HSP90AA1 (NM_001017963) Human Untagged Clone

CAT#: SC302090

HSP90AA1 (untagged)-Human heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSP90AA1
• Synonyms: EL52; HEL-S-65p; HSP86; Hsp89; HSP89A; Hsp90; HSP90A; HSP90N; Hsp103; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001017963 edited
  ATGCCCCCGTGTTCGGGCGGGGACGGCTCCACCCCTCCTGGGCCCTCCCTTCGGGACAGG
  GACTGTCCCGCCCAGAGTGCTGAATACCCGCGCGACCGTCTGGATCCCCGCCCAGGAAGC
  CCCTCTGAAGCCTCCTCGCCGCCGTTTCTGAGAAGGGCACCTGTTAACTGGTACCAAGAA
  AAGGCCCAAGTGTTTCTCTGGCATCTGTTGGTGTCTGGATCCACCACTCTACTCTGTCTC
  TGGAAACAGCCCTTCCACGTCTCTGCATTCCCTGTCACTGCGTCACTGGCCTTCAGACAG
  AGCCAAGGTGCAGGGCAACACCTCTACAAGGATCTGCAGCCATTTATATTGCTTAGGCTA
  CTGATGCCTGAGGAAACCCAGACCCAAGACCAACCGATGGAGGAGGAGGAGGTTGAGACG
  TTCGCCTTTCAGGCAGAAATTGCCCAGTTGATGTCATTGATCATCAATACTTTCTACTCG
  AACAAAGAGATCTTTCTGAGAGAGCTCATTTCAAATTCATCAGATGCATTGGACAAAATC
  CGGTATGAAAGCTTGACAGATCCCAGTAAATTAGACTCTGGGAAAGAGCTGCATATTAAC
  CTTATACCGAACAAACAAGATCGAACTCTCACTATTGTGGATACTGGAATTGGAATGACC
  AAGGCTGACTTGATCAATAACCTTGGTACTATCGCCAAGTCTGGGACCAAAGCGTTCATG
  GAAGCTTTGCAGGCTGGTGCAGATATCTCTATGATTGGCCAGTTCGGTGTTGGTTTTTAT
  TCTGCTTATTTGGTTGCTGAGAAAGTAACTGTGATCACCAAACATAACGATGATGAGCAG
  TACGCTTGGGAGTCCTCAGCAGGGGGATCATTCACAGTGAGGACAGACACAGGTGAACCT
  ATGGGTCGTGGAACAAAAGTTATCCTACACCTGAAAGAAGACCAAACTGAGTACTTGGAG
  GAACGAAGAATAAAGGAGATTGTGAAGAAACATTCTCAGTTTATTGGATATCCCATTACT
  CTTTTTGTGGAGAAGGAACGTGATAAAGAAGTAAGCGATGATGAGGCTGAAGAAAAGGAA
  GACAAAGAAGAAGAAAAAGAAAAAGAAGAGAAAGAGTCGGAAGACAAACCTGAAATTGAA
  GATGTTGGTTCTGATGAGGAAGAAGAAAAGAAGGATGGTGACAAGAAGAAGAAGAAGAAG
  ATTAAGGAAAAGTACATCGATCAAGAAGAGCTCAACAAAACAAAGCCCATCTGGACCAGA
  AATCCCGACGATATTACTAATGAGGAGTACGGAGAATTCTATAAGAGCTTGACCAATGAC
  TGGGAAGATCACTTGGCAGTGAAGCATTTTTCAGTTGAAGGACAGTTGGAATTCAGAGCC
  CTTCTATTTGTCCCACGACGTGCTCCTTTTGATCTGTTTGAAAACAGAAAGAAAAAGAAC
  AATATCAAATTGTATGTACGCAGAGTTTTCATCATGGATAACTGTGAGGAGCTAATCCCT
  GAATATCTGAACTTCATTAGAGGGGTGGTAGACTCGGAGGATCTCCCTCTAAACATATCC
  CGTGAGATGTTGCAACAAAGCAAAATTTTGAAAGTTATCAGGAAGAATTTGGTCAAAAAA
  TGCTTAGAACTCTTTACTGAACTGGCGGAAGATAAAGAGAACTACAAGAAATTCTATGAG
  CAGTTCTCTAAAAACATAAAGCTTGGAATACACGAAGACTCTCAAAATCGGAAGAAGCTT
  TCAGAGCTGTTAAGGTACTACACATCTGCCTCTGGTGATGAGATGGTTTCTCTCAAGGAC
  TACTGCACCAGAATGAAGGAGAACCAGAAACATATCTATTATATCACAGGTGAGACCAAG
  GACCAGGTAGCTAACTCAGCCTTTGTGGAACGTCTTCGGAAACATGGCTTAGAAGTGATC
  TATATGATTGAGCCCATTGATGAGTACTGTGTCCAACAGCTGAAGGAATTTGAGGGGAAG
  ACTTTAGTGTCAGTCACCAAAGAAGGCCTGGAACTTCCAGAGGATGAAGAAGAGAAAAAG
  AAGCAGGAAGAGAAAAAAACAAAGTTTGAGAACCTCTGCAAAATCATGAAAGACATATTG
  GAGAAAAAAGTTGAAAAGGTGGTTGTGTCAAACCGATTGGTGACATCTCCATGCTGTATT
  GTCACAAGCACATATGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCTA
  AGAGACAACTCAACAATGGGTTACATGGCAGCAAAGAAACACCTGGAGATAAACCCTGAC
  CATTCCATTATTGAGACCTTAAGGCAAAAGGCAGAGGCTGATAAGAACGACAAGTCTGTG
  AAGGATCTGGTCATCTTGCTTTATGAAACTGCGCTCCTGTCTTCTGGCTTCAGTCTGGAA
  GATCCCCAGACACATGCTAACAGGATCTACAGGATGATCAAACTTGGTCTGGGTATTGAT
  GAAGATGACCCTACTGCTGATGATACCAGTGCTGCTGTAACTGAAGAAATGCCACCCCTT
  GAAGGAGATGACGACACATCACGCATGGAAGAAGTAGACTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001017963 unedited
  CGCTCCTTTAGGCGGCCGCGAATTCGGCACGAGGCTTCCCTGGCGTGCTCCACCCGACTG
  GGCGTCCGCAGGCTCCTCCCCCGGGTGTGGCCTCCGGGCGGCATGGCTGCTTCCCAAGGT
  GATGCCGGCTTCAGCTAGTGGGGTCTAGTTGACCGTTCCGCAGCCGCCAGGGCCAGCGGA
  AAGCCGGTCAGGGGGAACCGCGGCGGGGCTGGTGTCATGAGCCTGAGGTGAACTTGAGGG
  TGCCTCCTCAGCGGTCTCCCGCCCTGCCCTGAGGGGCGCCGGGACCCCAAAGAGCGGAGG
  AAGAAGCGCCACCCCGACGGCCACCGCTTCGGAGCCAGCACGCGGGGTACCCTACGGGGA
  GCGCGGATGCCCCCGTGTTCGGGCGGGNGACGGCTCCACCCCTCCTGGGCCCTCCCTTCG
  GGACAGGGGACTGTCCCGCCCAGAGTGCTGAATACCCGCGCGAACCGTCTGGATCCCCGC
  CCAGGAAGCCCCTCTGAAGCCTCCTGNNCGNNCGTTTCTGAGAAAGGCACCTGTTAACTG
  GTACCAAGAAAAGCCCAAGTGTTTCTCTGCATCTGGTTGGTGTNCTGAATCACCACTCTA
  CTCTGTCTCTGAAAACAGCCTTCCACGTCTCTGCATTCCCTGTCACTGCGTCACTGGCCT
  TCAACAAGCCAGGGGGCAGGGAACACCTTACAGGATCTGAGCCTTAATATGCTTGGCTAC
  TGAGCCTGAGGAACCAGACCAGACAACATGAGGGGGGGGGGGGTAAAAATTTCCTTTTAG
  AAAAATTGCAAGGGAGCATTAAATAAAACTTTTTATGACAAAAAATTTTTTGAAGAGTAT
  TTAAATATAAAAGCTGAAAAACCGTTGAAATTGCAAACCCTAATTACTTGGAAAATTGTT
  AAACTTTACAAAAAAATGACTTTTTTGGGTAA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001017963 unedited
  ATCATGNAAAGCTCAAGCCCTAAAAGACAACTCAACAATGGGTTACATGGCAGCAAAGAA
  CACCTGGAGATAAACCCTGACCATTCCATTATTGAGACCTTAAGGCAAAAGGCAGAGGCT
  GATAAGAACGACAAGTCTGTGAAGGATCTGGTCATCTTGCTTTATGAAACTGCGCTCCTG
  TCTTCTGGCTTCAGTCTGGAAGATCCCCAGACACATGCTAACAGGATCTACAGGATGATC
  AAACTTGGTCTGGGTATTGATGAAGATGACCCTACTGCTGATGATACCAGTGCTGCTGTA
  ACTGAAGAAATGCCACCCCTTGAAGGAGATGACGACACATCACGCATGGAAGAAGTAGAC
  TAATCTCTGGCTGAGGGATGACTTACCTGTTCAGTACTCTACAATTCCTCTGATAATATA
  TTTTCAAGGATGTTTTTCTTTATTTTTGTTAATATTAAAAAGTCTGTATGGCATGACAAC
  TACTTTAAGGNGAAGATAAGATTTCTGTCTACTAAGTGATGCTGTGATACCTTANGCACT
  AAAGCAGAGCTAGTAATGCTTTTTGAGTTTCATGTTGGTTTATTTTCACAGATTGGGGTA
  ACGTGCACTGNTAGACGTATGTAACATGATGTTAACTTTGTGTGGTCTANAGTGTTTAGC
  TGTCAAGCCGGATGCCTAANTAGACCAAATCTTGTATTTGAATGTTCTGAGCTGTATCTT
  GATGTTTAGAAAAGTATTCGTTACATCTTGTAAGATCTACCTTTTTGACCTTTCATTCCC
  TGTATTTGACAATTCGCCTGTTCTAATCCTTAGAAAAAGGTTAAACTGACCACTTTGTGG
  AGGTTCCCTCCCAGGGCTTGTTTTCCAAGAAAATATTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001017963
• Insert Size: 3600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There is 5 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 2 aa and deletion of 1 aa.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017963.1, NP_001017963.1
• RefSeq Size: 3512 bp
• RefSeq ORF: 2565 bp
• Locus ID: 3320
• Cytogenetics: 14q32.31
• Protein Families: Druggable Genome
• Protein Pathways: Antigen processing and presentation, NOD-like receptor signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer
• Gene Summary: 'The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).