MTP18 (MTFP1) (NM_001003704) Human Untagged Clone

CAT#: SC300528

MTFP1 (untagged)-Human mitochondrial fission process 1 (MTFP1), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTFP1
• Synonyms: HSPC242; MTP18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001003704, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCAGAGCCGCAGCCGCGGGGCGCAGAGCGCGATCTCTACCGGGACACGTGGGTGCGATACCTGGGCT
  ATGCCAATGAGGTGGGCGAGGCTTTCCGCTCTCTTGTGCCAGCGGCGGTGGTGTGGCTGAGCTATGGCGT
  GGCCAGCTCCTACGTGCTGGCGGATGCCATTGACAAAGGCAAGAAGGCTGGAGAGGTCGGTGGATTTCCT
  CCTGGACTCCAGCCTGCGCAAGCTCTACCCAACAGTGGGGAAGCCCAGCTCCTCCTGATCATACTCTGGT
  ACCTGGCCTGTGCATCGGCCTCCTGCTTCATGTCAACCTCCTACTCCTGCCAGGGAATGTGGACACCTGG
  CTCCCTGGTGTCCAAAGACCCTGGCACCTGGGTGGGTTTGAGCTGGACAGAAGCTTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003704
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003704.2, NP_001003704.1
• RefSeq Size: 1065 bp
• RefSeq ORF: 408 bp
• Locus ID: 51537
• Cytogenetics: 22q12.2
• Gene Summary: MTP18 is a mitochondrial protein and downstream target of the phosphatidylinositol 3-kinase (see PIK3CA, MIM 171834) signaling pathway that plays a role in cell viability and mitochondrial dynamics (Tondera et al., 2004 [PubMed 15155745]). [supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a.