TrkB (NTRK2) (NM_001007097) Human Untagged Clone

CAT#: SC301152

NTRK2 (untagged)-Human neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NTRK2
• Synonyms: EIEE58; GP145-TrkB; OBHD; trk-B; TRKB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001007097 edited
  ATGTCGTCCTGGATAAGGTGGCATGGACCCGCCATGGCGCGGCTCTGGGGCTTCTGCTGG
  CTGGTTGTGGGCTTCTGGAGGGCCGCTTTCGCCTGTCCCACGTCCTGCAAATGCAGTGCC
  TCTCGGATCTGGTGCAGCGACCCTTCTCCTGGCATCGTGGCATTTCCGAGATTGGAGCCT
  AACAGTGTAGATCCTGAGAACATCACCGAAATTTTCATCGCAAACCAGAAAAGGTTAGAA
  ATCATCAACGAAGATGATGTTGAAGCTTATGTGGGACTGAGAAATCTGACAATTGTGGAT
  TCTGGATTAAAATTTGTGGCTCATAAAGCATTTCTGAAAAACAGCAACCTGCAGCACATC
  AATTTTACCCGAAACAAACTGACGAGTTTGTCTAGGAAACATTTCCGTCACCTTGACTTG
  TCTGAACTGATCCTGGTGGGCAATCCATTTACATGCTCCTGTGACATTATGTGGATCAAG
  ACTCTCCAAGAGGCTAAATCCAGTCCAGACACTCAGGATTTGTACTGCCTGAATGAAAGC
  AGCAAGAATATTCCCCTGGCAAACCTGCAGATACCCAATTGTGGTTTGCCATCTGCAAAT
  CTGGCCGCACCTAACCTCACTGTGGAGGAAGGAAAGTCTATCACATTATCCTGTAGTGTG
  GCAGGTGATCCGGTTCCTAATATGTATTGGGATGTTGGTAACCTGGTTTCCAAACATATG
  AATGAAACAAGCCACACACAGGGCTCCTTAAGGATAACTAACATTTCATCCGATGACAGT
  GGGAAGCAGATCTCTTGTGTGGCGGAAAATCTTGTAGGAGAAGATCAAGATTCTGTCAAC
  CTCACTGTGCATTTTGCACCAACTATCACATTTCTCGAATCTCCAACCTCAGACCACCAC
  TGGTGCATTCCATTCACTGTGAAAGGCAACCCCAAACCAGCGCTTCAGTGGTTCTATAAC
  GGGGCAATATTGAATGAGTCCAAATACATCTGTACTAAAATACATGTTACCAATCACACG
  GAGTACCACGGCTGCCTCCAGCTGGATAATCCCACTCACATGAACAATGGGGACTACACT
  CTAATAGCCAAGAATGAGTATGGGAAGGATGAGAAACAGATTTCTGCTCACTTCATGGGC
  TGGCCTGGAATTGACGATGGTGCAAACCCAAATTATCCTGATGTAATTTATGAAGATTAT
  GGAACTGCAGCGAATGACATCGGGGACACCACGAACAGAAGTAATGAAATCCCTTCCACA
  GACGTCACTGATAAAACCGGTCGGGAACATCTCTCGGTCTATGCTGTGGTGGTGATTGCG
  TCTGTGGTGGGATTTTGCCTTTTGGTAATGCTGTTTCTGCTTAAGTTGGCAAGACACTCC
  AAGTTTGGCATGAAAGGTTTTGTTTTGTTTCATAAGATCCCACTGGATGGGTAGCTGAAA
  TAAAGGAAAAGACAGAGAAAGGGGCTGTGGTGCTTGTTGGTTGATGCTGCCATGTAAGCT
  GGACTCCTGGGACTGCTGTTGGCTTATCCCGGGAAGTGCTGCTTATCTGGGGTTTTCTGG
  TAGATGTGGGCGGTGTTTGGAGGCTGTACTATATGAAGCCTGCATATACTGTGAGCTGTG
  ATTGGGGAACACCAATGCAGAGGTAACTCTCAGGCAGCTAAGCAGCACCTCAAGAAAACA
  TGTTAAATTAATGCTTCTCTTCTTACAGTAGTTCAAATACAAAACTGAAATGAAATCCCA
  TTGGATTGTACTTCTCTTCTGAAAAGTGTGCTTTTTGACCCTACTGGACATTTATTGACT
  TAATTGCTTCTGTTTATTAAAATTGACCTGCAAAGTTAAAAAAAAATTAAAGTTGAGAAC
  AGGTATAAGTGCACACTGAATAGTCTAATCTACATGTAACACATATTTTAGTATGATTTT
  CTATACTCTAATCAGCACTGAATTCAGAGGGTTTGACTTTTTCATCTATAACACAGTGAC
  TAAAAGAGTTAAGGGTATATATACCATCAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001007097 unedited
  TCAGTATATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCGCGAT
  TGTAGAAGAGACTGTGGTGTGAATTAGGGACCGGGAGGCGTCGAACGGAGGAACGGTTCA
  TCTTAGAGCCTCGAGGTGCATACCGGACCCCCATTCGCATCTAACAAGGAATCTGCGCCC
  CAGAGAGTCCCGGGAGCGCCGCCGGTCGGTGCCCGGCGCGCCGGGCCATGCAGCGACGGC
  CGCCGCGGAGCTCCGAGCAGCGGTAGCGCCCCCCTGTAAAGCGGTTCGCTATGCCGGGGC
  CACTGTGAACCCTGCCGCCTGCCGGAACACTCTTCGCTCCGGACCAGCTCAGCCTCTGAT
  AAGCTGGACTCGGCACGCCCGCAACAAGCACCGAGGAGTTAAGAGAGCCGCAAGCGCAGG
  GAAGGCCTCCCCGCACGGGTGGGGGAAAGCGGCCGGTGCAGCGCGGGGACAGGCACTCGG
  GCTGGCACTGGCTGCTAGGGATGTCGTCCTGGATAAGGTGGCATGGACCCGCCATGGCGC
  GGCTCTGGGGCTTCTGCTGGCTGGTTGTGGGCTTCTGGAGGGCCGCTTTCGCCTGTCCCA
  CGTCCTGCANATGCAGTGCCTCTCGGATCTGGTGCAGCGACCCTTCTCCTGGCATCGTGG
  CATTTCCGAGATTGGAGCCTAACAGTGTAGATCCCTGAGAACATCACCGAATTTTCATCG
  CANACCAGAAAAGGTTAGAAATCATCAACGAAGATGATGTTTGAGCTTTATGTGGACTGA
  GAAATCTGANCATTGTGGATTCTGGATTAAAATTTGTGGGCTCATAAGCATTTCTGAAAA
  ACAGCAACCTGCAGCACANTCATTTTTACCCCGAACAAACTGACGAGTTTGTCTAGNAAA
  CATTTNCGTCACCTTGACTTTGTCTGACTGATCCCTGTGGGGCATCATTTACATGCTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001007097 unedited
  TGAACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTTTTAAACGTTTT
  TCATTTGTTTGTTTTGTTTTGTTTTGTTTTGCTGGCTAAGGAACTTTAAGACAATCCTTA
  ACATTGTTTGACTCTCAGACCTAAGCTAAACATTTGGACTTTTATTGACAGTGAAGGAAA
  TAACCTTTTAATAATACTACCAAGTCCCAAAGTGATGGTATATATACCCTTAACTCTTTT
  AGTCACTGTGTTATAGATGAAAAAGTCAAACCCTCTGAATTCAGTGCTGATTACAGTATA
  GAAAATCATACTAAAATATGTGTTACATGTAGATTACACTATTCAGTGTGCACTTATACC
  TGTTCTCAACTTTAATTTTTTTTTAACTTTGCAGGTCAATTTTAATAAACAGAAGCAATT
  AAGTCAATAAATGTCCAGTAGGGTCAAAAAAGCACACTTTCCAGAAGAGAAGTACAATCC
  AATGGGATTTCATTTTCACGTTTGTATTTGAACTACTGTAACAATAAAACCATTAATTTA
  ACATGTTTTCTTGAGGTGCTGCTTANCTGCCTGAGAGTTACCTTTGCATTGGTGTTCCCC
  AATAACACCTCACAGTTTATGCACGCTTCATATAGTTCAGGCTTCAAACACCGCCCACTT
  TTACCATAAATACCCCATATAATCACACCTTTTCCGGGATAAATCCAACACTATCTCCAG
  GAGTCCAGTTTTCATGCGCACATCTACTCACCATCTCCCAAGCCCCTTTTTCCCCCCTGT
  TTCTTTATCTAGCCTCCCCCTCCCTGGGGATTCTTTGAACAAAAACCTAACCTTTCCTGC
  CAAACCTCGCACTCCTTCGGCCCCCTTCCGCCAAAACACGCCTTCCACAAAGCGCCTGTT
  CCCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001007097
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007097.1, NP_001007098.1
• RefSeq Size: 7111 bp
• RefSeq ORF: 1434 bp
• Locus ID: 4915
• Cytogenetics: 9q21.33
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: 'This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (b, also known as TrkB-T1) lacks several 3' exons but contains an alternate 3' terminal exon (exon 16 in PMID:11798182), and it thus differs in the 3' coding region and 3' UTR, compared to variant a. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. The 5' UTR is incomplete due to a lack of 5'-complete transcript support for this variant, and because there is ambiguity in the 5' UTR splicing pattern. Variants b and o-w all encode the same isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.