KCNK2 (NM_001017424) Human Untagged Clone

CAT#: SC302049

KCNK2 (untagged)-Human potassium channel, subfamily K, member 2 (KCNK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNK2
• Synonyms: hTREK-1c; hTREK-1e; K2p2.1; TPKC1; TREK; TREK-1; TREK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001017424 edited
  ATGATGAACCCACGAGCAAAAAGGGATTTCTACTTGGCGGCACCTGACTTGCTGGATCCT
  AAATCTGCCGCTCAGAACTCCAAACCGAGGCTCTCGTTTTCCACGAAACCCACAGTGCTT
  GCTTCCCGGGTGGAGAGTGACACGACCATTAATGTTATGAAATGGAAGACGGTCTCCACG
  ATATTCCTGGTGGTTGTCCTCTATCTGATCATCGGAGCCACCGTGTTCAAAGCATTGGAG
  CAGCCTCATGAGATTTCACAGAGGACCACCATTGTGATCCAGAAGCAAACATTCATATCC
  CAACATTCCTGTGTCAATTCGACGGAGCTGGATGAACTCATTCAGCAAATAGTGGCAGCA
  ATAAATGCAGGGATTATACCGTTAGGAAACACCTCCAATCAAATCAGTCACTGGGATTTG
  GGAAGTTCCTTCTTCTTTGCTGGCACTGTTATTACAACCATAGGATTTGGAAACATCTCA
  CCACGCACAGAAGGCGGCAAAATATTCTGTATCATCTATGCCTTACTGGGAATTCCCCTC
  TTTGGTTTTCTCTTGGCTGGAGTTGGAGATCAGCTAGGCACCATATTTGGAAAAGGAATT
  GCCAAAGTGGAAGATACGTTTATTAAGTGGAATGTTAGTCAGACCAAGATTCGCATCATC
  TCAACAATCATATTTATACTATTTGGCTGTGTACTCTTTGTGGCTCTGCCTGCGATCATA
  TTCAAACACATAGAAGGCTGGAGTGCCCTGGACGCCATTTATTTTGTGGTTATCACTCTA
  ACAACTATTGGATTTGGTGACTACGTTGCAGGTGGATCCGATATTGAATATCTGGACTTC
  TATAAGCCTGTCGTGTGGTTCTGGATCCTTGTAGGGCTTGCTTACTTTGCTGCTGTCCTG
  AGCATGATTGGAGATTGGCTCCGAGTGATATCTAAAAAGACAAAAGAAGAGGTGGGAGAG
  TTCAGAGCACACGCTGCTGAGTGGACAGCCAACGTCACAGCCGAATTCAAAGAAACCAGG
  AGGCGACTGAGTGTGGAGATTTATGACAAGTTCCAGCGGGCCACCTCCATCAAGCGGAAG
  CTCTCGGCAGAACTGGCTGGAAACCACAATCAGGAGCTGACTCCTTGTAGGAGGACCCTG
  TCAGTGAACCACCTGACCAGCGAGAGGGATGTCTTGCCTCCCTTACTGAAGACTGAGAGT
  ATCTATCTGAATGGTTTGACGCCACACTGTGCTGGTGAAGAGATTGCTGTGATTGAGAAC
  ATCAAATAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001017424 unedited
  TCACATATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGATGATGAACC
  CACGAGCAAAAAGGATTTCTACTTGGCGGCACCTGACTTGCTGGATCCTAAATCTGCCGC
  TCAGAACTCCAAACCGAGGCTCTCGTTTTCCACGAAACCCACAGTGCTTGCTTCCCGGGT
  GGAGAGTGACACGACCATTAATGTTATGAAATGGAAGACGGTCTCCACGATATTCCTGGT
  GGTTGTCCTCTATCTGATCATCGGAGCCACCGTGTTCAAAGCATTGGAGCAGCCTCATGA
  GATTTCACAGAGGACCACCATTGTGATCCAGAAGCAAACATTCATATCCCAACATTCCTG
  TGTCAATTCGACGGAGCTGGATGAACTCATTCAGCAAATAGTGGCAGCAATAAATGCAGG
  GATTATACCGTTAGGAAACACCTCCAATCAAATCAGTCACTGGGATTTGGGAAGTTCCTT
  CTTCTTTGCTGGCACTGTTATTACAACCATAGGATTTGGAAACATCTCACCACGCACAGA
  AGGCGGCAAAATATTCTGTATCATCTATGCCTTACTGGGAATTCCCCTCTTTGGTTTTCT
  CTTGGCTGGAGTTGGAGATCAGCTAGGCACCATATTTGGAAAAGGAATTGCCAAAGTGGA
  AGATACGTTTATTAAGTGGAATGTTAGTCAGACCAAGATTCGCATCATCTCAACAATCAT
  ATTTATACTATTTGGNCTGTGTACCTCTTTGTGGCTCTGCCTGCGATCATATTCAAACCA
  TAGAAAGCTGNAGTGCCCTGGACGCCATTATTTTTGTGGTATCACTCTAACACCTATTGG
  ATTTGGTGACTACGTTGCAGGTGGATCCGATATTGAATATCTGGGACTTCTTAAACCTGT
  CCTGGGGGTCCTGGACCCT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001017424 unedited
  GTAGACGCCTCCCAGTGAGATGGATCAAAGGTGTTGGGTTGGAATCGCTAGGCACCATAT
  TTGGAAAAGGAATTGCCAAAGTGGAAGATACGTTTATTAAGTGGAATGTTAGTCAGACCA
  AGATTCGCATCATCTCAACAATCATATTTATACTATTTGGCTGTGTACTCTTTGTGGCTC
  TGCCTGCGATCATATTCAAACACATAGAAGGCTGGAGTGCCCTGGACGCCATTTATTTTG
  TGGTTATCACTCTAACAACTATTGGATTTGGTGACTACGTTGCAGGTGGATCCGATATTG
  AATATCTGGACTTCTATAAGCCTGTCGTGTGGTTCTGGATCCTTGTAGGGCTTGCTTACT
  TTGCTGCTGTCCTGAGCATGATTGGAGATTGGCTCCGAGTGATATCTAAAAAGACAAAAG
  AAGAGGTGGGAGAGTTCAGAGCACACGCTGCTGAGTGGACAGCCAACGTCACAGCCGAAT
  TCAAAGAAACCAGGAGGCGACTGAGTGTGGAGATTTATGACAAGTTCCAGCGGGCCACCT
  CCATCAAGCGGAAGCTCTCGGCAGAACTGGCTGGAAACCACAATCAGGAGCTGACTCCTT
  GTAGGAGGACCCTGTCAGTGAACCACCTGACCAGCGAGAGGGATGTCTTGCCTCCCTTAC
  TGAAGACTGAGAGTATCTATCTGAATGGTTTGACGCCACACTGTGCTGGTGAAGAGATTG
  CTGTGATTGAGAACATCAAATAGCCCTCTCTTTAAATAACCTTAGGCATAGCCATAGGTG
  AGGACTTCTCTATGCTCTTTATGACTGTTGCTGGTAGCATTTTTTAAATTGTGCATGAGC
  TCAAAGGGGGAACAAAATAGATACACCCATCATGGTCATCTATCATCAGAGATTTGGAAT
  TCTGAGCCAGCCCTTTCTTTCTGATGATGCTTGTTGGAACCGGTTCCACTTCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_001017424
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017424.1, NP_001017424.1
• RefSeq Size: 3340 bp
• RefSeq ORF: 1269 bp
• Locus ID: 3776
• Cytogenetics: 1q41
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'This gene encodes one of the members of the two-pore-domain background potassium channel protein family. This type of potassium channel is formed by two homodimers that create a channel that leaks potassium out of the cell to control resting membrane potential. The channel can be opened, however, by certain anesthetics, membrane stretching, intracellular acidosis, and heat. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (a).