DEPDC5 (NM_001007188) Human Untagged Clone

CAT#: SC301164

DEPDC5 (untagged)-Human DEP domain containing 5 (DEPDC5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DEPDC5
• Synonyms: DEP.5; FFEVF; FFEVF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001007188, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGAACAACAAAGGTCTACAAACTCGTCATCCACAAGAAGGGCTTTGGGGGCAGTGATGATGAGCTAG
  TTGTGAACCCCAAAGTGTTCCCTCACATCAAGCTTGGAGACATTGTAGAGATTGCACACCCCAACGATGA
  ATACAGCCCTCTGCTTTTGCAGGTCAAGTCTCTTAAGGAAGATTTACAGAAGGAAACTATCAGTGTGGAC
  CAGACTGTGACTCAAGTGTTCCGGCTGAGACCTTATCAGGATGTCTATGTTAATGTCGTAGACCCTAAGG
  ATGTGACCCTTGACCTAGTGGAATTAACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGGCGACT
  AAAGAAAAGTTTGGTCAGCACATGTGCCTATATCACCCAGAAGGTGGAGTTTGCTGGCATCAGAGCACAG
  GCTGGTGAACTGTGGGTTAAGAATGAGAAGGTCATGTGTGGCTACATCAGTGAAGATACCAGGGTGGTGT
  TTCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGATTTTGATATTTA
  TGGGGATTTGTATTTTGAGAAAGCTGTGAATGGTTTCCTTGCTGATCTATTTACCAAGTGGAAGGAGAAG
  AACTGTAGTCATGAAGTGACAGTGGTCCTGTTTTCTAGAACTTTCTATGATGCAAAATCTGTTGATGAAT
  TTCCTGAAATAAACCGAGCCTCAATTCGACAGGATCACAAGGGGAGATTCTATGAAGACTTTTACAAAGT
  GGTGGTGCAGAATGAGAGAAGAGAAGAATGGACTTCACTTCTCGTAACCATTAAAAAACTCTTCATCCAG
  TATCCAGTGTTGGTGCGACTGGAACAGGCAGAGGGCTTTCCTCAAGGAGATAATTCTACCTCAGCACAAG
  GAAACTACCTGGAGGCCATCAATCTGTCATTCAATGTGTTTGATAAGCACTACATCAACCGCAACTTTGA
  CCGAACTGGGCAGATGTCAGTGGTGATCACGCCCGGGGTGGGTGTCTTTGAAGTGGACCGCCTACTCATG
  ATCCTGACCAAGCAGCGGATGATAGATAATGGAATTGGTGTGGATTTGGTGTGCATGGGAGAGCAACCGT
  TACATGCTGTCCCATTGTTCAAGCTCCATAATCGGAGTGCTCCCCGTGATTCTCGTCTGGGCGATGACTA
  TAATATCCCTCACTGGATAAACCACAGTTTCTACACATCCAAAAGCCAGCTCTTTTGTAATAGTTTCACC
  CCACGAATAAAACTGGCAGGAAAGAAGCCCGCCTCTGAGAAAGCAAAAAATGGCCGTGATACATCTCTCG
  GGAGTCCAAAAGAATCTGAGAACGCCCTTCCCATCCAAGTAGATTATGACGCCTATGACGCTCAAGTGTT
  CAGGCTGCCCGGCCCATCCCGGGCCCAGTGCCTCACCACCTGCAGATCTGTGCGAGAGCGAGAGAGTCAC
  AGTCGAAAGAGTGCCAGCTCCTGTGATGTTTCATCCAGCCCTTCCCTACCAAGCCGCACACTGCCCACTG
  AGGAAGTGAGGAGCCAGGCTTCTGACGACAGCTCCCTAGGCAAGAGTGCCAACATCCTGATGATCCCACA
  CCCCCACCTGCACCAGTATGAAGTCAGCAGCTCCTTGGGATACACCAGCACTCGAGAGCACCTAGGATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001007188
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007188.2, NP_001007189.1
• RefSeq Size: 2460 bp
• RefSeq ORF: 1680 bp
• Locus ID: 9681
• Cytogenetics: 22q12.2-q12.3
• Gene Summary: This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (2) lacks a large portion of the 3' coding region and has a different 3' structure compared to variant 4. The encoded protein (isoform 2) has a distinct C-terminus and is shorter than isoform 4.