Dynamin 1 (DNM1) (NM_001005336) Human Untagged Clone

CAT#: SC300909

DNM1 (untagged)-Human dynamin 1 (DNM1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNM1
• Synonyms: DNM; EIEE31
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001005336 edited
  AGTCGGGGCGCGCGGCTGCAGCGGCGGAGCCGGAGTCGGAGCCGGGAGCGCTAGCGGCAG
  CCGGATCGCAGCCTGCGGGGCCAGCCGCAGCCATGGGCAACCGCGGCATGGAAGATCTCA
  TCCCGCTGGTCAACCGGCTGCAAGACGCCTTCTCTGCCATCGGCCAGAACGCGGACCTCG
  ACCTGCCGCAGATCGCTGTGGTGGGCGGCCAGAGCGCCGGCAAGAGCTCGGTGCTCGAGA
  ATTTCGTAGGCAGGGACTTCTTGCCTCGAGGATCTGGCATTGTCACCCGACGTCCCCTGG
  TCTTGCAGCTGGTCAATGCAACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGAAAGA
  AATTCACCGACTTCGAGGAGGTGCGCCTTGAGATCGAGGCCGAGACCGACAGGGTCACCG
  GCACCAAC:AAGGGCATCTCGCCGGTGCCTATC:AACCTCCGCGTCTACTCGCCGCACGT
  GCTGAACCTGACCCTGGTGGACCTGCCCGGAATGACCAAGGTCCCGGTGGGGGACCAACC
  TCCCGACATCGAGTTCCAGATCCGAGACATGCTTATGCAGTTTGTCACCAAGGAGAACTG
  CCTCATCCTGGCCGTGTCCCCCGCCAACTCTGACCTGGCCAATTCTGACGCCCTCGAGGT
  CGCCAAGGAGGTGGACCCCCAGGGCCAGCGCACCATCGGGGTCATCACCAAGCTGGACCT
  GATGGACGAGGGCACAGATGCCCGTGATGTGCTGGAGAACAAGCTGCTCCCCCTGCGCAG
  AGGCTACATTGGAGTGGTGAACCGGAGCCAGAAGGACATTGATGGCAAGAAGGACATTAC
  CGCCGCCTTGGCTGCTGAACGAAAGTTCTTCCTCTCCCATCCATCTTATCGCCACTTGGC
  TGACCGTATGGGCACGCCCTACCTGCAGAAGGTCCTCAATCAGCAACTGACGGACCACAT
  CCGGGACACACTGCCGGGGCTGCGGAACAAGCTGCAGAGCCAGCTACTGTCCATTGAGAA
  GGAGGTGGAGGAATACAAGAACTTCCGCCCTGATGACCCAGCTCGCAAGACCAAGGCCCT
  GCTGCAGATGGTCCAGCAGTTCGCCGTAGACTTTGAGAAGCGCATTGAGGGCTCAGGAGA
  TCAGATCGACACCTACGAACTGTCAGGGGGAGCCCGCATTAACCGAATCTTCCACGAGCG
  CTTCCCTTTCGAGCTGGTCAAGATGGAGTTTGATGAGAAGGAACTCCGAAGGGAGATCAG
  CTATGCTATCAAGAATATCCATGGCATTAGAACGGGGCTGTTTACCCCAGACATGGCCTT
  TGAGACCATTGTGAAAAAGCAGGTGAAGAAGATCCGAGAACCGTGTCTCAAGTGTGTGGA
  CATGGTTATCTCGGAGCTAATCAGCACCGTTAGACAGTGCACCAAGAAGCTCCAGCAGTA
  CCCGCGGCTACGGGAGGAGATGGAGCGCATCGTGACCACCCACATCCGGGAGCGCGAGGG
  CCGCACTAAGGAGCAGGTCATGCTTCTCATCGATATCGAGCTGGCTTACATGAACACCAA
  CCATGAGGACTTCATAGGCTTTGCCAATGCTCAGCAGAGGAGCAACCAGATGAACAAGAA
  GAAGACTTCAGGGAACCAGGATGAGATTCTGGTCATCCGCAAGGGCTGGCTGACTATCAA
  TAATATTGGCATCATGAAAGGGGGCTCCAAGGAGTACTGGTTTGTGCTGACTGCTGAGAA
  TCTGTCCTGGTACAAGGATGATGAGGAGAAAGAGAAGAAATACATGCTGTCTGTGGACAA
  CCTCAAGCTGCGGGACGTGGAGAAGGGCTTTATGTCGAGCAAGCATATCTTTGCCCTCTT
  TAACACGGAGCAGAGGAATGTCTACAAGGATTATCGGCAGCTGGAGCTAGCCTGTGAGAC
  ACAGGAGGAGGTGGACAGCTGGAAGGCCTCCTTCCTGAGGGCTGGCGTGTACCCTGAGCG
  TGTTGGGGACAAAGAGAAAGCCAGCGAGACCGAGGAGAATGGCTCCGACAGCTTCATGCA
  TTCCATGGACCCACAGCTGGAACGGCAAGTGGAGACCATCCGGAATCTTGTGGACTCATA
  CATGGCCATTGTCAACAAGACCGTGAGGGACCTCATGCCCAAGACCATCATGCACCTCAT
  GATTAACAATACCAAGGAGTTCATCTTCTCGGAGCTGCTGGCCAACCTGTACTCGTGTGG
  GGACCAGAACACGCTGATGGAGGAGTCGGCGGAGCAGGCACAGCGGCGCGACGAGATGCT
  GCGCATGTACCACGCACTGAAGGAGGCGCTCAGCATCATCGGCGACATCAACACGACCAC
  CGTCAGCACGCCCATGCCCCCGCCCGTGGACGACTCCTGGCTGCAGGTGCAGAGCGTACC
  GGCCGGACGCAGGTCGCCCACGTCCAGCCCCACGCCGCAGCGCCGAGCCCCCGCCGTGCC
  CCCAGCCCGGCCCGGGTCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCTGGGTCCGCCAT
  GGGGGGGGCGCCCCCCGTGCCCTCCAGGCCGGGGGCTTCCCCTGACCCTTTCGGCCCTCC
  CCCTCAGGTGCCCTCGCGCCCCAACCGCGCCCCGCCCGGGGTCCCCAGAATCACTATCAG
  TGACCCCTGAGGAGCGTCAGCCATGCCGATCGGGTCAGGCAAGTCCATCCCGTCCTGAGA
  GCCCCAGGCCCCCCTTCGACCTCTAAACAGATCCCTCCTCTTCTCGGAGACCTCCCTTTC
  CAAGCCTGCCTGGACGGCTGTTCTGTGACTTGACAGTGGCTCCCCCAGCCCCAAAGCCAG
  CCCCCTTCATCTGTGACTTAATCTGTTGTAGTGGTGAGCTGATACATTCAGGTGTGACCG
  TTGGTGAAAACTTGTGCCCCTTCTGTGGTATGCCCTTGCCCTGTTCTATAAATATCTATA
  AATACTCATATATATACACACCTACACATGGCCAACCGCCTCGCCTCTAGCGCTGGGAAT
  CAGTCACTGTGCTATCCTTGTGGAGTCTTGTGGCCCAACTACCAGAGAACGCTGTCCCCC
  GACATCCCACTCCAAAGTGTGCCACCTCCAGTGAGCCTCCTTGTCATGCCCGGCCTGTGG
  ACAGCCAGCCCCCGCCATCCCTCCCACCCCCTACCAAGCATGGGGGTGCTGTGCAGGCAG
  CCGTGTGGCCTGACAGTTTCTACCAGTCCTGCTGTCCCTCGGCTGAGAATAAAACCCATT
  TCTGGATGAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001005336
• Insert Size: 3200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain 3 SNPs compared with NM_001005336.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005336.1, NP_001005336.1
• RefSeq Size: 3253 bp
• RefSeq ORF: 2556 bp
• Locus ID: 1759
• Cytogenetics: 9q34.11
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: 'This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region that results in a frameshift and an early stop codon, compared to variant 1. The resulting isoform (2), also known as internal form 2 and the short C-terminal form, is shorter and has a distinct C-terminus, compared to isoform 1.