGGCGGCGCGCTCTACGCGCCCATCGCGCCCGGCGCCCCA
  GGTCCCGCGCCCCCTGCGTCCCCGGCCGCGCCCGCCGCGCCCCCAGTTGCCTCCGACCTT
  GGCCCGCGGCCGCCGGTGAGCCTAGACCCGCGCGTCTCCATCTACAGCACGCGCCGCCCG
  GTGTTGGCGCGCACCCACGTCCAGGGCCGCGTCTACAACTTCCTCGAGCGTCCCACCGGC
  TGGAAATGCTTCGTTTACCACTTCGCCGTCTTCCTCATCGTCCTGGTCTGCCTCATCTTC
  AGCGTGCTGTCCACCATCGAGCAGTATGCCGCCCTGGCCACGGGGACTCTCTTCTGGATG
  GAGATCGTGCTGGTGGTGTTCTTCGGGACGGAGTACGTGGTCCGCCTCTGGTCCGCCGGC
  TGCCGCAGCAAGTACGTGGGCCTCTGGGGGCGGCTGCGCTTTGCCCGGAAGCCCATTTCC
  ATCATCGAACTCATCGTGGTCGTGGCCTCCATGGTGGGCCTCTGCGTGGGCTCCAAAGGG
  CAAGTGTTTGCCACGTACGCCATTCAGGGCATCCGCTTTCCTGCAATCCTGAGGATGCTA
  CACCTCGACCGCCAGGGAGGCACCTGGAGGCTCCTGGGCTCCGTGGTCCTTATTCCCCGC
  CAGGAGCTGAAAACCCCCTGTACAACGGCTTCCTGGGCCTCACTTTCTCTCGAACTTGGG
  TACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000218 unedited
  CTCATTGCTNGATGGTCAACTTGCCAGGGCCAGCAGAGCACTGGGGNAGGGTCACAGGGC
  TGCCACCCGGGATCTGTTCAGGAAACAGCTATGATCGCGGCCGCAATCTAGAGTCGAGTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGATTCTCCACGTTTATTAAGAAAA
  GCTTGCACAATTAATAATCAAAATCACACCGTGAAACTCATTAAAACACAGATCCCAATC
  ACCACAAATTATTGATTTCTATGCGACGTAATGCCCAAAAGGAACCCCTGTCCTGTGTAG
  GAAACCGTCACTTTTCTGCGGCTCAACAGCTGGCAAGGGGAGGTGGCAGTCCCCTTCTGT
  GTGTTTGGCTGGCTGCTGGGACTGGGCGAGGGGTTGGAGGCCGGAGCCCCACGCACAGTG
  GGCTCAAGGGCGGAGAGGCAGGGCTCCTCTCCAGCTAGGAAGAGCTGGCAGTCTAACCCA
  GGGAAGTGGGCGTTCCCCCCACTCAACCACGTGCCCTGGGGAAATGGTGAGACTGTCCCT
  CCTGCTGGAAGCTGGGGGCTCAAGAACAATCTCCACAGTCTCCTGCCCATGGGTCAGCAT
  TTCCTGAGAGTGAGGGAGCCAGGCCCAGGCCCTCCAAATGGGCTCCTGGGCTTTTATTGC
  CCGCGGCCCTGTAATCAGCGTGGGCTTGTCCTGCCCTGCCTGTGCCTGGCAGGGTCCTTG
  AATTGAAGCTGGGGTCCAAGGGGTGCTCTGTCTCCCCTCAGGAACCCCCTGGGCAAACAG
  GGTGGGCCTGCCCTCAACCTGGGCTACTTTCTTGGCCTACATGGGCCAGGCCTTGGGCCC
  TGCCCTTGGGTCCAACCACCATGCCCATGATGGTAACATTCACATGTGGGGGGCCAAGGC
  CCTTGGGGTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000218
• Insert Size: 3100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000218.2, NP_000209.2
• RefSeq Size: 3262 bp
• RefSeq ORF: 2031 bp
• Locus ID: 3784
• Cytogenetics: 11p15.5-p15.4
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Protein Pathways: Vibrio cholerae infection
• Gene Summary: 'This gene encodes a voltage-gated potassium channel required for repolarization phase of the cardiac action potential. This protein can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. Mutations in this gene are associated with hereditary long QT syndrome 1 (also known as Romano-Ward syndrome), Jervell and Lange-Nielsen syndrome, and familial atrial fibrillation. This gene exhibits tissue-specific imprinting, with preferential expression from the maternal allele in some tissues, and biallelic expression in others. This gene is located in a region of chromosome 11 amongst other imprinted genes that are associated with Beckwith-Wiedemann syndrome (BWS), and itself has been shown to be disrupted by chromosomal rearrangements in patients with BWS. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).