CACNA2D2 (NM_001005505) Human Untagged Clone

CAT#: SC300988

CACNA2D2 (untagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 2 (CACNA2D2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CACNA2D2
• Synonyms: CACNA2D; CASVDD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001005505 edited
  CATCTTGAATGGAAACATGGCGGTGCCGGCTCGGACCTGCGGCGCCTCTCGGCCCGGCCC
  AGCGCGGACTGCGCGCCCCTGGCCCGGCTGCGGCCCCCACCCTGGCCCCGGCACCCGGCG
  CCCGACGTCCGGGCCCCCGCGCCCGCTGTGGCTGCTGCTGCCGCTTCTACCGCTGCTCGC
  CGCCCCCGGCGCCTCTGCCTACAGCTTCCCCCAGCAGCACACGATGCAGCACTGGGCCCG
  GCGTCTGGAGCAGGAGGTCGACGGCGTGATGCGGATTTTTGGAGGCGTCCAGCAGCTCCG
  TGAGATTTACAAGGACAACCGGAACCTGTTCGAGGTACAGGAGAATGAGCCTCAGAAGTT
  GGTGGAGAAGGTGGCAGGGGACATTGAGAGCCTTCTGGACAGGAAGGTGCAGGCCCTGAA
  GAGACTGGCTGATGCTGCAGAGAACTTCCAGAAAGCACACCGCTGGCAGGACAACATCAA
  GGAGGAAGACATCGTGTACTATGACGCCAAGGCTGACGCTGAGCTGGACGACCCTGAGAG
  TGAGGATGTGGAAAGGGGGTCTAAGGCCAGCACCCTAAGGCTGGACTTCATCGAGGACCC
  AAACTTCAAGAACAAGGTCAACTATTCATACGCGGCTGTACAGATCCCTACGGACATCTA
  CAAAGGCTCCACTGTCATCCTCAATGAGCTCAACTGGACAGAGGCCCTGGAGAATGTGTT
  CATGGAAAACCGCAGACAAGACCCCACACTGCTGTGGCAGGTCTTCGGCAGCGCCACAGG
  AGTCACTCGCTACTACCCGGCCACCCCGTGGCGAGCCCCCAAGAAGATCGACCTGTACGA
  TGTCCGAAGGAGACCCTGGTATATCCAGGGGGCCTCGTCACCCAAAGACATGGTCATCAT
  CGTGGATGTGAGTGGCAGTGTGAGCGGCCTGACCCTGAAGCTGATGAAGACATCTGTCTG
  CGAGATGCTGGACACGCTGTCTGATGATGACTATGTGAATGTGGCCTCGTTCAACGAGAA
  GGCACAGCCTGTGTCATGCTTCACACACCTGGTGCAGGCCAATGTGCGCAACAAGAAGGT
  GTTCAAGGAAGCTGTGCAGGGCATGGTGGCCAAGGGCACCACAGGCTACAAGGCCGGCTT
  TGAGTATGCCTTTGACCAGCTGCAGAACTCCAACATCACTCGGGCCAACTGCAACAAGAT
  GATCATGATGTTCACGGATGGTGGTGAGGACCGCGTGCAGGACGTCTTTGAGAAGTACAA
  TTGGCCAAACCGGACGGTGCGCGTGTTTACTTTCTCCGTGGGGCAGCATAACTATGACGT
  CACACCGCTGCAGTGGATGGCCTGTGCCAACAAAGGCTACTATTTTGAGATCCCTTCCAT
  CGGAGCCATCCGCATCAACACACAGGAATATCTAGATGTGTTGGGCAGGCCCATGGTGCT
  GGCAGGCAAGGAGGCCAAGCAGGTGCAGTGGACCAACGTGTATGAGGATGCACTGGGACT
  GGGGTTGGTGGTAACAGGGACCCTCCCTGTTTTCAACCTGACACAGGATGGCCCTGGGGA
  AAAGAAGAACCAGCTGATCCTGGGCGTGATGGGCATTGACGTGGCTCTGAATGACATCAA
  GAGGCTGACCCCCAACTACACGCTTGGAGCCAACGGCTATGTGTTTGCCATTGACCTGAA
  CGGCTACGTGTTGCTGCACCCCAATCTCAAGCCCCAGACCACCAACTTCCGGGAGCCTGT
  GACTCTGGACTTCCTGGATGCGGAGCTAGAGGATGAGAACAAGGAAGAGATCCGTCGGAG
  CATGATTGATGGCAACAAGGGCCACAAGCAGATCAGAACGTTGGTCAAGTCCCTGGATGA
  GAGGTACATAGATGAGGTGACACGGAACTACACCTGGGTGCCTATAAGGAGCACTAACTA
  CAGCCTGGGGCTGGTGCTCCCACCCTACAGCACCTTCTACCTCCAAGCCAATCTCAGTGA
  CCAGATCCTGCAGGTCAAGTATTTTGAGTTCCTGCTCCCCAGCAGCTTTGAGTCTGAAGG
  ACACGTTTTCATTGCTCCCAGAGAGTACTGCAAGGACCTGAATGCCTCAGACAACAACAC
  CGAGTTCCTGAAAAACTTTATTGAGCTCATGGAGAAAGTGACTCCAGACTCCAAGCAGTG
  CAACAACTTCCTTCTGCACAACCTGATCTTGGACACGGGCATCACGCAGCAGCTGGTAGA
  GCGTGTGTGGAGGGACCAGGATCTCAACACGTACAGCCTACTGGCCGTGTTCGCTGCCAC
  AGACGGTGGCATCACCCGAGTCTTCCCCAACAAGGCAGCTGAGGACTGGACAGAGAACCC
  TGAGCCCTTCAATGCCAGCTTCTACCGCCGCAGCCTGGATAACCACGGTTATGTCTTCAA
  GCCCCCACACCAGGATGCCCTGTTAAGGCCGCTGGAGCTGGAGAATGACACTGTGGGCAT
  CCTCGTCAGCACAGCTGTGGAGCTCAGCCTAGGCAGGCGCACACTGAGGCCAGCAGTGGT
  GGGCGTCAAGCTGGACCTAGAGGCTTGGGCTGAGAAGTTCAAGGTGCTAGCCAGCAACCG
  TACCCACCAAGACCAGCCTCAGAAGTGCGGCCCCAACAGCCACTGTGAGATGGACTGCGA
  GGTTAACAATGAGGACTTACTCTGTGTCCTCATTGATGATGGAGGATTCCTGGTGCTGTC
  AAACCAGAACCATCAGTGGGACCAGGTGGGCAGGTTCTTCAGTGAGGTGGATGCCAACCT
  GATGCTGGCACTCTACAATAACTCCTTCTACACCCGCAAGGAGTCCTATGACTATCAGGC
  AGCCTGTGCCCCTCAGCCCCCTGGCAACCTGGGTGCTGCACCCCGGGGTGTCTTTGTGCC
  CACCGTTGCAGATTTCCTTAACCTGGCCTGGTGGACCTCTGCTGCCGCCTGGTCCCTGTT
  CCAGCAGCTTCTCTACGGCCTCATCTACCACAGCTGGTTCCAAGCAGACCCCGCGGAGGC
  CGAGGGGAGCCCCGAGACACGCGAGAGCAGCTGCGTCATGAAACAGACCCAGTACTACTT
  CGGCTCGGTAAACGCCTCCTACAACGCCATCATCGACTGCGGAAACTGCTCCAGGCTGTT
  CCACGCGCAGAGACTGACCAACACCAATCTTCTCTTTGTGGTGGCCGAGAAGCCGCTGTG
  CAGCCAGTGCGAGGCTGGCCGGCTGCTGCAGAAGGAGACGCACTGCCCAGCGGACGGCCC
  GGAGCAGTGTGAGCTAGTGCAGAGACCGCGATACCGGAGAGGCCCGCACATCTGCTTCGA
  CTACAACGCGACAGAAGATACCTCAGACTGTGGCCGCGGGGCCTCCTTCCCGCCGTCGCT
  GGGCGTCCTGGTCTCCCTGCAACTGCTGCTCCTCCTGGGCCTGCCGCCCCGGCCGCAGCC
  TCAAGTCCTCGTCCACGCCTCTCGCCGCCTCTGAGCACCCTGCCCCACCCCACCTCCACT
  CCCACCTCACCCGGCCTCTTCGCCTTTCCCACCCTCCTGCCCCACACTCCCCGCCTTAGA
  GCCTCGTCCCTCCCTCACTGAAGGACCTGAGCTGGCCAGGCCCTGAGAGTCTGGTCTGCG
  CCTTGGGATGGGGAGTCCCAAAGCGGGACGCCGCAGGTGTTTGGCACCCAAATCACATCT
  CACCTCCGAACTGTTCAAGTGTCCCCAGACCCTTCTTGCCTGCTGGGCTCCCCCCAGTGG
  GATGGGACAGGGAGGCCACACGCACTGGTGCCAAAACCAGGCCTCTGCTGCCGCCCTTCC
  TGGAGGCTGCCTATGTTGGGGGGGACCCTGCCTCAGCTGACCCGGCCTCTCTGCCCCACC
  CAAGCCCAAACTTGGTTTCTGTGAGAATAGTGGAGGAAGGTGAGATGGCCAGTTTGAAGC
  CTGTGCCTCCCAGCTTAAATCCTAGCAGGAGAGAGGCTCTGGGGCAGCCCCCATGGGCTC
  CTGCCCCTTTCAGGCCTACAGCCACATCCCCAAGCCCACCAGGTGTCAGGATAGTCACAG
  TGATACCAGTTCAGACACTACCCCATATACACCTGGAACATTGAGGATGGAAACTGGACT
  CACATTCGACATACCCCACTGGGCACACGCACAAACACACACACTATGGGGTGGGGTGGG
  TGTAGGGGCTTACAAAGCCTTACACAGGGCGAGGGGTTGGTGGGAGGGTTGGCACCTGCA
  CACTCCATCTCCTGCTCACCACCTGCCTCTAATCTGAGCTGCAGCCTGGCTGGTCCTCCC
  ATTTCTAAAGCTGAATGTCAAACAGTGCCAAATGCTGGGGCAGGGGGTGAAGAACCCTCT
  GTCCCACCCCTAGCCACCAGTGTCCTCCAAGTGCCCCCTCACCTCTCCAGGTGCTCATTG
  TAACCATTTCTCACTAGTGTCAGGCCCCCAGTGGGACCACATGCCACTGCCTGCACCTTT
  CGGCAGAGGAACCCCCACCAGACATCACCCTTTGCCTTAGCAGGGGTGACTTTGTCTCTC
  CTGGCTGGGCCATCCTTCCGCCAATCTGGCCCTTACACACTCAGGCCTGTGCCCACTCCC
  TATCTCCTTCCCACCCCTACACACACACTCCCTGCTTGCAGGAGGCCAAACTGTCCCTCC
  CTTGCTGAACACACACACACACACACACACACAGGTGGGGACTGGGCACAGCTCTTCACA
  CCATTCATTCTGGTCATTTCCCCCAAAGGCATCCCAGCCTGGGGGCCAGTGGGGAACTGA
  GGGCAAGGGGATATAGTGATGGGGCTCAGATGGACTGGGAGGAGGGGGAGGGTGATGCAT
  TAATTAATGGCTTCGTTAATTAATGTCATGTTGCTTGTCGCTTTCTCAGTGTGTGTGTGT
  GGTCCATGCCCACTGCTGGTGCCAGGGTGGGTGTCCATGTGCACCCGGCCTGGATGCCAG
  CTGTGTCCTTCGGGGGCGTGCGTGTAACTGTAGTGTAGTCAGGTGCTCAATGGAGAATAT
  AAACATATACAGAAAAATATATATTTTAAGTTTAAAAAACAGAAAAACAGACAAAACAAT
  CCCCATCAGGTAGCTGTCTAACCCCCAGCTGGGTCTAATCCTTCTCATTACCCACCCGAC
  CTGGCTGCCCCTCACCTTGGGCTGGGGGACTGGGGGGCCATTTCCTTTTCTCTGCCCTTT
  TTTTGTTGTTCTATTTTGTACAGACAAGTTGGAAAAACAACAGCGACAAAAAAGTCAAGA
  AACTTTGTAAAATATAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001005505 unedited
  TGACAGGTTCGGATTTGTAATACCACTTACTATAGGGCGGCCGCGCATTCGCCCTTCCGC
  ATCTTGAAGGAAACATGGCGGTGCCGGCTCGGACCTGCGGCGCCTCTCGGCCCGGCCCAG
  CGCGGACTGCGCGCCCCTGGCCCGGCTGCGGCCCCCACCCTGGCCCCGGCACCCGGCGCC
  CGACGTCCGGGCCCCCGCGCCCGCTGTGGCTGCTGCTGCCGCTTCTACCGCTGCTCGCCG
  CCCCCGGCGCCTCTGCCTACAGCTTCCCCCAGCAGCACACGATGCAGCACTGGGCCCGGC
  GTCTGGAGCAGGAGGTCGACGGCGTGATGCGGATTTTTGGAGGCGTCCAGCAGCTCCGTG
  AGATTTACAAGGACAACCGGAACCTGTTCGAGGTACAGGAGAATGAGCCTCAGAAGTTGG
  TGGAGAAGGTGGCAGGGGACATTGAGAGCCTTCTGGACAGGAAGGTGCAGGCCCTGAAGA
  GACTGGCTGATGCTGCAGAGAACTTCCAGAAAGCACACCGCTGGCAGGACAACATCAAGG
  AGGAAGACATCGTGTACTATGACGCCAAGGCTGACGCTGAGCTGGACGACCCTGAGAGTG
  AGGATGTGGAAAGGGGGTCTAAGGCCAGCACCCTAAGGCTGGACTTCATCGAGGACCCAA
  ACTTCAAGAACAGGTCAACTATTCATACGCGGCTGTACAGATCCCTACCGACATCTACAA
  AGGCTCCACTGTCATCCTCAATGAGCTCAACTGGACAGAGGCCCTGGAGAATGTGTTCAT
  GGAAAACCGCAGACAAGACCCCACACTGCTGTGGCAGGTCTTCGGCAGCGCCACAAGAGT
  CACTCGCTACTACCCGGCCACCCCGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_001005505 unedited
  CTCGGAATGGCACTTCCAGGACCGGAAAAGCACTGGGGAAGGGTCACAGGATGCCACCCG
  GATCTGTTCAGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT
  TTTTTTTTATATTTTACAAAGTTTCTTGACTTTTTTGTCGCTGTTGTTTTTCCAACTTGT
  CTGTACAAAATACAACAACAAAAAAGGGCAGAGAAAAGGAAATGGCCCCCCAGTCCCCCA
  GCCCAAGGTGAGGGGCAGCCAGGTCGGGTGGGTAATGAGAAGGATTACACCCAGCTGGGG
  GTTAAACAGCTACCTGATGGGGATTGTTTTGTCTGTTTTTCTGTTTTTTAAACTTAAAAT
  ATATATTTTTCTGTATATGTTTATATTCTCCATTGAGCACCTGACTACACTACAGTTACA
  CGCACGCCCCCGAAGGACACAGCTGGCATCCAGGCCGGGTGCACATGGACACCCACCCTG
  GCACCAGCAGTGGGCATGGACCACACACACACACTGAGAAAGCGACTAGCAACATGACAT
  TAATTAACGAAGCCATTAATTAATGCATCACCCTCCCCCTCCTCCCAGTCCATCTGAGCC
  CCATCACTATATCACCTTGCCCTCAGTTCCCACTGGCCCTCAGGCTGGGATGCCTTTTGG
  GGAAATGACCAGAATGAATGGTGTGAANAGCTTGCCCAGTCCCCACCTGTGTGTGCGTGT
  GTGTGCGTGTGTTCACCAAGGGAGGACAGTTTTGGCCTCCTGCAACCAGGGATTGTGTGT
  TTAGGGGTGGGAAGAAAAAGGGAATGGGCACAGCCCTGGATGTGTAAGGGCCAAAATTGG
  CGCAAGATTGGCCCAACCAGGAAAAACC
  
• Restriction Sites: Please inquire
• ACCN: NM_001005505
• Insert Size: 5300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005505.1, NP_001005505.1
• RefSeq Size: 5343 bp
• RefSeq ORF: 3438 bp
• Locus ID: 9254
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• Gene Summary: Calcium channels mediate the entry of calcium ions into the cell upon membrane polarization. This gene encodes the alpha-2/delta subunit of the voltage-dependent calcium channel complex. The complex consists of the main channel-forming subunit alpha-1, and auxiliary subunits alpha-2/delta, beta, and gamma. The auxiliary subunits function in the assembly and membrane localization of the complex, and modulate calcium currents and channel activation/inactivation kinetics. The subunit encoded by this gene undergoes post-translational cleavage to yield the extracellular alpha2 peptide and a membrane-anchored delta polypeptide. This subunit is a receptor for the antiepileptic drug, gabapentin. Mutations in this gene are associated with early infantile epileptic encephalopathy. Single nucleotide polymorphisms in this gene are correlated with increased sensitivity to opioid drugs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (1) lacks an alternate in-frame exon in the central coding region and uses an alternate in-frame splice site in the 3' coding region compared to variant 3. The encoded isoform (a) is shorter than isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.