SNF5 (SMARCB1) (NM_001007468) Human Untagged Clone

CAT#: SC301215

SMARCB1 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 (SMARCB1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMARCB1
• Synonyms: BAF47; CSS3; hSNFS; INI1; MRD15; PPP1R144; RDT; RTPS1; Sfh1p; SNF5; SNF5L1; Snr1; SWNTS1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001007468 edited
  GAGCCCGGCTGAGGCGCCAGTACCCGGCCCGGTCCGCATTTCGCCTTCCGGCTTCGGTTT
  CCCTCGGCCCAGCACGCCCCGGCCCCGCCCCAGCCCTCCTGATCCCTCGCAGCCCGGCTC
  CGGCCGCCCGCCTCTGCCGCCGCAATGATGATGATGGCGCTGAGCAAGACCTTCGGGCAG
  AAGCCCGTGAAGTTCCAGCTGGAGGACGACGGCGAGTTCTACATGATCGGCTCCGAGGTG
  GGAAACTACCTCCGTATGTTCCGAGGTTCTCTGTACAAGAGATACCCCTCACTCTGGAGG
  CGACTAGCCACTGTGGAAGAGAGGAAGAAAATAGTTGCATCGTCACATGATCACGGATAC
  ACGACTCTAGCCACCAGTGTGACCCTGTTAAAAGCCTCGGAAGTGGAAGAGATTCTGGAT
  GGCAACGATGAGAAGTACAAGGCTGTGTCCATCAGCACAGAGCCCCCCACCTACCTCAGG
  GAACAGAAGGCCAAGAGGAACAGCCAGTGGGTACCCACCCTGCCCAACAGCTCCCACCAC
  TTAGATGCCGTGCCATGCTCCACAACCATCAACAGGAACCGCATGGGCCGAGACAAGAAG
  AGAACCTTCCCCCTTTGCTTTGATGACCATGACCCAGCTGTGATCCATGAGAACGCATCT
  CAGCCCGAGGTGCTGGTCCCCATCCGGCTGGACATGGAGATCGATGGGCAGAAGCTGCGA
  GACGCCTTCACCTGGAACATGAATGAGAAGTTGATGACGCCTGAGATGTTTTCAGAAATC
  CTCTGTGACGATCTGGATTTGAACCCGCTGACGTTTGTGCCAGCCATCGCCTCTGCCATC
  AGACAGCAGATCGAGTCCTACCCCACGGACAGCATCCTGGAGGACCAGTCAGACCAGCGC
  GTCATCATCAAGCTGAACATCCATGTGGGAAACATTTCCCTGGTGGACCAGTTTGAGTGG
  GACATGTCAGAGAAGGAGAACTCACCAGAGAAGTTTGCCCTGAAGCTGTGCTCGGAGCTG
  GGGTTGGGCGGGGAGTTTGTCACCACCATCGCATACAGCATCCGGGGACAGCTGAGCTGG
  CATCAGAAGACCTACGCCTTCAGCGAGAACCCTCTGCCCACAGTGGAGATTGCCATCCGG
  AACACGGGCGATGCGGACCAGTGGTGCCCACTGCTGGAGACTCTGACAGACGCTGAGATG
  GAGAAGAAGATCCGCGACCAGGACAGGAACACGAGGCGGATGAGGCGTCTTGCCAACACG
  GCCCCGGCCTGGTAACCAGCCCATCAGCACACGGCTCCCACGGAGCATCTCAGAAGATTG
  GGCCGCCTCTCCTCCATCTTCTGGCAAGGACAGAGGCGAGGGGACAGCCCAGCGCCATCC
  TGAGGATCGGGTGGGGGTGGAGTGGGGGCTTCCAGGTGGCCCTTCCCGGCACACATTCCA
  TTTGTTGAGCCCCAGTCCTGCCCCCCACCCCACCCTCCCTACCCCTCCCCAGTCTCTGGG
  GTCAGGAAGAAACCTTATTTTAGGTTGTGTTTTGTTTTTGTATAGGAGCCCCAGGCAGGG
  CTAGTAACAGTTTTTAAATAAAAGGCAACAGGTCATGTTCAATTTCTTCAACAAAAAAAA
  AAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001007468
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone is found to be a perfect match to NM_001007468.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007468.1, NP_001007469.1
• RefSeq Size: 1690 bp
• RefSeq ORF: 1131 bp
• Locus ID: 6598
• Cytogenetics: 22q11
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (2) uses an alternate in-frame donor splice site in the 5' coding region compared to variant 1. The resulting isoform (b) has the same N- and C-termini but lacks a 9 aa protein segment compared to isoform a.