PODXL (NM_001018111) Human Untagged Clone

CAT#: SC302189

PODXL (untagged)-Human podocalyxin-like (PODXL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PODXL
• Synonyms: Gp200; PC; PCLP; PCLP-1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001018111 edited
  ATGCGCTGCGCGCTGGCGCTCTCGGCGCTGCTGCTACTGTTGTCAACGCCGCCGCTGCTG
  CCGTCGTCGCCGTCGCCGTCGCCGTCGCCCTCCCAGAATGCAACCCAGACTACTACGGAC
  TCATCTAACAAAACAGCACCGACTCCAGCATCCAGTGTCACCATCATGGCTACAGATACA
  GCCCAGCAGAGCACAGTCCCCACTTCCAAGGCCAACGAAATCTTGGCCTCAGTCAAGGCG
  ACCACCCTTGGTGTATCCAGTGACTCACCGGGGACTACAACCCTGGCTCAGCAAGTCTCA
  GGCCCAGTCAACACTACCGTGGCTAGAGGAGGCGGCTCAGGCAACCCTACTACCACCATC
  GAGAGCCCCAAGAGCACAAAAAGTGCAGACACCACTACAGTTGCAACCTCCACAGCCACA
  GCTAAACCTAACACCACAAGCAGCCAGAATGGAGCAGAAGATACAACAAACTCTGGGGGG
  AAAAGCAGCCACAGTGTGACCACAGACCTCACATCCACTAAGGCAGAACATCTGACGACC
  CCTCACCCTACAAGTCCACTTAGCCCCCGACAACCCACTTCGACGCATCCTGTGGCCACC
  CCAACAAGCTCGGGACATGACCATCTTATGAAAATTTCAAGCAGTTCAAGCACTGTGGCT
  ATCCCTGGCTACACCTTCACAAGCCCGGGGATGACCACCACCCTACTAGAGACAGTGTTT
  CACCATGTCAGCCAGGCTGGTCTTGAACTCCTGACCTCGGGTGATCTGCCCACCTTGGCC
  TCCCAAAGTGCTGGGATTACAGCGTCATCGGTTATCTCGCAAAGAACTCAACAGACCTCC
  AGTCAGATGCCAGCCAGCTCTACGGCCCCTTCCTCCCAGGAGACAGTGCAGCCCACGAGC
  CCGGCAACGGCATTGAGAACACCTACCCTGCCAGAGACCATGAGCTCCAGCCCCACAGCA
  GCATCAACTACCCACCGATACCCCAAAACACCTTCTCCCACTGTGGCTCATGAGAGTAAC
  TGGGCAAAGTGTGAGGATCTTGAGACACAGACACAGAGTGAGAAGCAGCTCGTCCTGAAC
  CTCACAGGAAACACCCTCTGTGCAGGGGGCGCTTCGGATGAGAAATTGATCTCACTGATA
  TGCCGAGCAGTCAAAGCCACCTTCAACCCGGCCCAAGATAAGTGCGGCATACGGCTGGCA
  TCTGTTCCAGGAAGTCAGACCGTGGTCGTCAAAGAAATCACTATTCACACTAAGCTCCCT
  GCCAAGGATGTGTACGAGCGGCTGAAGGACAAATGGGATGAACTAAAGGAGGCAGGGGTC
  AGTGACATGAAGCTAGGGGACCAGGGGCCACCGGAGGAGGCCGAGGACCGCTTCAGCATG
  CCCCTCATCATCACCATCGTCTGCATGGCATCATTCCTGCTCCTCGTGGCGGCCCTCTAT
  GGCTGCTGCCACCAGCGCCTCTCCCAGAGGAAGGACCAGCAGCGGCTAACAGAGGAGCTG
  CAGACAGTGGAGAATGGTTACCATGACAACCCAACACTGGAAGTGATGGAGACCTCTTCT
  GAGATGCAGGAGAAGAAGGTGGTCAGCCTCAACGGGGAGCTGGGGGACAGCTGGATCGTC
  CCTCTGGACAACCTGACCAAGGACGACCTGGATGAGGAGGAAGACACACACCTCTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001018111
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001018111.1, NP_001018121.1
• RefSeq Size: 5999 bp
• RefSeq ORF: 1677 bp
• Locus ID: 5420
• Cytogenetics: 7q32.3
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).