LGR6 (NM_001017404) Human Untagged Clone

CAT#: SC302039

LGR6 (untagged)-Human leucine-rich repeat containing G protein-coupled receptor 6 (LGR6), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LGR6
• Synonyms: GPCR; VTS20631
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001017404, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGCTTGGAGGGAGAGGGCCGCTCAGCGAGGGCGGGACAGAACCTCTCCCGGGCTGGGAGTGCACGGC
  GCGGTGCGCCCAGGGACCTCAGCATGAACAACCTCACAGAGCTTCAGCCTGGCCTCTTCCACCACCTGCG
  CTTCTTGGAGGAGCTGCGTCTCTCTGGGAACCATCTCTCACACATCCCAGGACAAGCATTCTCTGGTCTC
  TACAGCCTGAAAATCCTGATGCTGCAGAACAATCAGCTGGGAGGAATCCCCGCAGAGGCGCTGTGGGAGC
  TGCCGAGCCTGCAGTCGCTAGACCTGAATTATAACAAGCTGCAGGAGTTCCCTGTGGCCATCCGGACCCT
  GGGCAGACTGCAGGAACTGGGGTTCCATAACAACAACATCAAGGCCATCCCAGAAAAGGCCTTCATGGGG
  AACCCTCTGCTACAGACGATACACTTTTATGATAACCCAATCCAGTTTGTGGGAAGATCGGCATTCCAGT
  ACCTGCCTAAACTCCACACACTATCTCTGAATGGTGCCATGGACATCCAGGAGTTTCCAGATCTCAAAGG
  CACCACCAGCCTGGAGATCCTGACCCTGACCCGCGCAGGCATCCGGCTGCTCCCATCGGGGATGTGCCAA
  CAGCTGCCCAGGCTCCGAGTCCTGGAACTGTCTCACAATCAAATTGAGGAGCTGCCCAGCCTGCACAGGT
  GTCAGAAATTGGAGGAAATCGGCCTCCAACACAACCGCATCTGGGAAATTGGAGCTGACACCTTCAGCCA
  GCTGAGCTCCCTGCAAGCCCTGGATCTTAGCTGGAACGCCATCCGGTCCATCCACCCCGAGGCCTTCTCC
  ACCCTGCACTCCCTGGTCAAGCTGGACCTGACAGACAACCAGCTGACCACACTGCCCCTGGCTGGACTTG
  GGGGCTTGATGCATCTGAAGCTCAAAGGGAACCTTGCTCTCTCCCAGGCCTTCTCCAAGGACAGTTTCCC
  AAAACTGAGGATCCTGGAGGTGCCTTATGCCTACCAGTGCTGTCCCTATGGGATGTGTGCCAGCTTCTTC
  AAGGCCTCTGGGCAGTGGGAGGCTGAAGACCTTCACCTTGATGATGAGGAGTCTTCAAAAAGGCCCCTGG
  GCCTCCTTGCCAGACAAGCAGAGAACCACTATGACCAGGACCTGGATGAGCTCCAGCTGGAGATGGAGGA
  CTCAAAGCCACACCCCAGTGTCCAGTGTAGCCCTACTCCAGGCCCCTTCAAGCCCTGTGAGTACCTCTTT
  GAAAGCTGGGGCATCCGCCTGGCCGTGTGGGCCATCGTGTTGCTCTCCGTGCTCTGCAATGGACTGGTGC
  TGCTGACCGTGTTCGCTGGCGGGCCTGTCCCCCTGCCCCCGGTCAAGTTTGTGGTAGGTGCGATTGCAGG
  CGCCAACACCTTGACTGGCATTTCCTGTGGCCTTCTAGCCTCAGTCGATGCCCTGACCTTTGGTCAGTTC
  TCTGAGTACGGAGCCCGCTGGGAGACGGGGCTAGGCTGCCGGGCCACTGGCTTCCTGGCAGTACTTGGGT
  CGGAGGCATCGGTGCTGCTGCTCACTCTGGCCGCAGTGCAGTGCAGCGTCTCCGTCTCCTGTGTCCGGGC
  CTATGGGAAGTCCCCCTCCCTGGGCAGCGTTCGAGCAGGGGTCCTAGGCTGCCTGGCACTGGCAGGGCTG
  GCCGCCGCGCTGCCCCTGGCCTCAGTGGGAGAATACGGGGCCTCCCCACTCTGCCTGCCCTACGCGCCAC
  CTGAGGGTCAGCCAGCAGCCCTGGGCTTCACCGTGGCCCTGGTGATGATGAACTCCTTCTGTTTCCTGGT
  CGTGGCCGGTGCCTACATCAAACTGTACTGTGACCTGCCGCGGGGCGACTTTGAGGCCGTGTGGGACTGC
  GCCATGGTGAGGCACGTGGCCTGGCTCATCTTCGCAGACGGGCTCCTCTACTGTCCCGTGGCCTTCCTCA
  GCTTTGCCTCCATGCTGGGCCTCTTCCCTGTCACGCCCGAGGCCGTCAAGTCTGTCCTGCTGGTGGTGCT
  GCCCCTGCCTGCCTGCCTCAACCCACTGCTGTACCTGCTCTTCAACCCCCACTTCCGGGATGACCTTCGG
  CGGCTTCGGCCCCGCGCAGGGGACTCAGGGCCCCTAGCCTATGCTGCGGCCGGGGAGCTGGAGAAGAGCT
  CCTGTGATTCTACCCAGGCCCTGGTAGCCTTCTCTGATGTGGATCTCATTCTGGAAGCTTCTGAAGCTGG
  GCGGCCCCCTGGGCTGGAGACCTATGGCTTCCCCTCAGTGACCCTCATCTCCTGTCAGCAGCCAGGGGCC
  CCCAGGCTGGAGGGCAGCCATTGTGTAGAGCCAGAGGGGAACCACTTTGGGAACCCCCAACCCTCCATGG
  ATGGAGAACTGCTGCTGAGGGCAGAGGGATCTACGCCAGCAGGTGGAGGCTTGTCAGGGGGTGGCGGCTT
  TCAGCCCTCTGGCTTGGCCTTTGCTTCACACGTGTAA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_001017404
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017404.1, NP_001017404.1
• RefSeq Size: 3041 bp
• RefSeq ORF: 2487 bp
• Locus ID: 59352
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. The encoded protein is a glycoprotein hormone receptor with a large N-terminal extracellular domain that contains leucine-rich repeats important for the formation of a horseshoe-shaped interaction motif for ligand binding. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) contains an alternate in-frame exon in the 5' coding region and uses a downstream start codon, compared to variant 1. The encoded protein (isoform 3) has a shorter N-terminus, compared to isoform 1.