RTKN (NM_001015055) Human Untagged Clone

CAT#: SC301995

RTKN (untagged)-Human rhotekin (RTKN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RTKN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001015055, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCTCCCGAAACCACCGGAGCCGGGTCACCGTGGCCAGGGGCTCCGCCCTGGAGATGGAGTTCAAAC
  GCGGCCGCTTCCGACTCAGCCTCTTCAGCGACCTGCCCGAGGACACGGAGTTGCAGAGGAAGCTAGACCA
  TGAGATCCGGATGAGGGAAGGGGCCTGTAAGCTGCTGGCAGCCTGCTCCCAGCGAGAGCAGGCTCTGGAG
  GCCACCAAGAGCCTGCTAGTGTGCAACAGCCGCATCCTCAGCTACATGGGCGAGCTGCAGCGGCGCAAGG
  AGGCGCAGGTGCTGGGGAAGACAAGCCGGCGGCCTTCTGACAGTGGCCCGCCCGCTGAGCGCTCCCCCTG
  CCGCGGCCGGGTCTGCATCTCTGACCTCCGGATTCCACTCATGTGGAAGGACACAGAATATTTCAAGAAC
  AAAGGTGACTTGCACCGCTGGGCTGTGTTCCTGCTGCTGCAGCTGGGGGAACACATCCAGGACACAGAGA
  TGATCCTAGTGGACAGGACCCTCACAGACATCTCCTTTCAGAGCAATGTGCTCTTCGCTGAGGCGGGGCC
  AGACTTTGAACTGCGGTTAGAGCTGTATGGGGCCTGTGTGGAAGAAGAGGGGGCCCTGACTGGCGGCCCC
  AAGAGGCTTGCCACCAAACTCAGCAGCTCCCTGGGCCGCTCCTCAGGGAGGCGTGTCCGGGCATCGCTGG
  ACAGTGCTGGGGGTTCAGGGAGCAGTCCCATCTTGCTCCCCACCCCAGTTGTTGGTGGTCCTCGTTACCA
  CCTCTTGGCTCACACCACACTCACCCTGGCAGCAGTGCAAGATGGATTCCGCACACATGACCTCACCCTT
  GCCAGTCATGAGGAGAACCCTGCCTGGCTGCCCCTTTATGGTAGCGTGTGTTGCCGTCTGGCAGCTCAGC
  CTCTCTGCATGACTCAGCCCACTGCAAGTGGTACCCTCAGGGTGCAGCAAGCTGGGGAGATGCAGAACTG
  GGCACAAGTGCATGGAGTTCTGAAAGGCACAAACCTCTTCTGTTACCGGCAACCTGAGGATGCAGACACT
  GGGGAAGAGCCGCTGCTTACTATTGCTGTCAACAAGGAGACTCGAGTCCGGGCAGGGGAGCTGGACCAGG
  CTCTAGGACGGCCCTTCACCCTAAGCATCAGTAACCAGTATGGGGATGATGAGGTGACACACACCCTTCA
  GACAGAAAGTCGGGAAGCACTGCAGAGCTGGATGGAGGCTCTGTGGCAGCTTTTCTTTGACATGAGCCAA
  TGGAAGCAGTGCTGTGATGAAATCATGAAAATTGAAACTCCTGCTCCCCGGAAACCACCCCAAGCACTGG
  CAAAGCAGGGGTCCTTGTACCATGAGATGGCTATTGAGCCGCTGGATGACATCGCAGCGGTGACAGACAT
  CCTGACCCAGCGGGAGGGCGCAAGGCTGGAGACACCCCCACCCTGGCTGGCAATGTTTACAGACCAGCCT
  GCCCTGCCTAACCCCTGCTCGCCTGCCTCAGTGGCCCCAGCCCCAGACTGGACCCACCCCCTGCCCTGGG
  GGAGACCCCGAACCTTTTCCCTGGATGCTGTCCCCCCAGACCACTCCCCTAGGGCTCGCTCGGTTGCCCC
  CCTCCCACCTCAGCGATCCCCACGGACCAGAGGCCTCTGCAGCAAAGGCCAACCTCGCACTTGGCTCCAG
  TCACCAGTGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001015055
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001015055.1, NP_001015055.1
• RefSeq Size: 2208 bp
• RefSeq ORF: 1692 bp
• Locus ID: 6242
• Cytogenetics: 2p13.1
• Gene Summary: 'This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (a).