Pancreatic Lipase (PNLIP) (NM_000936) Human Untagged Clone

CAT#: SC300155

PNLIP (untagged)-Human pancreatic lipase (PNLIP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PNLIP
• Synonyms: PL; PNLIPD; PTL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000936, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGCCACTTTGGACTCTTTCACTGCTGCTGGGAGCAGTAGCAGGAAAAGAAGTTTGCTACGAAAGAC
  TCGGCTGCTTCAGTGATGACTCCCCATGGTCAGGAATTACGGAAAGACCCCTCCATATATTGCCTTGGTC
  TCCAAAAGATGTCAACACCCGCTTCCTCCTATATACTAATGAGAACCCAAACAACTTTCAAGAAGTTGCC
  GCAGATTCATCAAGCATCAGTGGCTCCAATTTCAAAACAAATAGAAAAACTCGCTTTATTATTCATGGAT
  TCATAGACAAGGGAGAAGAAAACTGGCTGGCCAATGTGTGCAAGAATCTGTTCAAGGTGGAAAGTGTGAA
  CTGTATCTGTGTGGACTGGAAAGGTGGCTCCCGAACTGGATACACACAAGCCTCGCAGAACATCAGGATC
  GTGGGAGCAGAAGTGGCATATTTTGTTGAATTTCTTCAGTCGGCGTTCGGTTACTCACCTTCCAACGTGC
  ATGTCATTGGCCACAGCCTGGGTGCCCACGCTGCTGGGGAGGCTGGAAGGAGAACCAATGGGACCATTGG
  ACGCATCACAGGGTTGGACCCAGCAGAACCTTGCTTTCAGGGCACACCTGAATTAGTCCGATTGGACCCC
  AGCGATGCCAAATTTGTGGATGTAATTCACACGGATGGTGCCCCCATAGTCCCCAATTTGGGGTTTGGAA
  TGAGCCAAGTCGTGGGCCACCTAGATTTCTTTCCAAATGGAGGAGTGGAAATGCCTGGATGTAAAAAGAA
  CATTCTCTCTCAGATTGTGGACATAGACGGAATCTGGGAAGGGACTCGAGACTTTGCGGCCTGTAATCAC
  TTAAGAAGCTACAAATATTACACTGATAGCATCGTCAACCCTGATGGCTTTGCTGGATTCCCCTGTGCCT
  CTTACAACGTCTTCACTGCAAACAAGTGTTTCCCTTGTCCAAGTGGAGGCTGCCCACAGATGGGTCACTA
  TGCTGATAGATATCCTGGGAAAACAAATGATGTGGGCCAGAAATTTTATCTAGACACTGGTGATGCCAGT
  AATTTTGCACGTTGGAGGTATAAGGTATCTGTCACACTGTCTGGAAAAAAGGTTACAGGACACATACTAG
  TTTCTTTGTTCGGAAATAAAGGAAACTCTAAGCAGTATGAAATTTTCAAGGGCACTCTCAAACCAGATAG
  TACTCATTCCAATGAATTTGACTCAGATGTGGATGTTGGGGACTTGCAGATGGTTAAATTTATTTGGTAT
  AACAATGTGATCAACCCAACTTTACCTAGAGTGGGAGCATCCAAGATTATAGTGGAGACAAATGTTGGAA
  AACAGTTCAACTTCTGTAGTCCAGAAACCGTCAGGGAGGAAGTTCTGCTCACCCTCACACCGTGTTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000936
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000936.3, NP_000927.1
• RefSeq Size: 1510 bp
• RefSeq ORF: 1398 bp
• Locus ID: 5406
• Cytogenetics: 10q25.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the lipase family of proteins. The encoded enzyme is secreted by the pancreas and hydrolyzes triglycerides in the small intestine, and is essential for the efficient digestion of dietary fats. Inhibition of the encoded enzyme may prevent high-fat diet-induced obesity in mice and result in weight loss in human patients with obesity. Mutations in this gene cause congenital pancreatic lipase deficiency, a rare disorder characterized by steatorrhea. [provided by RefSeq, Jul 2016]'