iNOS (NOS2) (NM_000625) Human Untagged Clone

CAT#: SC300111

NOS2 (untagged)-Human nitric oxide synthase 2, inducible (NOS2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NOS2
• Synonyms: HEP-NOS; INOS; NOS; NOS2A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000625 edited
  CAGAAGCTCCTGTTTGTCCCGGCTTGAAGCTGTTTGCTGTGTGACCTTGCCCCACAGTGA
  AGAACATCTGAGCTCAAATCCAGATAAGTGACATAAGTGACCTGCTTTGTAAAGCCATAG
  AGATGGCCTGTCCTTGGAAATTTCTGTTCAAGACCAAATTCCACCAGTATGCAATGAATG
  GGGAAAAAGACATCAACAACAATGTGGAGAAAGCCCCCTGTGCCACCTCCAGTCCAGTGA
  CACAGGATGACCTTCAGTATCACAACCTCAGCAAGCAGCAGAATGAGTCCCCGCAGCCCC
  TCGTGGAGACGGGAAAGAAGTCTCCAGAATCTCTGGTCAAGCTGGATGCAACCCCATTGT
  CCTCCCCACGGCATGTGAGGATCAAAAACTGGGGCAGCGGGATGACTTTCCAAGACACAC
  TTCACCATAAGGCCAAAGGGATTTTAACTTGCAGGTCCAAATCTTGCCTGGGGTCCATTA
  TGACTCCCAAAAGTTTGACCAGAGGACCCAGGGACAAGCCTACCCCTCCAGATGAGCTTC
  TACCTCAAGCTATCGAATTTGTCAACAAATATTACGGCTCCTTCAAAGAGGCAAAAATAG
  AGGAACATCTGGCCAGGGTGGAAGCGGTAACAAAGGAGATAGAAACAACAGGAACCTACC
  AACTGACGGGAGATGAGCTCATCTTCGCCACCAAGCAGGCCTGGCGCAATGCCCCACGCT
  GCATTGGGAGGATCCAGTGGTCCAACCTGCAGGTCTTCGATGCCCGCAGCTGTTCCACTG
  CCCGGGAAATGTTTGAACACATCTGCAGACACGTGCGTTACTCCACCAACAATGGCAACA
  TCAGGTCGGCCATCACCGTGTTCCCCCAGCGGAGTGATGGCAAGCACGACTTCCGGGTGT
  GGAATGCTCAGCTCATCCGCTATGCTGGCTACCAGATGCCAGATGGCAGCATCAGAGGGG
  ACCCTGCCAACGTGGAATTCACTCAGCTGTGCATCGACCTGGGCTGGAAGCCCAAGTACG
  GCCGCTTCGATGTGGTCCCCCTGGTCCTGCAGGCCAATGGCCGTGACCCTGAGCTCTTCG
  AAATCCCACCTGACCTTGTGCTTGAGGTGGCCATGGAACATCCCAAATACGAGTGGTTTC
  GGGAACTGGAGCTAAAGTGGTACGCCCTGCCTGCAGTGGCCAACATGCTGCTTGAGGTGG
  GCGGCCTGGAGTTCCCAGGGTGCCCCTTCAATGGCTGGTACATGGGCACAGAGATCGGAG
  TCCGGGACTTCTGTGACGTCCAGCGCTACAACATCCTGGAGGAAGTGGGCAGGAGAATGG
  GCCTGGAAACGCACAAGCTGGCCTCGCTCTGGAAAGACCAGGCTGTCGTTGAGATCAACA
  TTGCTGTGCTCCATAGTTTCCAGAAGCAGAATGTGACCATCATGGACCACCACTCGGCTG
  CAGAATCCTTCATGAAGTACATGCAGAATGAATACCGGTCCCGTGGGGGCTGCCCGGCAG
  ACTGGATTTGGCTGGTCCCTCCCATGTCTGGGAGCATCACCCCCGTGTTTCACCAGGAGA
  TGCTGAACTACGTCCTGTCCCCTTTCTACTACTATCAGGTAGAGGCCTGGAAAACCCATG
  TCTGGCAGGACGAGAAGCGGAGACCCAAGAGAAGAGAGATTCCATTGAAAGTCTTGGTCA
  AAGCTGTGCTCTTTGCCTGTATGCTGATGCGCAAGACAATGGCGTCCCGAGTCAGAGTCA
  CCATCCTCTTTGCGACAGAGACAGGAAAATCAGAGGCGCTGGCCTGGGACCTGGGGGCCT
  TATTCAGCTGTGCCTTCAACCCCAAGGTTGTCTGCATGGATAAGTACAGGCTGAGCTGCC
  TGGAGGAGGAACGGCTGCTGTTGGTGGTGACCAGTACGTTTGGCAATGGAGACTGCCCTG
  GCAATGGAGAGAAACTGAAGAAATCGCTCTTCATGCTGAAAGAGCTCAACAACAAATTCA
  GGTACGCTGTGTTTGGCCTCGGCTCCAGCATGTACCCTCGGTTCTGCGCCTTTGCTCATG
  ACATTGATCAGAAGCTGTCCCACCTGGGGGCCTCTCAGCTCACCCCGATGGGAGAAGGGG
  ATGAGCTCAGTGGGCAGGAGGACGCCTTCCGCAGCTGGGCCGTGCAAACCTTCAAGGCAG
  CCTGTGAGACGTTTGATGTCCGAGGCAAACAGCACATTCAGATCCCCAAGCTCTACACCT
  CCAATGTGACCTGGGACCCGCACCACTACAGGCTCGTGCAGGACTCACAGCCTTTGGACC
  TCAGCAAAGCCCTCAGCAGCATGCATGCCAAGAACGTGTTCACCATGAGGCTCAAATCTC
  GGCAGAATCTACAAAGTCCGACATCCAGCCGTGCCACCATCCTGGTGGAACTCTCCTGTG
  AGGATGGCCAAGGCCTGAACTACCTGCCGGGGGAGCACCTTGGGGTTTGCCCAGGCAACC
  AGCCGGCCCTGGTCCAAGGTATCCTGGAGCGAGTGGTGGATGGCCCCACACCCCACCAGA
  CAGTGCGCCTGGAGGCCCTGGATGAGAGTGGCAGCTACTGGGTCAGTGACAAGAGGCTGC
  CCCCCTGCTCACTCAGCCAGGCCCTCACCTACTTCCTGGACATCACCACACCCCCAACCC
  AGCTGCTGCTCCAAAAGCTGGCCCAGGTGGCCACAGAAGAGCCTGAGAGACAGAGGCTGG
  AGGCCCTGTGCCAGCCCTCAGAGTACAGCAAGTGGAAGTTCACCAACAGCCCCACATTCC
  TGGAGGTGCTAGAGGAGTTCCCGTCCCTGCGGGTGTCTGCTGGCTTCCTGCTTTCCCAGC
  TCCCCATTCTGAAGCCCAGGTTCTACTCCATCAGCTCCTCCCGGGATCACATGCCCACAG
  AGATCCACCTGACTGTGGCCGTGGTCACCTACCACACCCGAGATGGCCAGGGTCCCCTGC
  ACCACGGCGTCTGCAGCACATGGCTCAACAGCCTGAAGCCCCAAGACCCAGTGCCCTGCT
  TTGTGCGGAATGCCAGCGGCTTCCACCTCCCCGAGGATCCCTCCCATCCTTGCATCCTCA
  TCGGGCCTGGCACAGGCATCGCGCCCTTCCGCAGTTTCTGGCAGCAACGGCTCCATGACT
  CCCAGCACAAGGGAGTGCGGGGAGGCCGCATGACCTTGGTGTTTGGGTGCCGCCGCCCAG
  ATGAGGACCACATCTACCAGGAGGAGATGCTGGAGATGGCCCAGAAGGGGGTGCTGCATG
  CGGTGCACACAGCCTATTCCCGCCTGCCTGGCAAGCCCAAGGTCTATGTTCAGGACATCC
  TGCGGCAGCAGCTGGCCAGCGAGGTGCTCCGTGTGCTCCACAAGGAGCCAGGCCACCTCT
  ATGTTTGCGGGGATGTGCGCATGGCCCGGGACGTGGCCCACACCCTGAAGCAGCTGGTGG
  CTGCCAAGCTGAAATTGAATGAGGAGCAGGTCGAGGACTATTTCTTTCAGCTCAAGAGCC
  AGAAGCGCTATCACGAAGATATCTTTGGTGCTGTATTTCCTTACGAGGCGAAGAAGGACA
  GGGTGGCGGTGCAGCCCAGCAGCCTGGAGATGTCAGCGCTCTGAGGGCCTACAGGAGGGG
  TTAAAGCTGCCGGCACAGAACTTAAGGATGGAGCCAGCTCTGCATTATCTGAGGTCACAG
  GGCCTGGGGAGATGGAGGAAAGTGATATCCCCCAGCCTCAAGTCTTATTTCCTCAACGTT
  GCTCCCCATCAAGCCCTTTACTTGACCTCCTAACAAGTAGCACCCTGGATTGATCGGAGC
  CTCCTCTCTCAAACTGGGGCCTCCCTGGTCCCTTGGAGACAAAATCTTAAATGCCAGGCC
  TGGCAAGTGGGTGAAAGATGGAACTTGCTGCTGAGTGCACCACTTCAAGTGACCACCAGG
  AGGTGCTATCGCACCACTGTGTATTTAACTGCCTTGTGTACAGTTATTTATGCCTCTGTA
  TTTAAAAAACTAACACCCAGTCTGTTCCCCATGGCCACTTGGGTCTTCCCTGTATGATTC
  CTTGATGGAGATATTTACATGAATTGCATTTTACTTTAATCACAAAAAAAAAAAAAAAAA
  A
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000625 unedited
  GGGGAGGATCTGCCATTTTGTATACNACTCACTATTAGGGCGGCCGCGTAATTCGCACGA
  CANAACTCCTGTTTGTCCCGGCTTGAAGCTGTTTGCTGTGTGACCTTGCCCCACAGTGAA
  GAACATCTGAGCTCAAATCCAGATAAGTGACATAAGTGACCTGCTTTGTAAAGCCATAGA
  GATGGCCTGTCCTTGGAAATTTCTGTTCAAGACCAAATTCCACCAGTATGCAATGAATGG
  GGAAAAAGACATCAACAACAATGTGGAGAAAGCCCCCTGTGCCACCTCCAGTCCAGTGAC
  ACAGGATGACCTTCAGTATCACAACCTCAGCAAGCAGCAGAATGAGTCCCCGCAGCCCCT
  CGTGGAGACGGGAAAGAAGTCTCCAGAATCTCTGGTCAAGCTGGATGCAACCCCATTGTC
  CTCCCCACGGCATGTGAGGATCAAAAACTGGGGCAGCGGGATGACTTTCCAAGACACACT
  TCACCATAAGGCCAAAGGGATTTTAACTTGCAGGTCCAAATCTTGCCTGGGGTCCATTAT
  GACTCCCAAAAGTTTGACCAGAGGACCCAGGGACAAGCCTACNCCTCCAGATGAGCTTCT
  ACCTCAAGCTATCGAATTTGTCAACAAATATTACGGCTCCTTCAAAGAGGCAAAAATAGA
  GGAACATCTGGCCCAGGGTGGAAAGCGTAACCAAGGAGATAGAAACAACGGAACCTACCC
  ACTGACGGGAGATGAGCTCATCTTCGCCACCAAGCAGGCCTGGCGCAATGCCCCACGCTG
  CATTGGGAGGATCCAGTGGGTCCACCCTGCAGTCTCCGATGCCCCAGCTGTTCCACTGTC
  CGGGAAATGTTTGAACCCTTTGCCGACACTGCGTTACTCCCCAACCATGGCAACTTCAGT
  CCGGCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000625 unedited
  NGCCCCCATTGGGNNGATGGCAACTTNCCAGGTCCAGNAAAGCACTGGGGNAGGGTCACA
  GGNATGCCACCCGGGCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCG
  AGTTTTTTTTTTTTTTTTTTGTGATTAAAGTAAAATGCAATTCATGTAAATATCTCCATC
  AAGGAATCATACAGGGGAAGACCCAAGTGGCCATGGGGAACAGACTGGGTGTTAGTTTTT
  TAAATACAGAGGCATAAATAACTGTACACAAGGCAGTTAAATACACAGTGGTGCGATAGC
  ACCTCCTGGTGGTCACTTGAAGTGGTGCACTCAGCAGCAAGTTCCATCTTTCACCCACTT
  GCCAGGCCTGGCATTTAAGATTTTGTCTCCAAGGGACCAGGGAGGCCCCAGTTTGAGAGA
  GGAGGCTCCGATCAATCCAGGGTGCTACTTGTTAGGAGGTCAAGTAAAGGGCTTGATGGG
  GAGCAACGTTGAGGAAATAAGACTTGAGGCTGGGGGATATCACTTTCCTCCATCTCCCCA
  GGCCCTGTGACCTCAGATAATGCAGAGCTGGCTCCATCCTTAAGTTCTGTGCCCGCAGCT
  TTAACCCCTCCTGTANGCCCTCAGAGCGCTGACATCTCCAGGCTGCTGGGCTGCACCGNC
  ACCCTGTCCTTCTTCGCCTCGTAAGGAAATACAGCACCAAAGATATCTTCGTGATAGCGC
  TCCTGGCTCTTGGACTGAAAGAAATAGTCCTCGACCTGCTCCTCATTCATTTCAGCTTGG
  CAGCCACCAGCTGCTTCAGGGGTGTGGGCCACGTTCCGGGCCATGCCCACCATCCCGGAA
  AACATAGAAGTTGCCTGGCTTCTTGTGGAACACAAGGACCACTTGCTTGGCCACTGCTGC
  CGAAGGAAGTCCTGAAACTAAACCTGGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000625
• Insert Size: 4100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There is 2 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 2 aa.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000625.3, NP_000616.3
• RefSeq Size: 4221 bp
• RefSeq ORF: 3462 bp
• Locus ID: 4843
• Cytogenetics: 17q11.2
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer
• Gene Summary: 'Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]'