iNOS (NOS2) (NM_000625) Human Untagged Clone

CAT#: SC300111

NOS2 (untagged)-Human nitric oxide synthase 2, inducible (NOS2)


  "NM_000625" in other vectors (6)

Reconstitution Protocol

USD 1,100.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NOS2
Synonyms HEP-NOS; INOS; NOS; NOS2A
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000625 edited
CAGAAGCTCCTGTTTGTCCCGGCTTGAAGCTGTTTGCTGTGTGACCTTGCCCCACAGTGA
AGAACATCTGAGCTCAAATCCAGATAAGTGACATAAGTGACCTGCTTTGTAAAGCCATAG
AGATGGCCTGTCCTTGGAAATTTCTGTTCAAGACCAAATTCCACCAGTATGCAATGAATG
GGGAAAAAGACATCAACAACAATGTGGAGAAAGCCCCCTGTGCCACCTCCAGTCCAGTGA
CACAGGATGACCTTCAGTATCACAACCTCAGCAAGCAGCAGAATGAGTCCCCGCAGCCCC
TCGTGGAGACGGGAAAGAAGTCTCCAGAATCTCTGGTCAAGCTGGATGCAACCCCATTGT
CCTCCCCACGGCATGTGAGGATCAAAAACTGGGGCAGCGGGATGACTTTCCAAGACACAC
TTCACCATAAGGCCAAAGGGATTTTAACTTGCAGGTCCAAATCTTGCCTGGGGTCCATTA
TGACTCCCAAAAGTTTGACCAGAGGACCCAGGGACAAGCCTACCCCTCCAGATGAGCTTC
TACCTCAAGCTATCGAATTTGTCAACAAATATTACGGCTCCTTCAAAGAGGCAAAAATAG
AGGAACATCTGGCCAGGGTGGAAGCGGTAACAAAGGAGATAGAAACAACAGGAACCTACC
AACTGACGGGAGATGAGCTCATCTTCGCCACCAAGCAGGCCTGGCGCAATGCCCCACGCT
GCATTGGGAGGATCCAGTGGTCCAACCTGCAGGTCTTCGATGCCCGCAGCTGTTCCACTG
CCCGGGAAATGTTTGAACACATCTGCAGACACGTGCGTTACTCCACCAACAATGGCAACA
TCAGGTCGGCCATCACCGTGTTCCCCCAGCGGAGTGATGGCAAGCACGACTTCCGGGTGT
GGAATGCTCAGCTCATCCGCTATGCTGGCTACCAGATGCCAGATGGCAGCATCAGAGGGG
ACCCTGCCAACGTGGAATTCACTCAGCTGTGCATCGACCTGGGCTGGAAGCCCAAGTACG
GCCGCTTCGATGTGGTCCCCCTGGTCCTGCAGGCCAATGGCCGTGACCCTGAGCTCTTCG
AAATCCCACCTGACCTTGTGCTTGAGGTGGCCATGGAACATCCCAAATACGAGTGGTTTC
GGGAACTGGAGCTAAAGTGGTACGCCCTGCCTGCAGTGGCCAACATGCTGCTTGAGGTGG
GCGGCCTGGAGTTCCCAGGGTGCCCCTTCAATGGCTGGTACATGGGCACAGAGATCGGAG
TCCGGGACTTCTGTGACGTCCAGCGCTACAACATCCTGGAGGAAGTGGGCAGGAGAATGG
GCCTGGAAACGCACAAGCTGGCCTCGCTCTGGAAAGACCAGGCTGTCGTTGAGATCAACA
TTGCTGTGCTCCATAGTTTCCAGAAGCAGAATGTGACCATCATGGACCACCACTCGGCTG
CAGAATCCTTCATGAAGTACATGCAGAATGAATACCGGTCCCGTGGGGGCTGCCCGGCAG
ACTGGATTTGGCTGGTCCCTCCCATGTCTGGGAGCATCACCCCCGTGTTTCACCAGGAGA
TGCTGAACTACGTCCTGTCCCCTTTCTACTACTATCAGGTAGAGGCCTGGAAAACCCATG
TCTGGCAGGACGAGAAGCGGAGACCCAAGAGAAGAGAGATTCCATTGAAAGTCTTGGTCA
AAGCTGTGCTCTTTGCCTGTATGCTGATGCGCAAGACAATGGCGTCCCGAGTCAGAGTCA
CCATCCTCTTTGCGACAGAGACAGGAAAATCAGAGGCGCTGGCCTGGGACCTGGGGGCCT
TATTCAGCTGTGCCTTCAACCCCAAGGTTGTCTGCATGGATAAGTACAGGCTGAGCTGCC
TGGAGGAGGAACGGCTGCTGTTGGTGGTGACCAGTACGTTTGGCAATGGAGACTGCCCTG
GCAATGGAGAGAAACTGAAGAAATCGCTCTTCATGCTGAAAGAGCTCAACAACAAATTCA
GGTACGCTGTGTTTGGCCTCGGCTCCAGCATGTACCCTCGGTTCTGCGCCTTTGCTCATG
ACATTGATCAGAAGCTGTCCCACCTGGGGGCCTCTCAGCTCACCCCGATGGGAGAAGGGG
ATGAGCTCAGTGGGCAGGAGGACGCCTTCCGCAGCTGGGCCGTGCAAACCTTCAAGGCAG
CCTGTGAGACGTTTGATGTCCGAGGCAAACAGCACATTCAGATCCCCAAGCTCTACACCT
CCAATGTGACCTGGGACCCGCACCACTACAGGCTCGTGCAGGACTCACAGCCTTTGGACC
TCAGCAAAGCCCTCAGCAGCATGCATGCCAAGAACGTGTTCACCATGAGGCTCAAATCTC
GGCAGAATCTACAAAGTCCGACATCCAGCCGTGCCACCATCCTGGTGGAACTCTCCTGTG
AGGATGGCCAAGGCCTGAACTACCTGCCGGGGGAGCACCTTGGGGTTTGCCCAGGCAACC
AGCCGGCCCTGGTCCAAGGTATCCTGGAGCGAGTGGTGGATGGCCCCACACCCCACCAGA
CAGTGCGCCTGGAGGCCCTGGATGAGAGTGGCAGCTACTGGGTCAGTGACAAGAGGCTGC
CCCCCTGCTCACTCAGCCAGGCCCTCACCTACTTCCTGGACATCACCACACCCCCAACCC
AGCTGCTGCTCCAAAAGCTGGCCCAGGTGGCCACAGAAGAGCCTGAGAGACAGAGGCTGG
AGGCCCTGTGCCAGCCCTCAGAGTACAGCAAGTGGAAGTTCACCAACAGCCCCACATTCC
TGGAGGTGCTAGAGGAGTTCCCGTCCCTGCGGGTGTCTGCTGGCTTCCTGCTTTCCCAGC
TCCCCATTCTGAAGCCCAGGTTCTACTCCATCAGCTCCTCCCGGGATCACATGCCCACAG
AGATCCACCTGACTGTGGCCGTGGTCACCTACCACACCCGAGATGGCCAGGGTCCCCTGC
ACCACGGCGTCTGCAGCACATGGCTCAACAGCCTGAAGCCCCAAGACCCAGTGCCCTGCT
TTGTGCGGAATGCCAGCGGCTTCCACCTCCCCGAGGATCCCTCCCATCCTTGCATCCTCA
TCGGGCCTGGCACAGGCATCGCGCCCTTCCGCAGTTTCTGGCAGCAACGGCTCCATGACT
CCCAGCACAAGGGAGTGCGGGGAGGCCGCATGACCTTGGTGTTTGGGTGCCGCCGCCCAG
ATGAGGACCACATCTACCAGGAGGAGATGCTGGAGATGGCCCAGAAGGGGGTGCTGCATG
CGGTGCACACAGCCTATTCCCGCCTGCCTGGCAAGCCCAAGGTCTATGTTCAGGACATCC
TGCGGCAGCAGCTGGCCAGCGAGGTGCTCCGTGTGCTCCACAAGGAGCCAGGCCACCTCT
ATGTTTGCGGGGATGTGCGCATGGCCCGGGACGTGGCCCACACCCTGAAGCAGCTGGTGG
CTGCCAAGCTGAAATTGAATGAGGAGCAGGTCGAGGACTATTTCTTTCAGCTCAAGAGCC
AGAAGCGCTATCACGAAGATATCTTTGGTGCTGTATTTCCTTACGAGGCGAAGAAGGACA
GGGTGGCGGTGCAGCCCAGCAGCCTGGAGATGTCAGCGCTCTGAGGGCCTACAGGAGGGG
TTAAAGCTGCCGGCACAGAACTTAAGGATGGAGCCAGCTCTGCATTATCTGAGGTCACAG
GGCCTGGGGAGATGGAGGAAAGTGATATCCCCCAGCCTCAAGTCTTATTTCCTCAACGTT
GCTCCCCATCAAGCCCTTTACTTGACCTCCTAACAAGTAGCACCCTGGATTGATCGGAGC
CTCCTCTCTCAAACTGGGGCCTCCCTGGTCCCTTGGAGACAAAATCTTAAATGCCAGGCC
TGGCAAGTGGGTGAAAGATGGAACTTGCTGCTGAGTGCACCACTTCAAGTGACCACCAGG
AGGTGCTATCGCACCACTGTGTATTTAACTGCCTTGTGTACAGTTATTTATGCCTCTGTA
TTTAAAAAACTAACACCCAGTCTGTTCCCCATGGCCACTTGGGTCTTCCCTGTATGATTC
CTTGATGGAGATATTTACATGAATTGCATTTTACTTTAATCACAAAAAAAAAAAAAAAAA
A
>OriGene 5' read for NM_000625 unedited
GGGGAGGATCTGCCATTTTGTATACNACTCACTATTAGGGCGGCCGCGTAATTCGCACGA
CANAACTCCTGTTTGTCCCGGCTTGAAGCTGTTTGCTGTGTGACCTTGCCCCACAGTGAA
GAACATCTGAGCTCAAATCCAGATAAGTGACATAAGTGACCTGCTTTGTAAAGCCATAGA
GATGGCCTGTCCTTGGAAATTTCTGTTCAAGACCAAATTCCACCAGTATGCAATGAATGG
GGAAAAAGACATCAACAACAATGTGGAGAAAGCCCCCTGTGCCACCTCCAGTCCAGTGAC
ACAGGATGACCTTCAGTATCACAACCTCAGCAAGCAGCAGAATGAGTCCCCGCAGCCCCT
CGTGGAGACGGGAAAGAAGTCTCCAGAATCTCTGGTCAAGCTGGATGCAACCCCATTGTC
CTCCCCACGGCATGTGAGGATCAAAAACTGGGGCAGCGGGATGACTTTCCAAGACACACT
TCACCATAAGGCCAAAGGGATTTTAACTTGCAGGTCCAAATCTTGCCTGGGGTCCATTAT
GACTCCCAAAAGTTTGACCAGAGGACCCAGGGACAAGCCTACNCCTCCAGATGAGCTTCT
ACCTCAAGCTATCGAATTTGTCAACAAATATTACGGCTCCTTCAAAGAGGCAAAAATAGA
GGAACATCTGGCCCAGGGTGGAAAGCGTAACCAAGGAGATAGAAACAACGGAACCTACCC
ACTGACGGGAGATGAGCTCATCTTCGCCACCAAGCAGGCCTGGCGCAATGCCCCACGCTG
CATTGGGAGGATCCAGTGGGTCCACCCTGCAGTCTCCGATGCCCCAGCTGTTCCACTGTC
CGGGAAATGTTTGAACCCTTTGCCGACACTGCGTTACTCCCCAACCATGGCAACTTCAGT
CCGGCCTT
>OriGene 3' read for NM_000625 unedited
NGCCCCCATTGGGNNGATGGCAACTTNCCAGGTCCAGNAAAGCACTGGGGNAGGGTCACA
GGNATGCCACCCGGGCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCG
AGTTTTTTTTTTTTTTTTTTGTGATTAAAGTAAAATGCAATTCATGTAAATATCTCCATC
AAGGAATCATACAGGGGAAGACCCAAGTGGCCATGGGGAACAGACTGGGTGTTAGTTTTT
TAAATACAGAGGCATAAATAACTGTACACAAGGCAGTTAAATACACAGTGGTGCGATAGC
ACCTCCTGGTGGTCACTTGAAGTGGTGCACTCAGCAGCAAGTTCCATCTTTCACCCACTT
GCCAGGCCTGGCATTTAAGATTTTGTCTCCAAGGGACCAGGGAGGCCCCAGTTTGAGAGA
GGAGGCTCCGATCAATCCAGGGTGCTACTTGTTAGGAGGTCAAGTAAAGGGCTTGATGGG
GAGCAACGTTGAGGAAATAAGACTTGAGGCTGGGGGATATCACTTTCCTCCATCTCCCCA
GGCCCTGTGACCTCAGATAATGCAGAGCTGGCTCCATCCTTAAGTTCTGTGCCCGCAGCT
TTAACCCCTCCTGTANGCCCTCAGAGCGCTGACATCTCCAGGCTGCTGGGCTGCACCGNC
ACCCTGTCCTTCTTCGCCTCGTAAGGAAATACAGCACCAAAGATATCTTCGTGATAGCGC
TCCTGGCTCTTGGACTGAAAGAAATAGTCCTCGACCTGCTCCTCATTCATTTCAGCTTGG
CAGCCACCAGCTGCTTCAGGGGTGTGGGCCACGTTCCGGGCCATGCCCACCATCCCGGAA
AACATAGAAGTTGCCTGGCTTCTTGTGGAACACAAGGACCACTTGCTTGGCCACTGCTGC
CGAAGGAAGTCCTGAAACTAAACCTGGN
Restriction Sites NotI-NotI     
ACCN NM_000625
Insert Size 4100 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation There is 2 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 2 aa.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000625.3, NP_000616.3
RefSeq Size 4221 bp
RefSeq ORF 3462 bp
Locus ID 4843
Cytogenetics 17q11.2
Protein Families Druggable Genome
Protein Pathways Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer
Gene Summary 'Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]'

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