CACNA1B (NM_000718) Human Untagged Clone

CAT#: SC300124

CACNA1B (untagged)-Human calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CACNA1B
• Synonyms: BIII; CACNL1A5; CACNN; Cav2.2; DYT23; NEDNEH
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000718 edited
  
  
  AGCCATGGTCCGCTTCGGGGACGAGCTGGGCGGCCGCTATGGAGGCCCCGGCGGCGGAGAGCGGGCCCGG
  GGCGGCGGGGCCGGCGGGGCGGGGGGCCCGGGTCCCGGGGGGCTGCAGCCCGGCCAGCGGGTCCTCTACA
  AGCAATCGATCGCGCAGCGCGCGCGGACCATGGCGCTGTACAACCCCATCCCGGTCAAGCAGAACTGCTT
  CACCGTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATACGCGAAGCGCATCACC
  GAGTGGCCTCCATTCGAGTATATGATCCTGGCCACCATCATCGCCAACTGCATCGTGCTGGCCCTGGAGC
  AGCACCTCCCTGATGGGGACAAAACGCCCATGTCCGAGCGGCTGGACGACACGGAGCCCTATTTCATCGG
  GATCTTTTGCTTCGAGGCAGGGATCAAAATCATCGCTCTGGGCTTTGTCTTCCACAAGGGCTCTTACCTG
  CGGAACGGCTGGAACGTCATGGACTTCGTGGTCGTCCTCACAGGGATCCTTGCCACGGCTGGAACTGACT
  TCGACCTGCGAACACTGAGGGCTGTGCGTGTGCTGAGGCCCCTGAAGCTGGTGTCTGGGATTCCAAGTTT
  GCAGGTGGTGCTCAAGTCCATCATGAAGGCCATGGTTCCACTCCTGCAGATTGGGCTGCTTCTCTTCTTT
  GCCATCCTCATGTTTGCCATCATTGGCCTGGAGTTCTACATGGGCAAGTTCCACAAGGCCTGTTTCCCCA
  ACAGCACAGATGCGGAGCCCGTGGGTGACTTCCCCTGTGGCAAGGAGGCCCCAGCCCGGCTGTGCGAGGG
  CGACACTGAGTGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAACTTTGACAATATCCTGTTT
  GCCATCTTGACGGTGTTCCAGTGCATCACCATGGAGGGCTGGACTGACATCCTCTATAATACAAACGATG
  CGGCCGGCAACACCTGGAACTGGCTCTACTTCATCCCTCTCATCATCATCGGCTCCTTCTTCATGCTCAA
  CCTGGTGCTGGGCGTGCTCTCGGGGGAGTTTGCCAAGGAGCGAGAGAGGGTGGAGAACCGCCGCGCCTTC
  CTGAAGCTGCGCCGGCAGCAGCAGATCGAGCGAGAGCTCAACGGGTACCTGGAGTGGATCTTCAAGGCGG
  AGGAAGTCATGCTGGCCGAGGAGGACAGGAATGCAGAGGAGAAGTCCCCTTTGGACGTGCTGAAGAGAGC
  GGCCACCAAGAAGAGCAGAAATGACCTGATCCACGCAGAGGAGGGAGAGGACCGGTTTGCAGATCTCTGT
  GCTGTTGGATCCCCCTTCGCCCGCGCCAGCCTCAAGAGCGGGAAGACAGAGAGCTCGTCATACTTCCGGA
  GGAAGGAGAAGATGTTCCGGTTTTTTATCCGGCGCATGGTGAAGGCTCAGAGCTTCTACTGGGTGGTGCT
  GTGCGTGGTGGCCCTGAACACACTGTGTGTGGCCATGGTGCATTACAACCAGCCGCGGCGGCTTACCACG
  ACCCTGTATTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTATGGCC
  TGGGGCCCAGAAGCTACTTCCGGTCCTCCTTCAACTGCTTCGACTTTGGGGTCATCGTGGGGAGCGTCTT
  TGAAGTGGTCTGGGCGGCCATCAAGCCGGGAAGCTCCTTTGGGATCAGTGTGCTGCGGGCCCTCCGCCTG
  CTGAGGATCTTCAAAGTCACGAAGTACTGGAGCTCCCTGCGGAACCTGGTGGTGTCCCTGCTGAACTCCA
  TGAAGTCCATCATCAGCCTGCTCTTCTTGCTCTTCCTGTTCATTGTGGTCTTCGCCCTGCTGGGGATGCA
  GCTGTTTGGGGGACAGTTCAACTTCCAGGATGAGACTCCCACAACCAACTTCGACACCTTCCCTGCCGCC
  ATCCTCACTGTCTTCCAGATCCTGACGGGAGAGGACTGGAATGCAGTGATGTATCACGGGATCGAATCGC
  AAGGCGGCGTCAGCAAAGGCATGTTCTCGTCCTTTTACTTCATTGTCCTGACACTGTTCGGAAACTACAC
  TCTGCTGAATGTCTTTCTGGCCATCGCTGTGGACAACCTGGCCAACGCCCAAGAGCTGACCAAGGATGAA
  GAGGAGATGGAAGAAGCAGCCAATCAGAAGCTTGCTCTGCAAAAGGCCAAAGAAGTGGCTGAAGTCAGCC
  CCATGTCTGCCGCGAACATCTCCATCGCCGCCAGGCAGCAGAACTCGGCCAAGGCGCGCTCGGTGTGGGA
  GCAGCGGGCCAGCCAGCTCCGGCTGCAGAACCTGCGGGCCAGCTGCGAGGCGCTGTACAGCGAGATGGAC
  CCCGAGGAGCGGCTGCGCTTCGCCACTACGCGCCACCTGCGGCCCGACATGAAGACGCATCTGGACCGGC
  CGCTGGTGGTGGAGCTGGGCCGCGACGGCGCGCGGGGCCCCGTGGGAGGCAAAGCCCGACCTGAGGCTGC
  GGAGGCCCCCGAGGGCGTCGACCCTCCGCGCAGGCACCACCGGCACCGCGACAAGGACAAGACCCCCGCG
  GCGGGGGACCAGGACCGAGCAGAGGCCCCGAAGGCGGAGAGCGGGGAGCCCGGTGCCCGGGAGGAGCGGC
  CGCGGCCGCACCGCAGCCACAGCAAGGAGGCCGCGGGGCCCCCGGAGGCGCGGAGCGAGCGCGGCCGAGG
  CCCAGGCCCCGAGGGCGGCCGGCGGCACCACCGGCGCGGCTCCCCGGAGGAGGCGGCCGAGCGGGAGCCC
  CGACGCCACCGCGCGCACCGGCACCAGGATCCGAGCAAGGAGTGCGCCGGCGCCAAGGGCGAGCGGCGCG
  CGCGGCACCGCGGCGGCCCCCGAGCGGGGCCCCGGGAGGCGGAGAGCGGGGAGGAGCCGGCGCGGCGGCA
  CCGGGCCCGGCACAAGGCGCAGCCTGCTCACGAGGCTGTGGAGAAGGAGACCACGGAGAAGGAGGCCACG
  GAGAAGGAGGCTGAGATAGTGGAAGCCGACAAGGAAAAGGAGCTCCGGAACCACCAGCCCCGGGAGCCAC
  ACTGTGACCTGGAGACCAGTGGGACTGTGACTGTGGGTCCCATGCACACACTGCCCAGCACCTGTCTCCA
  GAAGGTGGAGGAACAGCCAGAGGATGCAGACAATCAGCGGAACGTCACTCGCATGGGCAGTCAGCCCCCA
  GACCCGAACACTATTGTACATATCCCAGTGATGCTGACGGGCCCTCTTGGGGAAGCCACGGTCGTTCCCA
  GTGGTAACGTGGACCTGGAAAGCCAAGCAGAGGGGAAGAAGGAGGTGGAAGCGGATGACGTGATGAGGAG
  CGGCCCCCGGCCTATCGTCCCATACAGCTCCATGTTCTGTTTAAGCCCCACCAACCTGCTCCGCCGCTTC
  TGCCACTACATCGTGACCATGAGGTACTTCGAGGTGGTCATTCTCGTGGTCATCGCCTTGAGCAGCATCG
  CCCTGGCTGCTGAGGACCCAGTGCGCACAGACTCGCCCAGGAACAACGCTCTGAAATACCTGGATTACAT
  TTTCACTGGTGTCTTTACCTTTGAGATGGTGATAAAGATGATCGACTTGGGACTGCTGCTTCACCCTGGA
  GCCTATTTCCGGGACTTGTGGAACATTCTGGACTTCATTGTGGTCAGTGGCGCCCTGGTGGCGTTTGCTT
  TCTCAGGATCCAAAGGGAAAGACATCAATACCATCAAGTCTCTGAGAGTCCTTCGTGTCCTGCGGCCCCT
  CAAGACCATCAAACGGCTGCCCAAGCTCAAGGCTGTGTTTGACTGTGTGGTGAACTCCCTGAAGAATGTC
  CTCAACATCTTGATTGTCTACATGCTCTTCATGTTCATATTTGCCGTCATTGCGGTGCAGCTCTTCAAAG
  GGAAGTTTTTCTACTGCACAGATGAATCCAAGGAGCTGGAGAGGGACTGCAGGGGTCAGTATTTGGATTA
  TGAGAAGGAGGAAGTGGAAGCTCAGCCCAGGCAGTGGAAGAAATACGACTTTCACTACGACAATGTGCTC
  TGGGCTCTGCTGACGCTGTTCACAGTGTCCACGGGAGAAGGCTGGCCCATGGTGCTGAAACACTCCGTGG
  ATGCCACCTATGAGGAGCAGGGTCCAAGCCCTGGGTACCGCATGGAGCTGTCCATCTTCTACGTGGTCTA
  CTTTGTGGTCTTTCCCTTCTTCTTCGTCAACATCTTTGTGGCTTTGATCATCATCACCTTCCAGGAGCAG
  GGGGACAAGGTGATGTCTGAATGCAGCCTAGAGAAGAACGAGAGGGCTTGCATTGACTTCGCCATCAGCG
  CCAAACCCCTGACACGGTACATGCCCCAAAACCGGCAGTCGTTCCAGTATAAGACGTGGACATTTGTGGT
  CTCCCCGCCCTTTGAATACTTCATCATGGCCATGATAGCCCTCAACACTGTGGTGCTGATGATGAAGTTC
  TATGATGCACCCTATGAGTACGAGCTGATGCTGAAATGCCTGAACATCGTGTTCACATCCATGTTCTCCA
  TGGAATGCGTGCTGAAGATCATCGCCTTTGGGGTGCTGAACTATTTCAGAGATGCCTGGAATGTCTTTGA
  CTTTGTCACTGTGTTGGGAAGTATTACTGATATTTTAGTAACAGAGATTGCGAACAATTTCATCAACCTC
  AGCTTCCTCCGCCTCTTTCGAGCTGCGCGGCTGATCAAGCTGCTCCGCCAGGGCTACACCATCCGCATCC
  TGCTGTGGACCTTTGTCCAGTCCTTCAAGGCCCTGCCCTACGTGTGTCTGCTCATTGCCATGCTGTTCTT
  CATCTACGCCATCATCGGCATGCAGGTGTTTGGGAATATTGCCCTGGATGATGACACCAGCATCAACCGC
  CACAACAACTTCCGGACGTTTTTGCAAGCCCTGATGCTGCTGTTCAGGAGCGCCACGGGGGAGGCCTGGC
  ACGAGATCATGCTGTCCTGCCTGAGCAACCAGGCCTGTGATGAGCAGGCCAATGCCACCGAGTGTGGAAG
  TGACTTTGCCTACTTCTACTTCGTCTCCTTCATCTTCCTGTGCTCCTTTCTGATGTTGAACCTCTTTGTG
  GCTGTGATCATGGACAATTTTGAGTACCTCACGCGGGACTCTTCCATCCTAGGTCCTCACCACTTGGATG
  AGTTCATCCGGGTCTGGGCTGAATACGACCCGGCTGCGTGTGGGCGCATCAGTTACAATGACATGTTTGA
  GATGCTGAAACACATGTCCCCGCCTCTGGGGCTGGGGAAGAAATGCCCTGCTCGAGTTGCTTACAAGCGC
  CTGGTTCGCATGAACATGCCCATCTCCAACGAGGACATGACTGTTCACTTCACGTCCACGCTGATGGCCC
  TCATCCGGACGGCACTGGAGATCAAGCTGGCCCCAGCTGGGACAAAGCAGCATCAGTGTGACGCGGAGTT
  GAGGAAGGAGATTTCCGTTGTGTGGGCCAATCTGCCCCAGAAGACTTTGGACTTGCTGGTACCACCCCAT
  AAGCCTGATGAGATGACAGTGGGGAAGGTTTATGCAGCTCTGATGATATTCGACTTCTACAAGCAGAACA
  AAACCACCAGAGACCAGATGCAGCAGGCTCCTGGAGGCCTCTCCCAGATGGGTCCTGTGTCCCTGTTCCA
  CCCTCTGAAGGCCACCCTGGAGCAGACACAGCCGGCTGTGCTCCGAGGAGCCCGGGTTTTCCTTCGACAG
  AAGAGTTCCACCTCCCTCAGCAATGGCGGGGCCATACAAAACCAAGAGAGTGGCATCAAAGAGTCTGTCT
  CCTGGGGCACTCAAAGGACCCAGGATGCACCCCATGAGGCCAGGCCACCCCTGGAGCGTGGCCACTCCAC
  AGAGATCCCTGTGGGGCGGTCAGGAGCACTGGCTGTGGACGTTCAGATGCAGAGCATAACCCGGAGGGGC
  CCTGATGGGGAGCCCCAGCCTGGGCTGGAGAGCCAGGGTCGAGCGGCCTCCATGCCCCGCCTTGCGGCCG
  AGACTCAGCCCGTCACAGATGCCAGCCCCATGAAGCGCTCCATCTCCACGCTGGCCCAGCGGCCCCGTGG
  GACTCATCTTTGCAGCACCACCCCGGACCGCCCACCCCCTAGCCAGGCGTCGTCGCACCACCACCACCAC
  CGCTGCCACCGCCGCAGGGACAGGAAGCAGAGGTCCCTGGAGAAGGGGCCCAGCCTGTCTGCCGATATGG
  ATGGCGCACCAAGCAGTGCTGTGGGGCCGGGGCTGCCCCCGGGAGAGGGGCCTACAGGCTGCCGGCGGGA
  ACGAGAGCGCCGGCAGGAGCGGGGCCGGTCCCAGGAGCGGAGGCAGCCCTCATCCTCCTCCTCGGAGAAG
  CAGCGCTTCTACTCCTGCGACCGCTTTGGGGGCCGTGAGCCCCCGAAGCCCAAGCCCTCCCTCAGCAGCC
  ACCCAACGTCGCCAACAGCTGGCCAGGAGCCGGGACCCCACCCACAGGGCAGTGGTTCCGTGAATGGGAG
  CCCCTTGCTGTCAACATCTGGTGCTAGCACCCCCGGCCGCGGTGGGCGGAGGCAGCTCCCCCAGACGCCC
  CTGACTCCCCGCCCCAGCATCACCTACAAGACGGCCAACTCCTCACCCATCCACTTCGCCGGGGCTCAGA
  CCAGCCTCCCTGCCTTCTCCCCAGGCCGGCTCAGCCGTGGGCTTTCCGAACACAACGCCCTGCTGCAGAG
  AGACCCCCTCAGCCAGCCCCTGGCCCCTGGCTCTCGAATTGGCTCTGACCCTTACCTGGGGCAGCGTCTG
  GACAGTGAGGCCTCTGTCCACGCCCTGCCTGAGGACACGCTCACTTTCGAGGAGGCTGTGGCCACCAACT
  CGGGCCGCTCCTCCAGGACTTCCTACGTGTCCTCCCTGACCTCCCAGTCTCACCCTCTCCGCCGCGTGCC
  CAACGGTTACCACTGCACCCTGGGACTCAGCTCGGGTGGCCGAGCACGGCACAGCTACCACCACCCTGAC
  CAAGACCACTGGTGCTAGCTGCACCGTGACCGCTCAGACGCCTGCATGCAGCAGGCGTGTGTTCCAGTGG
  ATGAGTTTTATCATCCACACGGGGCAGTCGGCCCTCGGGGGAGGCCTTGCCCACCTTGGTGAGGCTCCTG
  TGGCCCCTCCCTCCCCCTCCTCCCCTTTTTTACTCTAGACGACGAATAAAGCCCTGTTAGAGGAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000718 unedited
  GGCAGACATTTTTTTACGACTCACTATTAGGGACGGCCGCGAATTCGAGCCATGGTCCGC
  TTCGGGGACGAGCTGGGCGGCCGCTATGGAGGCCCCGGCGGCGGAGAGCGGGCCCGGGGC
  GGCGGGGCCGGCGGGGCGGGGGGCCCGGGTCCCGGGGGGCTGCAGCCCGGCCAGCGGGTC
  CTCTACAAGCAATCGATCGCGCAGCGCGCGCGGACCATGGCGCTGTACAACCCCATCCCG
  GTCAAGCAGAACTGCTTCACCGTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTC
  GTCCGCAAATACGCGAAGCGCATCACCGAGTGGCCTCCATTCGAGTATATGATCCTGGCC
  ACCATCATCGCCAACTGCATCGTGCTGGCCCTGGAGCAGCACCTCCCTGATGGGGACAAA
  ACGCCCATGTCCGAGCGGCTGGACGACACGGAGCCCTATTTCATCGGGATCTTTTGCTTC
  GAGGCAGGGATCAAAATCATCGCTCTGGGCTTTGTCTTCCACAAGGGCTCTTACCTGCGG
  AACGGCTGGAACGTCATGGACTTCGTGGTCGTCCTCACAGGGATCCTTGCCACGGCTGGA
  ACTGACTTCGACCTGCGAACACTGAAGGCTGTGCGTGTGCTGAGGCCCCTGAACTTGTGG
  CTGGGTTCCAAGTTGCAGTGGTGCTCAATCCATCATGAGGCCATGTTCCCCTCCCTGCAA
  TGGGCTGTTTTTTTCTTTGCCTCCTTATGTTTGCCCATCTTGGCCTGGATTTTACAGGGG
  CAGTTCCACAAGCCTGGTTCCCCACAGCCAAGATGCCGAACCCGTGGTGACATTCCCCGG
  GGCAAGGAAGCCCCCAACCCGGCTGGGCAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000718 unedited
  GNGNGAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGTTCTGTTCAGGNAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCTAACAGGGCTTTTTTCTTCGTCTAAA
  GTAAAAAAGGGAAGGAGGGGGAGGGAGGGCCCACAGGACCCTCACCAAGGTGGGCAAGGC
  CTCCCCCGAGGGCCGACTGCCCCGTGTGGATGATAAAACTCATCCACTGGAACACACCCC
  TGCTGCTTGCAGGCTTTCTGAGCGGTCACGGTGCATCTAGCACCAATGTTCTTGGTCAGG
  GTGGTGGTACCTGTCCCGTGCTCGCCCCCCGAGCTGGGTCCCAGGGTGCATTGTAACCCT
  TGGGCCCCCGCCGGAAAGGTTAAACTGGAAGGTCAGGGAGCCACCTATGAATTCCTGGAG
  ACCCGCCCAGTTTTGGCCCCACCTCTTCAAAGTTGCCTTTTCCCAGGCAGGCTTGCCCAG
  GCCCTACTTTCCAACCCCTCCCCCGTTAAGGTTAAACCCTTTTCAAACCCCGGCCCCAGC
  CCTTGTTTAGGGGTTCTTTTTTCCCAAAGGGCTTTTTTTTTGAAAACCCCCCGTTTACCC
  CCCCCTTGGAAAAAAACCAGGAGGCTTTTTTTTTCCCCCCCGCAAAATTTTTTGGTTAAA
  GATTTTCCCCCTTTTTAAGTATTCCTGGGGCCGGGATTTACGGCCCCTTTTGGGGACTCT
  CCCCCCCCCCCCCGGCCCGGGGGTT
  
• Restriction Sites: Please inquire
• ACCN: NM_000718
• Insert Size: 7300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000718.1, NP_000709.1
• RefSeq Size: 7364 bp
• RefSeq ORF: 7020 bp
• Locus ID: 774
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Calcium signaling pathway, MAPK signaling pathway, Taste transduction, Type II diabetes mellitus
• Gene Summary: 'The protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel, which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2, beta, and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.