GGA1 (NM_001001560) Human Untagged Clone

CAT#: SC300217

GGA1 (untagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GGA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001001560, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCCCGCGATGGAGCCGGAGACTCTGGAGGCGCGAATCAATAGAGCCACGAACCCCCTGAACAAGG
  AGCTCGACTGGGCCAGCATCAACGGCTTCTGCGAGCAGCTCAACGAGGACTTTGAGGGGCCTCCACTCGC
  CACCCGGCTGCTGGCCCACAAGATCCAGTCCCCACAGGAGTGGGAGGCGATCCAGGCCTTGACGGTGCTG
  GAAACATGCATGAAGAGCTGCGGCAAGCGGTTCCACGACGAAGTGGGCAAGTTCCGCTTTCTCAACGAGC
  TCATCAAGGTCGTGTCTCCCAAGTATCTGGGCTCTCGGACATCGGAGAAGGTGAAGAACAAGATCTTGGA
  GCTCCTCTACAGCTGGACAGTGGGCCTGCCCGAGGAGGTGAAAATCGCAGAGGCCTACCAGATGCTAAAG
  AAGCAGGGGATTGTAAAGTCCGACCCCAAGCTTCCAGATGACACTACCTTTCCCCTTCCTCCTCCACGGC
  CGAAGAATGTGATCTTTGAAGATGAGGAGAAATCCAAGATGCTGGCCCGCCTGCTGAAGAGCTCCCATCC
  CGAAGACCTCCGCGCAGCCAATAAGCTCATCAAAGAGATGGTGCAGGAGGACCAGAAGCGGATGGAGAAG
  ATCTCGAAGAGGGTGAATGCCATCGAGGAGGTGAACAACAATGTGAAACTGCTCACGGAGATGGTGATGA
  GCCACAGCCAGGGCGGCGCAGCAGCTGGCAGCAGCGAGGACCTCATGAAGGAACTGTACCAGCGCTGTGA
  GCGGATGCGGCCCACGCTCTTCCGACTGGCGAGTGACACAGAGGACAATGATGAGGCCTTAGGCCTCAGT
  GACCCCACACCCCCTTCAGGCCCAAGCCTGGATGGTACCGGATGGAACAGCTTCCAGTCGTCGGATGCCA
  CTGAGCCCCCAGCCCCTGCTCTGGCCCAGGCCCCCAGTATGGAAAGCCGACCCCCAGCGCAGACATCCCT
  GCCAGCAAGCAGCGGTCTGGACGACCTAGACCTCCTGGGGAAGACCCTCCTGCAGCAGTCGCTGCCCCCG
  GAATCCCAGCAAGTGCGGTGGGAGAAGCAGCAGCCAACCCCCCGGCTCACACTCCGGGACCTGCAGAATA
  AGAGCAGCAGCTGCAGCTCCCCCAGCTCCAGCGCCACCAGCCTTCTCCACACCGTGTCCCCAGAGCCCCC
  CAGGCCTCCGCAGCAGCCCGTACCAACCGAGCTCTCACTGGCCAGCATCACTGTGCCCCTGGAGTCCATC
  AAACCCAGCAACATCCTGCCCGTGACTGTGTATGACCAGCACGGCTTCCGCATCCTCTTCCATTTTGCCC
  GGGACCCACTGCCAGGGCGCTCCGACGTGCTGGTGGTGGTGGTTTCCATGCTGAGCACCGCCCCCCAGCC
  CATCCGCAACATCGTGTTCCAGTCAGCTGTCCCCAAGGTTATGAAGGTGAAGCTGCAGCCACCCTCGGGC
  ACGGAGCTGCCAGCTTTTAACCCCATCGTCCACCCCTCAGCAATCACCCAGGTCCTGCTGCTTGCCAACC
  CCCAGAAGGAGAAGGTTCGCCTCCGCTACAAGCTCACCTTCACCATGGGTGACCAGACCTACAACGAGAT
  GGGGGATGTGGACCAGTTCCCCCCACCTGAAACCTGGGGTAGCCTCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001001560
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001560.2, NP_001001560.1
• RefSeq Size: 2924 bp
• RefSeq ORF: 1659 bp
• Locus ID: 26088
• Cytogenetics: 22q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• Gene Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks 2 in-frame exons in the coding region, as compared to variant 1. The encoded isoform 2 thus lacks an internal segment, as compared to isoform 1.