RNF98 (TRIM36) (NM_001017398) Human Untagged Clone

CAT#: SC302036

TRIM36 (untagged)-Human tripartite motif containing 36 (TRIM36), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRIM36
• Synonyms: ANPH; HAPRIN; RBCC728; RNF98
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001017398, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGGAGTCTGGGGAGATGAGTGAATTTGGCTACATCATGGAATTGATAGCTAAAGGCAAGGCTTCAG
  CAATGGGCCTGCAGCAAACCCATGAGCATTCCCGGTTGACTTCTAAAGGTGGAGAGGCCCGCTGTCCCTT
  TGAAATCTCTGAGGTTGGAAAGCAGTCTCTCCCAAGAAGAACTTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001017398
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017398.1, NP_001017398.1
• RefSeq Size: 906 bp
• RefSeq ORF: 186 bp
• Locus ID: 55521
• Cytogenetics: 5q22.3
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) lacks several 3' exons, but has a much shorter and alternate 3' segment, as compared to variant 1. The resulting isoform (3) has a much shorter and distinct C-terminus, as compared to isoform 1.