Plakophilin 1 (PKP1) (NM_001005337) Human Untagged Clone

CAT#: SC300910

PKP1 (untagged)-Human plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1), transcript variant 1a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PKP1
• Synonyms: B6P; EDSFS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001005337, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACCACTCGCCGCTCAAGACCGCCTTGGCGTACGAATGCTTCCAGGACCAGGACAACTCCACGTTGG
  CTTTGCCGTCGGACCAAAAGATGAAAACAGGCACGTCTGGCAGGCAGCGCGTGCAGGAGCAGGTGATGAT
  GACCGTCAAGCGGCAGAAGTCCAAGTCTTCCCAGTCGTCCACCCTGAGCCACTCCAATCGAGGTTCCATG
  TATGATGGCTTGGCTGACAATTACAACTATGGGACCACCAGCAGGAGCAGCTACTACTCCAAGTTCCAGG
  CAGGGAATGGCTCATGGGGATATCCGATCTACAATGGAACCCTCAAGCGGGAGCCTGACAACAGGCGCTT
  CAGCTCCTACAGCCAGATGGAGAACTGGAGCCGGCACTACCCCCGGGGCAGCTGTAACACCACCGGCGCA
  GGCAGCGACATCTGCTTCATGCAGAAAATCAAGGCGAGCCGCAGTGAGCCCGACCTCTACTGTGACCCAC
  GGGGCACCCTGCGCAAGGGCACGCTGGGCAGCAAGGGCCAGAAGACCACCCAGAACCGCTACAGCTTTTA
  CAGCACCTGCAGTGGTCAGAAGGCCATAAAGAAGTGCCCTGTGCGCCCGCCCTCTTGTGCCTCCAAGCAG
  GACCCTGTGTATATCCCGCCCATCTCCTGCAACAAGGACCTGTCCTTTGGCCACTCTAGGGCCAGCTCCA
  AGATCTGCAGTGAGGACATCGAGTGCAGTGGGCTGACCATCCCCAAGGCTGTGCAGTACCTGAGCTCCCA
  GGATGAGAAGTACCAGGCCATTGGGGCCTATTACATCCAGCATACCTGCTTCCAGGATGAATCTGCCAAG
  CAACAGGTCTATCAGCTGGGAGGCATCTGCAAGCTGGTGGACCTCCTCCGCAGCCCCAACCAGAACGTCC
  AGCAGGCCGCGGCAGGGGCCCTGCGCAACCTGGTGTTCAGGAGCACCACCAACAAGCTGGAGACCCGGAG
  GCAGAATGGGATCCGCGAGGCAGTCAGCCTCCTGAGGAGAACCGGGAACGCCGAGATCCAGAAGCAGCTG
  ACTGGGCTGCTCTGGAACCTGTCTTCCACTGACGAGCTGAAGGAGGAACTCATTGCCGACGCCCTGCCTG
  TTCTGGCCGACCGCGTCATCATTCCCTTCTCTGGCTGGTGCGATGGCAATAGCAACATGTCCCGGGAAGT
  GGTGGACCCTGAGGTCTTCTTCAATGCCACAGGCTGCTTGAGGAACCTGAGCTCGGCCGATGCAGGCCGC
  CAGACCATGCGTAACTACTCAGGGCTCATTGATTCCCTCATGGCCTATGTCCAGAACTGTGTAGCGGCCA
  GCCGCTGTGACGACAAGTCTGTGGAAAACTGCATGTGTGTTCTGCACAACCTCTCCTACCGCCTGGACGC
  CGAGGTGCCCACCCGCTACCGCCAGCTGGAGTATAACGCCCGCAACGCCTACACCGAGAAGTCCTCCACT
  GGCTGCTTCAGCAACAAGAGCGACAAGATGATGAACAACAACTATGACTGCCCCCTGCCTGAGGAAGAGA
  CCAACCCCAAGGGCAGCGGCTGGTTGTACCATTCAGATGCCATCCGCACCTACCTGAACCTCATGGGCAA
  GAGCAAGAAAGATGCTACCCTGGAGGCCTGTGCTGGTGCCCTGCAGAACCTGACAGCCAGCAAGGGGCTG
  ATGTCCAGTGGCATGAGCCAGTTGATTGGGCTGAAGGAAAAGGGCCTGCCACAAATTGCCCGCCTCCTGC
  AATCTGGCAACTCTGATGTGGTGCGGTCCGGAGCCTCCCTCCTGAGCAACATGTCCCGCCACCCTCTGCT
  GCACAGAGTGATGGGGAACCAGGTGTTCCCGGAGGTGACCAGGCTCCTCACCAGCCACACTGGCAATACC
  AGCAACTCCGAAGACATCTTGTCCTCGGCCTGCTACACTGTGAGGAACCTGATGGCCTCGCAGCCACAAC
  TGGCCAAGCAGTACTTCTCCAGCAGCATGCTCAACAACATCATCAACCTGTGCCGAAGCAGTGCCTCACC
  CAAGGCCGCAGAAGCTGCCCGGCTTCTCCTGTCTGACATGTGGTCCAGCAAGGAACTGCAGGGTGTCCTC
  AGACAGCAAGGTTTCGATAGGAACATGCTGGGAACCTTAGCTGGGGCCAACAGCCTCAGGAACTTCACCT
  CCCGATTCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001005337
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005337.2, NP_001005337.1
• RefSeq Size: 5384 bp
• RefSeq ORF: 2181 bp
• Locus ID: 5317
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]'
  Transcript Variant: This variant (1a) represents the shorter transcript variant as compared to variant 1b. It encodes the shorter protein (isoform 1a), compared to isoform 1b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.