Collagen XVII (COL17A1) (NM_000494) Human Untagged Clone

CAT#: SC300082

COL17A1 (untagged)-Human collagen, type XVII, alpha 1 (COL17A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL17A1
• Synonyms: BA16H23.2; BP180; BPA-2; BPAG2; EBR2A; ERED; JEB-I; LAD-1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000494 edited
  AACCAGGTGGCTATGGTATGGATGTAACCAAGAAAAACAAACGAGATGGAACTGAAGTCA
  CTGAGAGAATTGTCACTGAAACAGTAACCACAAGACTTACATCCTTACCACCAAAAGGCG
  GGACCAGCAATGGCTATGCTAAAACAGCCTCTCTTGGTGGAGGGAGCCGGCTGGAGAAAC
  AAAGCCTGACTCATGGCAGCAGCGGCTACATAAACTCAACTGGAAGCACACGAGGCCATG
  CCTCCACCTCTAGTTACAGGAGGGCTCACTCACCTGCCTCCACTCTGCCCAACTCCCCAG
  GCTCAACCTTTGAAAGGAAAACTCACGTTACCCGCCATGCGTATGAAGGGAGCTCCAGTG
  GCAACTCTTCTCCGGAGTACCCTCGGAAGGAATTTGCATCTTCTTCAACCAGAGGACGGA
  GTCAAACACGAGAGAGTGAAATTCGAGTTCGACTGCAGAGTGCGTCCCCATCCACCCGAT
  GGACAGAATTGGATGATGTTAAGCGTTTGCTCAAGGGGAGTCGATCGGCAAGTGTGAGCC
  CCACCCGGAATTCCTCCAACACACTCCCCATCCCCAAGAAAGGCACTGTGGAGACCAAAA
  TTGTGACAGCGAGCTCCCAGTCGGTGTCAGGCACCTACGATGCAATGATCCTGGATGCCA
  ACCTTCCCTCCCATGTGTGGTCCTCCACCCTGCCCGCGGGGTCCTCCATGGGGACCTATC
  ACAACAACATGACAACCCAGAGCTCATCCCTCCTCAACACCAATGCCTACTCTGCGGGAT
  CAGTCTTCGGAGTTCCAAACAACATGGCGTCCTGCTCACCCACTTTGCACCCTGGACTCA
  GCACATCCTCCTCAGTGTTTGGCATGCAGAACAATCTGGCCCCCAGCTTGACCACCCTGT
  CCCATGGCACCACCACCACTTCCACAGCATATGGGGTGAAGAAAAACATGCCCCAGAGTC
  CTGCGGCTGTGAACACTGGCGTTTCCACCTCCGCCGCCTGCACCACAAGTGTGCAGAGCG
  ATGACCTTTTGCACAAGGACTGCAAGTTCCTGATCCTAGAGAAAGACAACACACCTGCTA
  AGAAGGAGATGGAGCTGCTCATCATGACCAAGGACAGCGGGAAGGTCTTTACAGCCTCCC
  CTGCCAGCATCGCTGCAACTTCTTTTTCAGAAGACACCCTAAAAAAAGAAAAGCAAGCTG
  CCTACAATGCTGACTCAGGCCTAAAAGCCGAAGCTAATGGAGACCTGAAGACTGTGTCCA
  CAAAGGGCAAGACCACCACTGCAGATATCCACAGCTACAGCAGCAGTGGTGGTGGTGGCA
  GTGGAGGAGGTGGCGGTGTTGGTGGCGCTGGCGGCGGCCCTTGGGGACCAGCGCCAGCCT
  GGTGCCCCTGCGGCTCCTGCTGCAGCTGGTGGAAGTGGCTGCTGGGCCTGCTGCTCACCT
  GGCTGCTACTCCTGGGGCTGCTCTTCGGCCTCATTGCTCTGGCGGAGGAGGTGAGGAAGC
  TGAAGGCGCGTGTGGATGAGCTGGAGAGGATCAGGAGGAGCATACTGCCCTATGGGGACA
  GCATGGATAGAATAGAAAAGGACCGCCTCCAGGGCATGGCACCCGCGGCGGGAGCAGACC
  TGGACAAAATTGGGCTGCACAGTGACAGCCAGGAGGAGCTCTGGATGTTCGTGAGGAAGA
  AGCTAATGATGGAACAGGAAAATGGAAATCTCCGAGGAAGCCCTGGCCCTAAAGGTGACA
  TGGGAAGTCCAGGCCCTAAAGGAGATCGAGGGTTCCCTGGGACTCCAGGTATCCCTGGGC
  CCTTGGGCCACCCAGGTCCACAAGGACCAAAGGGTCAAAAAGGCAGCGTGGGAGATCCTG
  GCATGGAAGGCCCCATGGGCCAGAGAGGGCGAGAAGGCCCCATGGGACCTCGTGGTGAGG
  CAGGGCCTCCTGGATCTGGAGAGAAAGGGGAAAGAGGGGCTGCTGGTGAACCAGGTCCTC
  ATGGCCCACCTGGTGTCCCAGGTTCTGTGGGTCCCAAAGGTTCCAGCGGCTCTCCTGGCC
  CACAGGGCCCTCCAGGTCCTGTAGGTCTCCAAGGGCTCCGAGGTGAAGTAGGACTTCCTG
  GTGTCAAAGGTGACAAAGGACCAGTGGGACCACCAGGACCCAAAGGTGACCAGGGTGAGA
  AAGGACCTCGAGGCCTCACAGGCGAGCCTGGCATGAGAGGTTTGCCTGGTGCTGTTGGTG
  AGCCCGGGGCTAAAGGAGCAATGGGTCCTGCTGGCCCAGACGGACACCAAGGCCCAAGAG
  GTGAACAAGGTCTTACTGGGATGCCTGGAATCCGTGGCCCACCAGGACCTTCTGGAGACC
  CAGGAAAGCCAGGTCTCACAGGACCCCAGGGACCTCAGGGACTTCCCGGTACCCCTGGCC
  GACCAGGAATAAAAGGTGAACCAGGAGCTCCAGGCAAGATCGTGACTTCGGAGGGGTCAT
  CGATGCTCACTGTCCCAGGCCCCCCAGGACCTCCTGGAGCCATGGGACCCCCAGGACCTC
  CAGGTGCCCCAGGCCCTGCCGGCCCAGCTGGTCTCCCAGGACATCAAGAAGTTCTTAATT
  TACAAGGTCCCCCAGGCCCACCCGGCCCACGCGGGCCACCAGGGCCTTCCATTCCAGGCC
  CACCAGGACCCCGAGGCCCACCAGGGGAGGGTTTGCCAGGCCCACCAGGCCCACCAGGAT
  CGTTCCTGTCCAACTCAGAAACCTTCCTCTCCGGCCCCCCAGGCCCACCTGGCCCCCCAG
  GTCCCAAGGGAGACCAAGGTCCCCCAGGCCCCAGAGGACACCAAGGCGAGCAAGGCCTCC
  CAGGTTTCTCAACCTCAGGGTCCAGTTCTTTCGGACTCAACCTTCAGGGACCACCAGGCC
  CACCTGGCCCCCAGGGACCAAAAGGTGACAAAGGTGATCCGGGTGTTCCAGGGGCTCTTG
  GCATTCCTAGTGGTCCTTCTGAAGGGGGATCATCAAGTACCATGTACGTGTCAGGCCCGC
  CAGGGCCCCCTGGGCCCCCTGGGCCTCCGGGCTCTATCAGCAGCTCTGGCCAGGAGATTC
  AGCAGTACATCTCTGAGTACATGCAGAGTGACAGTATTAGATCTTACCTATCCGGAGTTC
  AGGGTCCCCCAGGCCCACCTGGTCCCCCAGGACCTGTCACCACCATCACAGGCGAGACTT
  TCGACTACTCAGAGCTGGCAAGCCACGTTGTGAGCTACTTACGGACTTCGGGGTACGGTG
  TCAGCTTGTTCTCGTCCTCCATCTCTTCTGAAGACATCCTGGCTGTGCTGCAGCGGGATG
  ACGTGCGTCAGTACCTACGTCAGTACTTGATGGGCCCTCGGGGTCCGCCAGGGCCACCAG
  GAGCCAGTGGAGATGGGTCCCTCCTGTCTTTGGACTATGCAGAGCTGAGTAGTCGCATTC
  TCAGCTACATGTCGAGTTCTGGGATCAGCATTGGGCTTCCTGGTCCCCCGGGGCCCCCTG
  GCTTGCCGGGAACCTCCTATGAGGAGCTCCTCTCCTTGCTGCGAGGGTCTGAATTCAGAG
  GCATCGTTGGACCCCCAGGTCCCCCGGGTCCACCAGGGATCCCAGGCAATGTGTGGTCCA
  GCATCAGCGTGGAGGACCTCTCGTCTTACTTACATACTGCCGGCTTGTCATTCATCCCAG
  GCCCTCCAGGACCTCCTGGTCCCCCAGGGCCTCGAGGGCCCCCGGGTGTCTCAGGAGCCC
  TGGCAACCTATGCAGCTGAAAACAGCGACAGCTTCCGGAGCGAGCTGATCAGCTACCTCA
  CAAGTCCTGATGTGCGCAGCTTCATTGTTGGCCCCCCAGGCCCTCCTGGGCCGCAGGGAC
  CCCCTGGGGACAGCCGCCTCCTGTCCACGGATGCCTCCCACAGTCGGGGTAGCAGCTCCT
  CCTCACACAGCTCATCTGTCAGGCGGGGCAGCTCCTACAGCTCTTCCATGAGCACAGGAG
  GAGGTGGTGCAGGCTCCCTGGGTGCAGGCGGTGCCTTTGGTGAAGCTGCAGGAGACAGGG
  GTCCCTATGGCACTGACATCGGCCCAGGCGGAGGCTATGGGGCAGCAGCAGAAGGCGGCA
  TGTATGCTGGCAATGGCGGACTATTGGGAGCTGACTTTGCTGGAGATCTGGATTACAATG
  AGCTGGCTGTGAGGGTGTCAGAGAGCATGCAGCGTCAGGGCCTACTGCAAGGGATGGCCT
  ACACTGTCCAGGGCCCACCAGGCCAGCCTGGGCCACAGGGGCCACCCGGCATCAGCAAGG
  TCTTCTCTGCCTACAGCAACGTGACTGCGGACCTCATGGACTTCTTCCAAACTTATGGAG
  CCATTCAAGGACCCCCTGGGCAAAAAGGAGAGATGGGCACTCCAGGACCCAAAGGTGACA
  GGGGCCCTGCTGGGCCACCAGGTCATCCTGGGCCACCTGGCCCTCGAGGACACAAGGGAG
  AAAAAGGAGACAAAGGTGACCAAGTCTATGCTGGGCGGAGAAGGAGAAGAAGTATTGCTG
  TCAAGCCGTGAGCTAGCCATGGCAGGACAGCTCCTGGACCAGGTCTCATAATGCATGTGG
  CACTTAGGTCCAAGGTCTCCAGAGGGTGAAAGCTGGAGTCTGTCAATGTCCTACTGAGAC
  AGCACAGCCAACCTAGCTAGCAACATTTGTTTTAGTCTGAACAATATATACTTATAGAAT
  TCAGTCAAAGATACACAATCTGAAACAGCTTCATGGGGTGGACTCTAACAGTAGTTGCAA
  TGTTTTAGAATGAGACTTACTTCTCTGCTATCTAGATCTGAACTCCTTGGCTTCTTTACT
  TAGTTCAAGCCCCAGCCTAGGAAAGCCAGTTACATAAAAGTTGGCTCAGGAGTCTTAGAG
  CTTTACCTAAATAGAGCCCAGAAAACGGAGGATGGGGGTGGGGCGCCTTCCTGGAGGTGA
  CACTTGATGGGGGTGTGTTCTGGTTACTGTTCTAAGGCTGTGCCATCAGCTCCTTCCTCC
  CCTGTTCATTCTGCATTCTCTAGTCAGTTGGCTAAGAAGTGACTCTTGCAACTAAAAAAA
  TTAAGAAATTCACTTCCCCTCTAGGAGGTGATGATAGGGTTTCTAATGGTTATATGTATA
  TCACATTCCCATTTGCTTAGAAAGTCTGATTGTAGCTATGATTGTCCGTAGGCCCATACT
  AGAGTTCATGGATATGTTATACTGAACCAGGCCAGAGCAAACAGAAAAAGAAGGTTGAGG
  GCAATGGACAAGGAAGGAATAAAGGGAGAAGAGGGAAAACAGAAAACCTGATGCTGGGGA
  CACAGCATCAGCTCAAGACGTCACCCTCCATTCTGCACTCAGAAAATGGCACTTGGGGGA
  CTGGGCGCAGTTGGTCTTTAACCACTTTTCAATGTCTAAAAACATTTGTTTGTGGTCTAT
  AAGATGAAACATCATTTCAATCGTAAAATTTCCCATTAAAGAAGTTTTTTTTTCTAATAA
  AAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000494 unedited
  GTACAAATTTGTAAACGACTCATATAGGCGGCCGCCAGTGTGAGGNAATCTGCAGAATTC
  GCCCTTAACCAGGTGGCTATGGTATGGATGTAACCAAGNNAAAAACAAACGAGATGGAAC
  TGAAGTCACTGAGAGAATTGTCACTGAAACAGTAACCACAAGACTTACATCCTTACCACC
  AAAAGCGGGACCAGCAATGGCTATGCTAAAACAGCCTCTCTTGGTGGAGGGAGCCGGCTG
  GAGAAACAAAGCCTGACTCATGGCAGCAGCGGCTACATAAACTCAACTGGAAGCACACGA
  GGCCATGCCTCCACCTCTAGTTACAGGAGGGCTCACTCACCTGCCTCCACTCTGCCCAAC
  TCCCCAGGCTCAACCTTTGAAAGGAAAACTCACGTTACCCGCCATGCGTATGAAGGGAGC
  TCCAGTGGCAACTCTTCTCCGGAGTACCCTCGGAAGGAATTTGCATCTTCTTCAACCAGA
  GGACGGAGTCAAACACGAGAGAGTGAAATTCGAGTTCGACTGCAGAGTGCGTCCCCATCC
  ACCCGATGGACAGAATTGGATGATGTTAAGCGTTTGCTCAAGGGGAGTCGATCGGCAAGT
  GTGAGCCCCACCCGGAATTCCTCCAACACACTCCCCATCCCCAAGAAAGGCACTGTGGAG
  ACCAAAATTGTGACAGCGAGCTCCCAGTCNGTGTCAGGCACCTACGATGCAATGATCCTG
  GATGCCAACCTTCCCTCCCATGTGTGGTCCTCCACCCTGCCCGCGGGGTCCTCCATGGGG
  ACCTATCACAACAACTGACAACCCAGAGCTCATCCCTCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_000494 unedited
  AGNAAAGCACTGGGGNAGGGTCACAGGNATGCCACCCGGGATCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATTAGAAAAAAAAA
  CTTCTTTAATGGGAAATTTTACGATTGAAATGATGTTTCATCTTATAGACCACAAACAAA
  TGTTTTTAGACATTGAAAAGTGGTTAAAGACCAACTGCGCCCAGTCCCCCAAGTGCCATT
  TTCTGAGTGCAGAATGGAGGGTGACGTCTTGAGCTGATGCTGTGTCCCCAGCATCAGGTT
  TTCTGTTTTCCCTCTTCTCCCTTTATTCCTTCCTTGTCCATTGCCCTCAACCTTCTTTTT
  CTGTTTGCTCTGGCCTGGTTCAGTATAACATATCCATGAACTCTAGTATGGGCCTACGGA
  CAATCATAGCTACAATCAGACTTTCTAAGCAAATGGGAATGTGATATACATATAACCATT
  AGAAACCCTATCATCACCTCCTAGAGGGGAAGTGAATTTCTTAATTTTTTTAGTTGCAAG
  AGTCACTTCTTAGCCAACTGACTAGAGAATGCAGAATGAACAGGGGAGGAAGGAGCTGAT
  GGCACAGCCTTAGAACAGTAACCAGAACACACCCCCATCAAGTGTCACCTCCAGGAAGGC
  GCCCCACCCCCATCCTCCGTTTTCTGGGCTCTATTTAGGTAAAGCTCTAAGACTCCTGAG
  CCAACTTTTATGTAACTGGCTTTCCTAGGCTGGGGCTTGAACTAAGTAAAGAAGCCAAGG
  AGTTCAGATCTAGATAGCAGAGAAGTAAGTCTCATTCTAAAACATTGCAACTACTGTTAG
  AGTCCACCCATGAAGCTGTTTCAGATT
  
• Restriction Sites: Please inquire
• ACCN: NM_000494
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000494.2, NP_000485.2
• RefSeq Size: 5610 bp
• RefSeq ORF: 4494 bp
• Locus ID: 1308
• Cytogenetics: 10q25.1
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]'