CD32B (FCGR2B) (NM_001002273) Human Untagged Clone

CAT#: SC300432

FCGR2B (untagged)-Human Fc fragment of IgG, low affinity IIb, receptor (CD32) (FCGR2B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FCGR2B
• Synonyms: CD32; CD32B; FCG2; FCGR2; FCGR2C; FcRII-c; IGFR2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001002273 edited
  GAATTCGGCACGAGCTGTGCTCTGGGCGCCAGCTCGCTCCAGGGAGTGATGGGAATCCTG
  TCATTCTTACCTGTCCTTGCCACTGAGAGTGACTGGGCTGACTGCAAGTCCCCCCAGCCT
  TGGGGTCATATGCTTCTGTGGACAGCTGTGCTATTCCTGGCTCCTGTTGCTGGGACACCT
  GCTCCCCCAAAGGCTGTGCTGAAACTCGAGCCCCAGTGGATCAACGTGCTCCAGGAGGAC
  TCTGTGACTCTGACATGCCGGGGGACTCACAGCCCTGAGAGCGACTCCATTCAGTGGTTC
  CACAATGGGAATCTCATTCCCACCCACACGCAGCCCAGCTACAGGTTCAAGGCCAACAAC
  AATGACAGCGGGGAGTACACGTGCCAGACTGGCCAGACCAGCCTCAGCGACCCTGTGCAT
  CTGACTGTGCTTTCTGAGTGGCTGGTGCTCCAGACCCCTCACCTGGAGTTCCAGGAGGGA
  GAAACCATCGTGCTGAGGTGCCACAGCTGGAAGGACAAGCCTCTGGTCAAGGTCACATTC
  TTCCAGAATGGAAAATCCAAGAAATTTTCCCGTTCGGATCCCAACTTCTCCATCCCACAA
  GCAAACCACAGTCACAGTGGTGATTACCACTGCACAGGAAACATAGGCTACACGCTGTAC
  TCATCCAAGCCTGTGACCATCACTGTCCAAGCTCCCAGCTCTTCACCGATGGGGATCATT
  GTGGCTGTGGTCACTGGGATTGCTGTAGCGGCCATTGTTGCTGCTGTAGTGGCCTTGATC
  TACTGCAGGAAAAAGCGGATTTCAGCCAATCCCACTAATCCTGATGAGGCTGACAAAGTT
  GGGGCTGAGAACACAATCACCTATTCACTTCTCATGCACCCGGATGCTCTGGAAGAGCCT
  GATGACCAGAACCGTATTTAGTCTCCATTGTCTTGCATTGGGATTTGAGAAGAAAATCAG
  AGAGGGAAGATCTGGTATTTCCTGGCCTAAATTCCCCTTGGGGAGGACAGGGAGATGCTG
  CAGTTCCAAAAGAGAAGGTTTCTTCCAGAGTCATCTACCTGAGTCCTGAAGCTCCCTGTC
  CTGAAAGCCACAGACAATATGGTCCCAAATGACCGACTGCACCTTCTGTGCTTCAGCTCT
  TCTTGACATCAAGGCTCTTCCGTTCCACATCCACACAGCCAATCCAATTAATCAAACCAC
  TGTTATTAACAGATAATAGCAACTTGGGAAATGCTTATGTTACAGGTTACGTGAGAACAA
  TCATGTAAATCTATATGATTTCAGAAATGTTAAAATAGACTAACCTCTACCAGCACATTA
  AAAGTGATTGTTTCTGGGTGATAAAATTATTGATGATTTTTATTTTCTTTATTTTTCTAT
  AAAGATCATATATTACTTTTATAATAAAACATTATAAAAACAAAAAAAAAAAAAAAAAAC
  TCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001002273 unedited
  NNNNCGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGCTGTGC
  TCTGGGCGCCAGCTCGCTCCAGGNAGTGATGGGAATCCTGTCATTCTTACCTGTCCTTGC
  CACTGAGAGTGACTGGGCTGACTGCAAGTCCCCCCAGCCTTGGGGTCATATGCTTCTGTG
  GACAGCTGTGCTATTCCTGGCTCCTGTTGCTGGGACACCTGCTCCCCCAAAGGCTGTGCT
  GAAACTCGAGCCCCAGTGGATCAACGTGCTCCAGGAGGACTCTGTGACTCTGACATGCCG
  GGGGACTCACAGCCCTGAGAGCGACTCCATTCAGTGGTTCCACAATGGGAATCTCATTCC
  CACCCACACGCAGCCCAGCTACAGGTTCAAGGCCAACAACAATGACAGCGGGGAGTACAC
  GTGCCAGACTGGCCAGACCAGCCTCAGCGACCCTGTGCATCTGACTGTGCTTTCTGAGTG
  GCTGGTGCTCCAGACCCCTCACCTGGAGTTCCAGGAGGGAGAAACCATCGTGCTGAGGTG
  CCACAGCTGGAAGGACAAGCCTCTGGTCAAGGTCACATTCTTCCAGAATGGAAAATCCAA
  GAAATTTTCCCGTTCGGATCCCAACTTCTCCATCCCACAAGCAAACCACAGTCACAGTGG
  TGATTACCACTGCACAGGAAACATAGGCTACACGCTGTACTCATTCAAGCCTTGTAACCA
  TCACTGTCCAAGCTCCCAGCTCTTCACCGATGGGNGATCATTGTGGGCTGTGGTCACTGG
  GGATTGCTGTANNCGCCATTGNTGCTGCTGTANTGCCCTTGATCTACTGCAGGAAAAAGC
  GGATTTCAGCCCATCCCACTATNCTGATGAGGCTGACAAAAGTNGGGGGCTGAGAACACA
  TCACCTATTCACTTCTCATGCACCCGGTGCTNCTGGAGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001002273 unedited
  TGACAGNAAAAATAAANAAAATAAAAATCATCAATAATTTTATCACCCAGAAACAATCAC
  TTTTAATGTGCTGGTAGAGGTTAGTCTATTTTAACATTTCTGAAATCATATAGATTTACA
  TGATTGTTCTCACGTAACCTGTAACATAAGCATTTCCCAAGTTGCTATTATCTGTTAATA
  ACAGTGGTTTGATTAATTGGATTGGCTGTGTGGATGTGGAACGGAAGAGCCTTGATGTCA
  AGAAGAGCTGAAGCACAGAAGGTGCAGTCGGTCATTTGGGACCATATTGTCTGTGGCTTT
  CAGGACAGGGAGCTTCAGGACTCAGGTAGATGACTCTGGAAGAAACCTTCTCTTTTGGAA
  CTGCAGCATCTCCCTGTCCTCCCCAAGGGGAATTTAGGCCAGGAAATACCAGATCTTCCC
  TCTCTGATTTTCTTCTCAAATCCCAATGCAAGACAATGGAGACTAAATACGGTTCTGGTC
  ATCAGGCTCTTCCAGAGCATCCGGGTGCATGAGAAGTGAATAGGTGATTGTGTTCTCAGC
  CCCAACTTTGTCAGCCTCATCAGGATTAGTGGGATTGGCTGAAATCCGCTTTTTCCTGCA
  GTAGATCAAGGCCACTACAGCAGCAACAATGGCCGCTACAGCAATCCCAGTGACCACAGC
  CACAATGATCCCCATCGGTGAAGAGCTGGGAGCTTGGACAGTGATGGTCACAGGCTTGGG
  ATGAGTACAGCGTGTAGCCTATGTTTCCTGTGCAGTGGTAATCACCACTGGGACTGTGGC
  TTGGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001002273
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002273.1, NP_001002273.1
• RefSeq Size: 1573 bp
• RefSeq ORF: 873 bp
• Locus ID: 2213
• Cytogenetics: 1q23.3
• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: B cell receptor signaling pathway, Fc gamma R-mediated phagocytosis, Systemic lupus erythematosus
• Gene Summary: 'The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.