FBXW7 (NM_001013415) Human Untagged Clone

CAT#: SC301782

FBXW7 (untagged)-Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXW7
• Synonyms: AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001013415, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCAAAACCGGGAAAACCTACTCTAAACCATGGCTTGGTTCCTGTTGATCTTAAAAGTGCAAAAGAGC
  CTCTACCACATCAAACTGTGATGAAGATATTTAGCATTAGCATCATTGCCCAAGGCCTCCCTTTTTGTCG
  AAGACGGATGAAAAGAAAGTTGGACCATGGTTCTGAGGTCCGCTCTTTTTCTTTGGGAAAGAAACCATGC
  AAAGTCTCAGAATATACAAGTACCACTGGGCTTGTACCATGTTCAGCAACACCAACAACTTTTGGGGACC
  TCAGAGCAGCCAATGGCCAAGGGCAACAACGACGCCGAATTACATCTGTCCAGCCACCTACAGGCCTCCA
  GGAATGGCTAAAAATGTTTCAGAGCTGGAGTGGACCAGAGAAATTGCTTGCTTTAGATGAACTCATTGAT
  AGTTGTGAACCAACACAAGTAAAACATATGATGCAAGTGATAGAACCCCAGTTTCAACGAGACTTCATTT
  CATTGCTCCCTAAAGAGTTGGCACTCTATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTACAAGCAGC
  TCAGACATGTCGCTACTGGAGAATTTTGGCTGAAGACAACCTTCTCTGGAGAGAGAAATGCAAAGAAGAG
  GGGATTGATGAACCATTGCACATCAAGAGAAGAAAAGTAATAAAACCAGGTTTCATACACAGTCCATGGA
  AAAGTGCATACATCAGACAGCACAGAATTGATACTAACTGGAGGCGAGGAGAACTCAAATCTCCTAAGGT
  GCTGAAAGGACATGATGATCATGTGATCACATGCTTACAGTTTTGTGGTAACCGAATAGTTAGTGGTTCT
  GATGACAACACTTTAAAAGTTTGGTCAGCAGTCACAGGCAAATGTCTGAGAACATTAGTGGGACATACAG
  GTGGAGTATGGTCATCACAAATGAGAGACAACATCATCATTAGTGGATCTACAGATCGGACACTCAAAGT
  GTGGAATGCAGAGACTGGAGAATGTATACACACCTTATATGGGCATACTTCCACTGTGCGTTGTATGCAT
  CTTCATGAAAAAAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGATATTGAGACAGGCC
  AGTGTTTACATGTTTTGATGGGTCATGTTGCAGCAGTCCGCTGTGTTCAATATGATGGCAGGAGGGTTGT
  TAGTGGAGCATATGATTTTATGGTAAAGGTGTGGGATCCAGAGACTGAAACCTGTCTACACACGTTGCAG
  GGGCATACTAATAGAGTCTATTCATTACAGTTTGATGGTATCCATGTGGTGAGTGGATCTCTTGATACAT
  CAATCCGTGTTTGGGATGTGGAGACAGGGAATTGCATTCACACGTTAACAGGGCACCAGTCGTTAACAAG
  TGGAATGGAACTCAAAGACAATATTCTTGTCTCTGGGAATGCAGATTCTACAGTTAAAATCTGGGATATC
  AAAACAGGACAGTGTTTACAAACATTGCAAGGTCCCAACAAGCATCAGAGTGCTGTGACCTGTTTACAGT
  TCAACAAGAACTTTGTAATTACCAGCTCAGATGATGGAACTGTAAAACTATGGGACTTGAAAACGGGTGA
  ATTTATTCGAAACCTAGTCACATTGGAGAGTGGGGGGAGTGGGGGAGTTGTGTGGCGGATCAGAGCCTCA
  AACACAAAGCTGGTGTGTGCAGTTGGGAGTCGGAATGGGACTGAAGAAACCAAGCTGCTGGTGCTGGACT
  TTGATGTGGACATGAAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013415
• ORF Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001013415.1, NP_001013433.1
• RefSeq Size: 3570
• RefSeq ORF: 1770
• Locus ID: 55294
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (3) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (3) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.