Cytokeratin 9 (KRT9) (NM_000226) Human Untagged Clone

CAT#: SC300028

KRT9 (untagged)-Human keratin 9 (KRT9)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT9
• Synonyms: CK-9; EPPK; K9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000226, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCTGCAGACAGTTCTCCTCGTCCTACTTGAGCCGCAGCGGCGGGGGTGGCGGGGGCGGCCTGGGCA
  GCGGGGGCAGCATAAGGTCTTCCTACAGCCGCTTCAGCTCCTCAGGGGGCGGTGGAGGAGGGGGCCGATT
  CAGCTCTTCTAGTGGCTATGGTGGGGGAAGCTCTCGTGTCTGTGGGAGGGGAGGCGGTGGCAGTTTTGGC
  TACAGCTACGGCGGAGGATCTGGGGGTGGTTTTAGTGCCAGTAGTTTAGGCGGTGGCTTTGGGGGTGGTT
  CCAGAGGTTTTGGTGGTGCTTCTGGAGGAGGCTATAGTAGTTCTGGGGGTTTTGGAGGTGGCTTTGGTGG
  TGGTTCTGGAGGTGGCTTTGGTGGTGGCTATGGGAGTGGGTTTGGGGGGTTTGGGGGCTTTGGAGGTGGT
  GCTGGAGGAGGTGATGGTGGTATTCTGACTGCTAATGAGAAGAGCACCATGCAGGAACTCAATTCTCGGC
  TGGCCTCTTACTTGGATAAGGTGCAGGCTCTAGAGGAGGCCAACAACGACCTGGAGAATAAGATCCAGGA
  TTGGTACGACAAGAAGGGACCTGCTGCTATCCAGAAGAACTACTCCCCTTATTATAACACTATTGATGAT
  CTCAAGGACCAGATTGTGGACCTGACAGTGGGCAACAACAAAACTCTCCTGGACATTGACAACACTCGCA
  TGACACTGGATGACTTCAGGATAAAGTTTGAGATGGAGCAAAACCTGCGGCAAGGAGTGGATGCTGACAT
  CAATGGCCTGCGGCAGGTGCTGGACAATCTGACCATGGAGAAGTCTGACCTGGAGATGCAGTATGAGACT
  CTGCAGGAGGAGCTGATGGCCCTCAAGAAGAATCATAAGGAGGAGATGAGTCAGCTGACTGGGCAGAACA
  GTGGAGATGTCAATGTGGAGATAAACGTTGCTCCTGGCAAAGATCTCACCAAGACCCTCAATGACATGCG
  TCAGGAGTATGAGCAGCTCATTGCTAAGAACAGAAAGGACATCGAGAATCAATATGAGACTCAGATAACC
  CAGATCGAGCATGAGGTATCCAGTAGTGGTCAGGAGGTGCAGTCCAGTGCCAAGGAGGTGACCCAGCTCC
  GGCACGGTGTCCAGGAGTTGGAGATTGAGCTGCAGTCTCAGCTCAGCAAGAAAGCAGCTCTGGAGAAGAG
  CTTGGAAGACACGAAGAACCGCTACTGTGGCCAGCTGCAGATGATCCAGGAGCAGATCAGTAACTTGGAG
  GCCCAGATCACTGACGTCCGGCAAGAGATCGAGTGCCAGAATCAGGAATACAGCCTTCTGCTCAGCATTA
  AGATGCGGCTGGAGAAGGAAATCGAGACCTACCACAACCTCCTTGAGGGAGGCCAGGAAGACTTTGAATC
  CTCCGGAGCTGGAAAAATTGGCCTTGGAGGTCGAGGAGGAAGTGGAGGCAGTTATGGAAGAGGATCCAGG
  GGAGGAAGTGGAGGCAGCTATGGTGGAGGAGGAAGTGGAGGTGGCTATGGTGGAGGAAGTGGGTCCAGGG
  GAGGAAGTGGAGGCAGCTACGGTGGAGGAAGTGGTTCTGGAGGAGGTAGTGGAGGTGGCTATGGTGGAGG
  AAGTGGAGGTGGCCATAGCGGAGGAAGTGGAGGTGGTCATAGTGGAGGAAGTGGGGGCAACTATGGAGGA
  GGAAGTGGCTCTGGAGGAGGAAGTGGGGGTGGCTATGGTGGAGGAAGTGGGTCCAGGGGAGGAAGTGGAG
  GCAGCCATGGTGGAGGAAGTGGTTTTGGAGGTGAAAGTGGAGGCAGCTACGGAGGCGGTGAAGAAGCGAG
  TGGAAGTGGTGGCGGCTACGGAGGAGGAAGCGGAAAATCATCCCATTCCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000226
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000226.3, NP_000217.2
• RefSeq Size: 2290 bp
• RefSeq ORF: 1872 bp
• Locus ID: 3857
• Cytogenetics: 17q21.2
• Gene Summary: 'This gene encodes the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this gene cause epidermolytic palmoplantar keratoderma. [provided by RefSeq, Jul 2008]'