USP16 (NM_001001992) Human Untagged Clone

CAT#: SC300379

USP16 (untagged)-Human ubiquitin specific peptidase 16 (USP16), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP16
• Synonyms: UBP-M; UBPM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001001992, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAAAGAAACGGACAAAGGGAAAAACTGTTCCAATCGATGATTCCTCTGAAACTTTAGAACCTGTGT
  GCAGACACATTAGAAAAGGATTGGAACAAGGTAATTTGAAAAAGGCTTTAGTGAATGTGGAATGGAATAT
  CTGCCAAGACTGTAAGACTGACAATAAAGTGAAAGATAAAGCTGAAGAAGAAACAGAAGAAAAGCCTTCA
  GTTTGGCTGTGTCTTAAATGTGGCCATCAGGGCTGTGGCAGAAATTCTCAGGAGCAGCATGCCTTGAAGC
  ACTATCTGACGCCAAGATCTGAACCTCACTGTCTGGTTCTTAGTTTGGACAACTGGAGTGTATGGTGTTA
  CGTATGTGATAATGAGGTCCAGTATTGTAGTTCAAACCAGTTGGGTCAAGTGGTTGATTATGTCAGAAAA
  CAAGCCAGCATTACAACTCCAAAGCCAGAGAAAGATAATGGAAATATTGAACTTGAAAATAAAAAATTAG
  AAAAAGAGAGTAAGAATGAACAAGAGAGAGAAAAGAAGGAAAACATGGCTAAAGAGAATCCTCCCATGAA
  TTCTCCTTGCCAAATAACCGTGAAAGGACTCAGTAATTTGGGAAACACATGTTTCTTCAATGCAGTTATG
  CAGAACTTGTCACAAACACCAGTGCTTAGAGAACTACTAAAAGAAGTGAAAATGTCTGGAACAATTGTAA
  AAATTGAACCACCTGATTTGGCATTAACAGAACCATTAGAAATAAACCTTGAGCCTCCAGGCCCTCTTAC
  TTTAGCCATGAGCCAGTTTCTTAATGAGATGCAAGAGACCAAAAAGGGGGTTGTGACACCGAAAGAACTC
  TTTTCTCAGGTCTGTAAAAAAGCAGTGCGGTTTAAAGGCTATCAGCAGCAAGACAGCCAGGAGCTGCTTC
  GCTACTTATTGGATGGGATGAGAGCAGAAGAACACCAAAGAGTGAGTAAAGGAATACTTAAAGCATTTGG
  TAATTCTACTGAAAAGTTGGATGAAGAACTAAAAAATAAAGTTAAAGATTATGAGAAGAAAAAATCAATG
  CCAAGTTTTGTTGACCGCATCTTTGGTGGTGAACTAACTAGTATGATCATGTGTGATCAATGCAGAACTG
  TCTCCTTGGTTCATGAATCTTTCCTTGATTTGTCCCTCCCAGTTTTAGATGATCAGAGTGGTAAGAAAAG
  TGTAAATGATAAAAATCTGAAAAAGACAGTGGAGGATGAAGATCAAGATAGTGAGGAAGAAAAAGATAAC
  GACAGTTACATAAAAGAGAGAAGTGATATTCCTTCTGGAACAAGTAAGCACTTACAGAAAAAAGCAAAGA
  AACAAGCCAAAAAGCAAGCCAAGAACCAACGAAGACAACAAAAAATTCAAGGAAAAGTTCTTCATTTAAA
  TGATATTTGTACTATTGACCATCCTGAAGACAGTGAATATGAAGCTGAAATGTCACTTCAAGGAGAAGTA
  AATATTAAATCCAACCATATTTCACAAGAGGGTGTTATGCATAAAGAATATTGTGTCAACCAGAAAGATT
  TGAATGGCCAAGCAAAAATGATCGAAAGTGTAACTGACAATCAAAAATCCACAGAGGAAGTAGATATGAA
  AAATATCAACATGGATAATGATCTGGAGGTTTTAACATCTTCTCCCACTAGGAATTTAAATGGTGCCTAC
  CTAACGGAAGGGAGCAATGGAGAAGTGGACATTTCCAATGGTTTCAAAAACCTAAATTTGAATGCTGCTC
  TTCATCCTGATGAAATAAATATAGAGATTCTGAATGATAGTCATACTCCTGGAACAAAGGTGTATGAGGT
  TGTAAATGAAGATCCAGAAACTGCTTTCTGTACTCTTGCAAACAGGGAAGTTTTCAATACTGATGAGTGT
  TCAATCCAACATTGTTTATATCAGTTCACCCGTAATGAGAAACTTCGAGATGCGAATAAACTGCTTTGTG
  AAGTATGCACACGGAGACAGTGTAATGGACCAAAGGCAAATATAAAAGGTGAAAGGAAGCATGTTTACAC
  CAATGCCAAAAAGCAGATGCTAATTTCTCTTGCTCCTCCTGTTCTTACTCTTCATTTAAAGAGATTTCAG
  CAGGCTGGTTTTAACCTACGCAAAGTTAACAAACACATAAAGTTTCCGGAAATCTTAGATTTGGCTCCTT
  TTTGCACCCTTAAATGTAAGAATGTTGCAGAAGAAAATACAAGGGTACTCTATTCCTTATATGGAGTTGT
  TGAACACAGTGGTACTATGAGGTCGGGGCATTACACTGCCTATGCCAAGGCAAGAACCGCAAATAGTCAT
  CTCTCTAATCTTGTTCTTCACGGTGATATTCCACAAGATTTTGAAATGGAATCAAAAGGGCAGTGGTTTC
  ACATCAGCGACACACATGTGCAAGCTGTGCCTACAACTAAAGTACTAAACTCACAAGCGTACCTCCTATT
  TTATGAGAGAATACTGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001001992
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001992.1, NP_001001992.1
• RefSeq Size: 2986 bp
• RefSeq ORF: 2469 bp
• Locus ID: 10600
• Cytogenetics: 21q21.3
• Protein Families: Protease
• Gene Summary: This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.