Plakophilin 2 (PKP2) (NM_001005242) Human Untagged Clone

CAT#: SC300868

PKP2 (untagged)-Human plakophilin 2 (PKP2), transcript variant 2a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PKP2
• Synonyms: ARVD9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001005242, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGCCCCCGGCGCCCCAGCTGAGTACGGCTACATCCGGACCGTCCTGGGCCAGCAGATCCTGGGAC
  AACTGGACAGCTCCAGCCTGGCGCTGCCCTCCGAGGCCAAGCTGAAGCTGGCGGGGAGCAGCGGCCGCGG
  CGGCCAGACAGTCAAGAGCCTGCGGATCCAGGAGCAGGTGCAGCAGACCCTCGCCCGGAAGGGCCGCAGC
  TCCGTGGGCAACGGAAATCTTCACCGAACCAGCAGTGTTCCTGAGTATGTCTACAACCTACACTTGGTTG
  AAAATGATTTTGTTGGAGGCCGTTCCCCTGTTCCTAAAACCTATGACATGCTAAAGGCTGGCACAACTGC
  CACTTATGAAGGTCGCTGGGGAAGAGGAACAGCACAGTACAGCTCCCAGAAGTCCGTGGAAGAAAGGTCC
  TTGAGGCATCCTCTGAGGAGACTGGAGATTTCTCCTGACAGCAGCCCGGAGAGGGCTCACTACACGCACA
  GCGATTACCAGTACAGCCAGAGAAGCCAGGCTGGGCACACCCTGCACCACCAAGAAAGCAGGCGGGCCGC
  CCTCCTAGTGCCACCGAGATATGCTCGTTCCGAGATCGTGGGGGTCAGCCGTGCTGGCACCACAAGCAGG
  CAGCGCCACTTTGACACATACCACAGACAGTACCAGCATGGCTCTGTTAGCGACACCGTTTTTGACAGCA
  TCCCTGCCAACCCGGCCCTGCTCACGTACCCCAGGCCAGGGACCAGCCGCAGCATGGGCAACCTCTTGGA
  GAAGGAGAACTACCTGACGGCAGGGCTCACTGTCGGGCAGGTCAGGCCGCTGGTGCCCCTGCAGCCCGTC
  ACTCAGAACAGGGCTTCCAGGTCCTCCTGGCATCAGAGCTCCTTCCACAGCACCCGCACGCTGAGGGAAG
  CTGGGCCCAGTGTCGCCGTGGATTCCAGCGGGAGGAGAGCGCACTTGACTGTCGGCCAGGCGGCCGCAGG
  GGGAAGTGGGAATCTGCTCACTGAGAGAAGCACTTTCACTGACTCCCAGCTGGGGAATGCAGACATGGAG
  ATGACTCTGGAGCGAGCAGTGAGTATGCTCGAGGCAGACCACATGCTGCCATCCAGGATTTCTGCTGCAG
  CTACTTTCATACAGCACGAGTGCTTCCAGAAATCTGAAGCTCGGAAGAGGGTTAACCAGCTTCGTGGCAT
  CCTCAAGCTTCTGCAGCTCCTAAAAGTTCAGAATGAAGACGTTCAGCGAGCTGTGTGTGGGGCCTTGAGA
  AACTTAGTATTTGAAGACAATGACAACAAATTGGAGGTGGCTGAACTAAATGGGGTACCTCGGCTGCTCC
  AGGTGCTGAAGCAAACCAGAGACTTGGAGACTAAAAAACAAATAACAGGTTTGCTGTGGAATTTGTCATC
  TAATGACAAACTCAAGAATCTCATGATAACAGAAGCATTGCTTACGCTGACGGAGAATATCATCATCCCC
  TTTTCTGGGTGGCCTGAAGGAGACTACCCAAAAGCAAATGGTTTGCTCGATTTTGACATATTCTACAACG
  TCACTGGATGCCTAAGAAACATGAGTTCTGCTGGCGCTGATGGGAGAAAAGCGATGAGAAGATGTGACGG
  ACTCATTGACTCACTGGTCCATTATGTCAGAGGAACCATTGCAGATTACCAGCCAGATGACAAGGCCACG
  GAGAATTGTGTGTGCATTCTTCATAACCTCTCCTACCAGCTGGAGGCAGAGCTCCCAGAGAAATATTCCC
  AGAATATCTATATTCAAAACCGGAATATCCAGACTGACAACAACAAAAGTATTGGATGTTTTGGCAGTCG
  AAGCAGGAAAGTAAAAGAGCAATACCAGGACGTGCCGATGCCGGAGGAAAAGAGCAACCCCAAGGGCGTG
  GAGTGGCTGTGGCATTCCATTGTTATAAGGATGTATCTGTCCTTGATCGCCAAAAGTGTCCGCAACTACA
  CACAAGAAGCATCCTTAGGAGCTCTGCAGAACCTCACGGCCGGAAGTGGACCAATGCCGACATCAGTGGC
  TCAGACAGTTGTCCAGAAGGAAAGTGGCCTGCAGCACACCCGAAAGATGCTGCATGTTGGTGACCCAAGT
  GTGAAAAAGACAGCCATCTCGCTGCTGAGGAATCTGTCCCGGAATCTTTCTCTGCAGAATGAAATTGCCA
  AAGAAACTCTCCCTGATTTGGTTTCCATCATTCCTGACACAGTCCCGAGTACTGACCTTCTCATTGAAAC
  TACAGCCTCTGCCTGTTACACATTGAACAACATAATCCAAAACAGTTACCAGAATGCACGCGACCTTCTA
  AACACCGGGGGCATCCAGAAAATTATGGCCATTAGTGCAGGCGATGCCTATGCCTCCAACAAAGCAAGTA
  AAGCTGCTTCCGTCCTTCTGTATTCTCTGTGGGCACACACGGAACTGCATCATGCCTACAAGAAGGCTCA
  GTTTAAGAAGACAGATTTTGTCAACAGCCGGACTGCCAAAGCCTACCACTCCCTTAAAGACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001005242
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005242.2, NP_001005242.2
• RefSeq Size: 4307 bp
• RefSeq ORF: 2514 bp
• Locus ID: 5318
• Cytogenetics: 12p11.21
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC)
• Gene Summary: 'This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This transcript variant (2a) lacks an alternate exon as compared to transcript variant 2b and encodes the shorter isoform (2a).