NUMB (NM_001005743) Human Untagged Clone

CAT#: SC301022

NUMB (untagged)-Human numb homolog (Drosophila) (NUMB), transcript variant 1


  "NM_001005743" in other vectors (6)

Reconstitution Protocol

USD 1,100.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NUMB
Synonyms C14orf41; c14_5527; S171
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF Sequence for NM_001005743. The custom clone sequence may differ by one or more nucleotides
ATGAACAAATTACGGCAAAGTTTTAGGAGAAAGAAGGATGTTTATGTTCCAGAGGCCAGT
CGTCCACATCAGTGGCAGACAGATGAAGAAGGCGTTCGCACCGGAAAATGTAGCTTCCCG
GTTAAGTACCTTGGCCATGTAGAAGTTGATGAATCAAGAGGAATGCACATCTGTGAAGAT
GCTGTAAAAAGATTGAAAGCTGAAAGGAAGTTCTTCAAAGGCTTCTTTGGAAAAACTGGA
AAGAAAGCAGTTAAAGCAGTTCTGTGGGTCTCAGCAGATGGACTCAGAGTTGTGGATGAA
AAAACTAAGGACCTCATAGTTGACCAGACGATAGAGAAAGTTTCTTTCTGTGCCCCAGAC
AGGAACTTTGATAGAGCCTTTTCTTACATATGCCGTGATGGCACCACTCGTCGCTGGATC
TGTCACTGCTTCATGGCTGTCAAGGACACAGGTGAAAGGTTGAGCCATGCAGTAGGCTGT
GCTTTTGCAGCCTGTTTAGAGCGCAAGCAGAAGCGGGAGAAGGAATGTGGAGTGACTGCT
ACTTTTGATGCTAGTCGGACCACTTTTACAAGAGAAGGATCATTCCGTGTCACAACAGCC
ACTGAACAAGCAGAAAGAGAGGAGATCATGAAACAAATGCAAGATGCCAAGAAAGCTGAA
ACAGATAAGATAGTCGTTGGTTCATCAGTTGCCCCTGGCAACACTGCCCCATCCCCATCC
TCTCCCACCTCTCCTACTTCTGATGCCACGACCTCTCTGGAGATGAACAATCCTCATGCC
ATCCCACGCCGGCATGCTCCAATTGAACAGCTTGCTCGCCAAGGCTCTTTCCGAGGTTTT
CCTGCTCTTAGCCAGAAGATGTCACCCTTTAAACGCCAACTATCCCTACGCATCAATGAG
TTGCCTTCCACTATGCAGAGGAAGACTGATTTCCCCATTAAAAATGCAGTGCCAGAAGTA
GAAGGGGAGGCAGAGAGCATCAGCTCCCTGTGCTCACAGATCACCAATGCCTTCAGCACA
CCTGAGGACCCCTTCTCATCTGCTCCGATGACCAAACCAGTGACAGTGGTGGCACCACAA
TCTCCTACCTTCCAAGCTAATGGCACTGACTCAGCCTTCCATGTGCTTGCTAAGCCAGCC
CATACTGCTCTAGCACCCGTAGCAATGCCTGTGCGTGAAACCAACCCTTGGGCCCATGCC
CCTGATGCTGCTAACAAGGAAATTGCAGCCACATGTTCGGGGACCGAGTGGGGTCAATCT
TCTGGTGCTGCCTCTCCAGGTCTCTTCCAGGCCGGTCATAGACGTACTCCCTCTGAGGCC
GACCGATGGTTAGAAGAGGTGTCTAAGAGCGTCCGGGCTCAGCAGCCCCAGGCCTCAGCT
GCTCCTCTGCAGCCAGTTCTCCAGCCTCCTCCACCCACTGCCATCTCCCAGCCAGCATCA
CCTTTCCAAGGGAATGCATTCCTCACCTCTCAGCCTGTGCCAGTGGGTGTGGTCCCAGCC
CTGCAACCAGCCTTTGTCCCTGCCCAGTCCTATCCTGTGGCCAATGGAATGCCCTATCCA
GCCCCTAATGTGCCTGTGGTGGGCATCACTCCCTCCCAGATGGTGGCCAACGTATTTGGC
ACTGCAGGCCACCCTCAGGCTGCCCATCCCCATCAGTCACCCAGCCTGGTCAGGCAGCAG
ACATTCCCTCACTACGAGGCAAGCAGTGCTACCACCAGTCCCTTCTTTAAGCCTCCTGCT
CAGCACCTCAACGGTTCTGCAGCTTTCAATGGTGTAGATGATGGCAGGTTGGCCTCAGCA
GACAGGCATACAGAGGTTCCTACAGGCACCTGCCCAGTGGATCCTTTTGAAGCCCAGTGG
GCTGCATTAGAAAATAAGTCCAAGCAGCGTACTAATCCCTCCCCTACCAACCCTTTCTCC
AGTGACTTACAGAAGACGTTTGAAATTGAACTT
Restriction Sites NotI-NotI     
ACCN NM_001005743
Insert Size 2700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001005743.1, NP_001005743.1
RefSeq Size 3647 bp
RefSeq ORF 1956 bp
Locus ID 8650
Cytogenetics 14q24.2-q24.3
Protein Pathways Notch signaling pathway
Gene Summary The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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