CHRNB3 (NM_000749) Human Untagged Clone

CAT#: SC300130

CHRNB3 (untagged)-Human cholinergic receptor, nicotinic, beta 3 (CHRNB3)


  "NM_000749" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CHRNB3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000749 edited
TGTTGCTGTCCTCTTGGGTTCCACTTCGGATTTTGAACCCCTGTATTTTCTTTTCAAAAC
CCCCTTTTCCAGTGGAAATGCTCTGTTGTTAAAAAGGAAGAAACTGTCTTTCTGAAACTG
ACATCACGATGCTCCCAGATTTTATGCTGGTTCTCATCGTCCTTGGCATCCCTTCCTCAG
CCACCACAGGTTTCAACTCAATCGCCGAAAATGAAGATGCCCTCCTCAGACATTTGTTCC
AAGGTTATCAGAAATGGGTCCGCCCTGTATTACATTCTAATGACACCATAAAAGTATATT
TTGGATTGAAAATATCCCAGCTTGTAGATGTGGATGAAAAGAATCAGCTGATGACAACCA
ATGTGTGGCTCAAACAGGAATGGACAGACCACAAGTTACGCTGGAATCCTGATGATTATG
GTGGGATCCATTCCATTAAAGTTCCATCAGAATCTCTGTGGCTTCCTGACATAGTTCTCT
TTGAAAATGCTGACGGCCGCTTCGAAGGCTCCCTGATGACCAAGGTCATCGTGAAATCAA
ACGGAACTGTTGTCTGGACCCCTCCCGCCAGCTACAAAAGCTCCTGCACCATGGACGTCA
CGTTTTTCCCGTTCGACCGACAGAACTGCTCCATGAAGTTTGGATCCTGGACTTATGATG
GCACCATGGTTGACCTCATTTTGATCAATGAAAATGTCGACAGAAAAGACTTCTTCGATA
ACGGAGAATGGGAAATACTGAATGCAAAGGGGATGAAGGGGAACAGAAGGGACGGCGTGT
ACTCCTATCCCTTTATCACGTATTCCTTCGTCCTGAGACGCCTGCCTTTATTCTATACCC
TCTTTCTCATCATCCCCTGCCTGGGGCTGTCTTTCCTAACAGTTCTTGTGTTCTATTTAC
CTTCGGATGAAGGAGAAAAACTTTCATTATCCACATCGGTCTTGGTTTCTCTGACAGTTT
TCCTTTTAGTGATTGAAGAAATCATCCCATCGTCTTCCAAAGTCATTCCTCTCATTGGAG
AGTACCTGCTGTTCATCATGATTTTTGTGACCCTGTCCATCATTGTTACCGTGTTTGTCA
TTAACGTTCACCACAGATCTTCTTCCACGTACCACCCCATGGCCCCCTGGGTTAAGAGGC
TCTTTCTGCAGAAACTTCCAAAATTACTTTGCATGAAAGATCATGTGGATCGCTACTCAT
CCCCAGAGAAAGAGGAGAGTCAACCAGTAGTGAAAGGCAAAGTCCTCGAAAAAAAGAAAC
AGAAACAGCTTAGTGATGGAGAAAAAGTTCTAGTTGCTTTTTTGGAAAAAGCTGCTGATT
CCATTAGATACATTTCCAGACATGTGAAGAAAGAACATTTTATCAGCCAGGTAGTACAAG
ACTGGAAATTTGTAGCTCAAGTTCTTGACCGAATCTTCCTGTGGCTCTTTCTGATAGTGT
CAGTAACAGGCTCGGTTCTGATTTTTACCCCTGCTTTGAAGATGTGGCTACATAGTTACC
ATTAGGAATTTAAAAGACATAAGACTAAATTACACCTTAGACCTGACATCTGGCTATCAC
ACAGACAGAATCCAAATGC
Restriction Sites Please inquire     
ACCN NM_000749
Insert Size 1600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000749.2, NP_000740.1
RefSeq Size 1956 bp
RefSeq ORF 1377 bp
Locus ID 1142
Cytogenetics 8p11.21
Protein Families Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
Gene Summary 'The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.[supplied by OMIM, Apr 2010]'
Transcript Variant: This variant (1) encodes the longer isoform (1).

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