IL4 (NM_000589) Human Untagged Clone

CAT#: SC300104

IL4 (untagged)-Human interleukin 4 (IL4), transcript variant 1


  "NM_000589" in other vectors (6)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol IL4
Synonyms BCGF-1; BCGF1; BSF-1; BSF1; IL-4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000589 edited
TGCATCGTTAGCTTCTCCTGATAAACTAATTGCCTCACATTGTCACTGCAAATCGACACC
TATTAATGGGTCTCACCTCCCAACTGCTTCCCCCTCTGTTCTTCCTGCTAGCATGTGCCG
GCAACTTTGTCCACGGACACAAGTGCGATATCACCTTACAGGAGATCATCAAAACTTTGA
ACAGCCTCACAGAGCAGAAGACTCTGTGCACCGAGTTGACCGTAACAGACATCTTTGCTG
CCTCCAAGAACACAACTGAGAAGGAAACCTTCTGCAGGGCTGCGACTGTGCTCCGGCAGT
TCTACAGCCACCATGAGAAGGACACTCGCTGCCTGGGTGCGACTGCACAGCAGTTCCACA
GGCACAAGCAGCTGATCCGATTCCTGAAACGGCTCGACAGGAACCTCTGGGGCCTGGCGG
GCTTGAATTCCTGTCCTGTGAAGGAAGCCAACCAGAGTACGTTGGAAAACTTCTTGGAAA
GGCTAAAGACGATCATGAGAGAGAAATATTCAAAGTGTTCGAGCTGAA
>OriGene 5' read for NM_000589 unedited
GGCATTTTGTATACGACTCCTATAGGGCGGCCGCGTAATTCGTCGACTGCATCGTTAGCT
TCTCCTGATAAACTAATTGCCTCACATTGTCACTGCAAATCGACACCTATTAATGGGTCT
CACCTCCCAACTGCTTCCCCCTCTGTTCTTCCTGCTAGCATGTGCCGGCAACTTTGTCCA
CGGACACAAGTGCGATATCACCTTACAGGAGATCATCAAAACTTTGAACAGCCTCACAGA
GCAGAAGACTCTGTGCACCGAGTTGACCGTAACAGACATCTTTGCTGCCTCCAAGAACAC
AACTGAGAAGGAAACCTTCTGCAGGGCTGCGACTGTGCTCCGGCAGTTCTACAGCCACCA
TGAGAAGGACACTCGCTGCCTGGGTGCGACTGCACAGCAGTTCCACAGGCACAAGCAGCT
GATCCGATTCCTGAAACGGCTCGACAGGAACCTCTGGGGCCTGGCGGGCTTGAATTCCTG
TCCTGTGAAGGAAGCCAACCAGAGTACGTTGGAAAACTTCTTGGAAAGGCTAAAGACGAT
CATGAGAGAGAAATATTCAAAGTGTTCGAGCTGAAAAGCTTAACTAGCTAGCGGACCGAC
GCGTTGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAAATGATATCCTG
CATCACCATCACCATCACGTTTAAACGGCCGGCCGCGGTCATAGCTGTTTCCTGAACAGA
TCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCAC
TCCAGTGCCCACCAGCCTTGTCCTAATAAATTAAGTTGCATCATTTTGTCTGACTAGTGT
CTTCTATATATATGGGTGAGGGGTTGGTTATGGGAGCAAGGGCAGTTGGGAAGACAACCT
TGTAGGGCTGCGGGTCTATTGGGACAGCTGGAGTGCATGGCACATCTTGGCTCACTGCAT
TCTCCGCCCTTCTGGTTCAGGCGATT
>OriGene 3' read for NM_000589 unedited
TGCTGATGCACTTCAGGGCCGGAGAGGCACTGGGGAGGGGTCCAGGGATGCCCCCGGGAT
CTGTTCAGGAAACAGCTATGACCGCGGCCGGCCGTTTAAACGTGATGGTGATGGTGATGC
AGGATATCATTTGCCAGATCCTCTTCAGAGATGAGTTTCTGCTCGAGCGGCCGCAACGCG
TCGGTCCGCTAGCTAGTTAAGCTTTTCAGCTCGAACACTTTGAATATTTCTCTCTCATGA
TCGTCTTTAGCCTTTCCAAGAAGTTTTCCAACGTACTCTGGTTGGCTTCCTTCACAGGAC
AGGAATTCAAGCCCGCCAGGCCCCAGAGGTTCCTGTCGAGCCGTTTCAGGAATCGGATCA
GCTGCTTGTGCCTGTGGAACTGCTGTGCAGTCGCACCCAGGCAGCGAGTGTCCTTCTCAT
GGTGGCTGTAGAACTGCCGGAGCACAGTCGCAGCCCTGCAGAAGGTTTCCTTCTCAGTTG
TGTTCTTGGAGGCAGCAAAGATGTCTGTTACGGTCAACTCGGTGCACAGAGTCTTCTGCT
CTGTGAGGCTGTTCAAAGTTTTGATGATCTCCTGTAAGGTGATATCGCACTTGTGTCCGT
GGACAAAGTTGCCGGCACATGCTAGCAGGAAGAACAGAGGGGGAAGCAGTTGGGAGGTGA
GACCCATTAATAGGTGTCGATTTGCAGTGACAATGTGAGGCAATTAGTTTATCAGGAGAA
GCTAACGATGCAGTCGACGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCT
GACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCA
TTTGCGTCAACGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCA
AAACAAACTCCCATTGACGTCAATGGGGGTGGAGAACTTGGAAATCCCCGTGGAGTCAAA
Restriction Sites Please inquire     
ACCN NM_000589
Insert Size 500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000589.2, NP_000580.1
RefSeq Size 921 bp
RefSeq ORF 462 bp
Locus ID 3565
Cytogenetics 5q31.1
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, T cell receptor signaling pathway
Gene Summary 'The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020]'
Transcript Variant: This variant (1) encodes the longest isoform (1).

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