NUMB (NM_001005744) Human Untagged Clone

CAT#: SC301023

NUMB (untagged)-Human numb homolog (Drosophila) (NUMB), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NUMB
• Synonyms: C14orf41; c14_5527; S171
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001005744 edited
  GGCCACTGGCGGCCGAGGTAGAGGCAGTGGCGCTTGAGTTGGTCGGGGGCAGCGGCAGAT
  TTGAGGCTTAAGCAACTTCTTCCGGGGAAGAGTGCCAGTGCAGCCACTGTTACAATTCAA
  GATCTTGATCTATATCCATAGATTGGAATATTGGTGGGCCAGCAATCCTCAGACGCCTCA
  CTTAGGACAAATGAGGAAACTGAGGCTTGGTGAAGTTACGAAACTTGTCCAAAATCACAC
  AACTTGTAAAGGGCACAGCCAAGATTCAGAGCCAGGCTGTAAAAATTAAAATGAACAAAT
  TACGGCAAAGTTTTAGGAGAAAGAAGGATGTTTATGTTCCAGAGGCCAGTCGTCCACATC
  AGTGGCAGACAGATGAAGAAGGCGTTCGCACCGGAAAATGTAGCTTCCCGGTTAAGTACC
  TTGGCCATGTAGAAGTTGATGAATCAAGAGGAATGCACATCTGTGAAGATGCTGTAAAAA
  GATTGAAAGCTGAAAGGAAGTTCTTCAAAGGCTTCTTTGGAAAAACTGGAAAGAAAGCAG
  TTAAAGCAGTTCTGTGGGTCTCAGCAGATGGACTCAGAGTTGTGGATGAAAAAACTAAGG
  ACCTCATAGTTGACCAGACGATAGAGAAAGTTTCTTTCTGTGCCCCAGACAGGAACTTTG
  ATAGAGCCTTTTCTTACATATGCCGTGATGGCACCACTCGTCGCTGGATCTGTCACTGCT
  TCATGGCTGTCAAGGACACAGGTGAAAGGTTGAGCCATGCAGTAGGCTGTGCTTTTGCAG
  CCTGTTTAGAGCGCAAGCAGAAGCGGGAGAAGGAATGTGGAGTGACTGCTACTTTTGATG
  CTAGTCGGACCACTTTTACAAGAGAAGGATCATTCCGTGTCACAACAGCCACTGAACAAG
  CAGAAAGAGAGGAGATCATGAAACAAATGCAAGATGCCAAGAAAGCTGAAACAGATAAGA
  TAGTCGTTGGTTCATCAGTTGCCCCTGGCAACACTGCCCCATCCCCATCCTCTCCCACCT
  CTCCTACTTCTGATGCCACGACCTCTCTGGAGATGAACAATCCTCATGCCATCCCACGCC
  GGCATGCTCCAATTGAACAGCTTGCTCGCCAAGGCTCTTTCCGAGGTTTTCCTGCTCTTA
  GCCAGAAGATGTCACCCTTTAAACGCCAACTATCCCTACGCATCAATGAGTTGCCTTCCA
  CTATGCAGAGGAAGACTGATTTCCCCATTAAAAATGCAGTGCCAGAAGTAGAAGGGGAGG
  CAGAGAGCATCAGCTCCCTGTGCTCACAGATCACCAATGCCTTCAGCACACCTGAGGACC
  CCTTCTCATCTGCTCCGATGACCAAACCAGTGACAGTGGTGGCACCACAATCTCCTACCT
  TCCAAGGGACCGAGTGGGGTCAATCTTCTGGTGCTGCCTCTCCAGGTCTCTTCCAGGCCG
  GTCATAGACGTACTCCCTCTGAGGCCGACCGATGGTTAGAAGAGGTGTCTAAGAGCGTCC
  GGGCTCAGCAGCCCCAGGCCTCAGCTGCTCCTCTGCAGCCAGTTCTCCAGCCTCCTCCAC
  CCACTGCCATCTCCCAGCCAGCATCACCTTTCCAAGGGAATGCATTCCTCACCTCTCAGC
  CTGTGCCAGTGGGTGTGGTCCCAGCCCTGCAACCAGCCTTTGTCCCTGCCCAGTCCTATC
  CTGTGGCCAATGGAATGCCCTATCCAGCCCCTAATGTGCCTGTGGTGGGCATCACTCCCT
  CCCAGATGGTGGCCAACGTATTTGGCACTGCAGGCCACCCTCAGGCTGCCCATCCCCATC
  AGTCACCCAGCCTGGTCAGGCAGCAGACATTCCCTCACTACGAGGCAAGCAGTGCTACCA
  CCAGTCCCTTCTTTAAGCCTCCTGCTCAGCACCTCAACGGTTCTGCAGCTTTCAATGGTG
  TAGATGATGGCAGGTTGGCCTCAGCAGACAGGCATACAGAGGTTCCTACAGGCACCTGCC
  CAGTGGATCCTTTTGAAGCCCAGTGGGCTGCATTAGAAAATAAGTCCAAGCAGCGTACTA
  ATCCCTCCCCTACCAACCCTTTCTCCAGTGACTTACAGAAGACGTTTGAAATTGAACTTT
  AAGCAATCATTATGGCTATGTATCTTGTCCATACCAGACAGGGAGCAGGGGGTAGCGGTC
  AAAGGAGCAAAACAGACTTTGTCTCCTGATTAGTACTCTTTTCACTAATCCCAAAGGTCC
  CAAGGAACAAGTCCAGGCCCAGAGTACTGTGAGGGGTGATTTTGAAAGACATGGGAAAAA
  GCATTCCTAGAGAAAAGCTGCCTTGCAATTAGGCTAAAGAAGTCAAGGAAATGTTGCTTT
  CTGTACTCCCTCTTCCCTTACCCCCTTACAAATCTCTGGCAACAGAGAGGCAAAGTATCT
  GAACAAGAATCTATATTCCAAGCACATTTACTGAAATGTAAAACACAACAGGAAGCAAAG
  CAATCTCCCTTTGTTTTTCAGGCCATTCACCTGCCTCCTGTCAGTAGTGGCCTGTATTAG
  AGATCAAGAAGAGTGGTTTGTGCTCAGGCTGGGGAACAGAGAGGCACGCTATGCTGCCAG
  AATTCCCAGGAGGGCATATCAGCAACTGCCCAGCAGAGCTATATTTTGGGGGAGAAGTTG
  AGCTTCCATTTTGAGTAACAGAATAAATATTATATATATCAAAAGCCAAAATCTTTATTT
  TTATGCATTTAGAATATTTTAAATAGTTCTCAGATATTAAGAAGTTGTATGAGTTGTAAG
  TAATCTTGCCAAAGGTAAAGGGGCTAGTTGTAAGAAATTGTACATAAGATTGATTTATCA
  TTGATGCCTACTGAAATAAAAAGAGGAAAGGCTGGAAGCTGCAGACAGGATCCCTAGCTT
  GTTTTCTGTCAGTCATTCATTGTAAGTAGCACATTGCAACAACAATCATGCTTATGACCA
  ATACAGTCACTAGGTTGTAGTTTTTTTTAAATAAAGGAAAAGCAGTATTGTCCTGGTTTT
  AAACCTATGATGGAATTCTAATGTCATTATTTTAATGGAATCAATCGAAATATGCTCTAT
  AGAGAATATATCTTTTATATATTGCTGCAGTTTCCTTATGTTAATCCTTTAACACTAAGG
  TAACATGACATAATCATACCATAGAAGGGAACACAGGTTACCATATTGGTTTGTAATATG
  GGTCTTGGTGGGTTTTGTTTTATCCTTTAAATTTTGTTCCCATGAGTTTTGTGGGGATGG
  GGATTCTGGTTTTATTAGCTTTGTGTGTGTCCTCTTCCCCCAAACCCCCTTTTGGTGAGA
  ACATCCCCTTGACAGTTGCAGCCTCTTGACCTCGGATAACAATAAGAGAGCTCATCTCAT
  TTTTACTTTTGAACGTTGGCCTTACAATCAAATGTAAGTTATATATATTTGTACTGATGA
  AAATTTATAATCTGCTTTAACAAAAATAAATGTTCATGGTAGAAAAAAAAAAAAAAAAAA
  AAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001005744
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone is found to be a perfect match to NM_001005744.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005744.1, NP_001005744.1
• RefSeq Size: 3503 bp
• RefSeq ORF: 1812 bp
• Locus ID: 8650
• Cytogenetics: 14q24.2-q24.3
• Protein Pathways: Notch signaling pathway
• Gene Summary: The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Variants 2 and 5 both encode the same protein.