beta 1 Spectrin (SPTB) (NM_001024858) Human Untagged Clone

CAT#: SC302308

SPTB (untagged)-Human spectrin, beta, erythrocytic (SPTB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPTB
• Synonyms: EL3; HS2; HSPTB1; SPH2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001024858, the custom clone sequence may differ by one or more nucleotides
  ATGACATCGGCCACAGAGTTTGAAAATGTGGGCAACCAGCCACCTTACAGCAGGATCAAT
  GCCCGCTGGGACGCCCCAGACGACGAGCTGGATAATGACAACAGCTCAGCCAGGCTCTTT
  GAGAGGTCCCGGATAAAGGCCTTGGCAGATGAGCGGGAAGTTGTTCAGAAAAAGACCTTC
  ACGAAATGGGTGAACTCGCACCTGGCTCGAGTGTCCTGCCGCATCACCGATCTCTACAAG
  GACCTGCGGGATGGGCGCATGCTCATCAAGCTGCTGGAGGTGCTCTCTGGAGAGATGCTG
  CCAAAGCCCACCAAGGGGAAGATGCGCATCCACTGCCTGGAGAATGTGGACAAGGCTCTC
  CAGTTCCTCAAGGAGCAGCGTGTACACCTGGAGAACATGGGCTCCCACGACATTGTAGAT
  GGCAACCACCGCCTGGTCCTGGGCCTCATCTGGACCATCATCCTCCGCTTCCAGATTCAG
  GACATTGTGGTCCAAACTCAGGAAGGTCGTGAAACACGCTCAGCCAAGGATGCGTTGCTG
  TTGTGGTGTCAGATGAAGACGGCAGGCTACCCTCATGTTAATGTCACCAACTTTACCTCC
  AGCTGGAAGGATGGCTTGGCCTTTAATGCCCTGATACACAAGCACCGGCCCGACCTGATC
  GACTTTGATAAGCTGAAGGACTCCAATGCCCGGCACAACCTGGAGCACGCATTCAATGTG
  GCTGAGCGCCAGCTGGGCATCATCCCGCTCCTCGACCCCGAAGATGTCTTTACGGAAAAC
  CCTGATGAGAAATCCATCATCACCTATGTGGTGGCCTTTTACCACTACTTCTCCAAGATG
  AAGGTGCTGGCAGTGGAGGGCAAGCGTGTCGGCAAGGTTATTGACCATGCCATTGAGACT
  GAGAAGATGATTGAAAAGTACAGCGGGCTAGCCTCGGACCTGCTCACCTGGATCGAGCAG
  ACCATCACTGTCCTGAACAGCCGCAAGTTTGCCAACTCGCTGACGGGCGTCCAGCAGCAG
  CTGCAGGCCTTCAGCACCTACCGCACCGTGGAGAAGCCGCCCAAGTTTCAAGAGAAGGGG
  AATCTGGAAGTTCTACTTTTTACCATCCAGTCCCGGATGAGAGCCAACAATCAGAAAGTG
  TACACACCCCACGATGGGAAACTAGTGTCTGACATCAACAGGGCCTGGGAAAGCCTGGAG
  GAAGCTGAGTATCGGCGGGAGCTGGCCCTGAGAAATGAGCTCATTCGGCAGGAGAAGCTA
  GAGCAACTGGCCCGGCGCTTTGACCGGAAGGCCGCAATGAGAGAGACCTGGCTCAGTGAA
  AACCAGCGCCTCGTGGCCCAGGATAACTTTGGGTATGACCTGGCAGCTGTGGAGGCCGCC
  AAGAAGAAGCATGAGGCCATCGAGACCGACACGGCTGCCTACGAGGAGCGGGTGAGAGCC
  CTGGAGGACCTGGCTCAGGAGCTGGAGAAAGAGAACTACCATGACCAGAAGCGCATCACG
  GCCCGCAAGGACAATATACTGCGCCTATGGAGCTACCTGCAGGAGCTGCTGCAGTCCCGG
  CGCCAGAGGCTCGAGACCACCCTGGCACTGCAGAAGCTCTTCCAGGACATGCTGCACAGC
  ATCGACTGGATGGATGAGATCAAGGCTCACCTCTTGTCTGCCGAGTTTGGGAAGCACTTG
  TTGGAGGTTGAAGACCTGCTACAGAAGCACAAGTTGATGGAAGCTGACATCGCCATCCAA
  GGGGACAAAGTGAAGGCCATCACCGCAGCCACCCTGAAGTTCACCGAGGGGAAAGGGTAC
  CAGCCTTGTGACCCCCAGGTCATCCAGGACCGCATCAGCCACTTGGAGCAGTGCTTTGAG
  GAGCTGAGCAACATGGCAGCTGGGCGGAAGGCCCAACTGGAGCAGTCCAAACGACTCTGG
  AAGTTCTTCTGGGAGATGGATGAGGCTGAGAGCTGGATCAAGGAGAAGGAGCAGATCTAT
  TCTTCCCTGGACTATGGCAAAGACCTGACCAGTGTGCTCATCTTACAGCGCAAGCACAAG
  GCCTTTGAGGATGAGCTCCGTGGGCTGGATGCTCACCTGGAGCAGATCTTCCAGGAGGCT
  CATGGCATGGTTGCGCGCAAGCAGTTTGGGCACCCGCAGATCGAGGCCCGCATAAAGGAG
  GTGTCGGCACAGTGGGACCAGCTGAAGGACCTGGCTGCCTTCTGCAAGAAGAACCTCCAG
  GATGCTGAGAACTTTTTCCAGTTCCAGGGCGATGCGGATGACCTGAAGGCTTGGCTGCAA
  GACGCCCACCGGCTGCTCTCTGGTGAAGATGTGGGGCAGGACGAAGGGGCCACGCGGGCC
  CTGGGGAAAAAGCACAAGGACTTCCTGGAGGAGCTGGAGGAGAGCCGTGGGGTGATGGAG
  CACCTGGAGCAGCAGGCCCAGGGATTCCCCGAAGAGTTTCGGGATTCCCCAGATGTGACC
  CATCGGCTGCAGGCCCTGCGGGAGCTCTACCAACAGGTGGTGGCCCAGGCGGACCTGCGT
  CAGCAGAGGCTGCAGGAAGCCCTGGACCTGTACACGGTGTTCGGGGAGACAGACGCCTGT
  GAGCTGTGGATGGGAGAGAAGGAGAAGTGGCTGGCCGAGATGGAAATGCCAGACACCCTG
  GAGGACCTGGAGGTCGTGCAGCACAGGTTCGACATCCTGGACCAGGAGATGAAGACCTTG
  ATGACTCAGATTGATGGTGTGAACCTCGCTGCCAACAGCTTGGTAGAGAGTGGCCACCCA
  CGCAGCAGGGAGGTGAAGCAGTACCAGGACCATCTGAACACCAGGTGGCAGGCATTTCAG
  ACCCTGGTGTCGGAGCGGCGGGAGGCTGTGGACTCAGCCCTCCGAGTGCACAACTACTGC
  GTAGATTGCGAGGAGACCAGCAAGTGGATCACGGACAAGACAAAGGTAGTGGAGTCCACA
  AAAGACCTGGGGCGGGACCTGGCAGGTATCATCGCCATCCAGAGGAAGTTGTCAGGGCTG
  GAGCGTGACGTGGCCGCCATCCAGGCCCGTGTGGATGCCCTGGAGCGTGAGTCCCAGCAG
  CTGATGGACTCGCACCCTGAGCAGAAGGAGGATATTGGTCAGCGGCAAAAACACTTGGAG
  GAGCTGTGGCAGGGCCTGCAGCAATCCCTGCAGGGCCAGGAGGACTTGCTGGGGGAAGTC
  AGCCAGCTGCAGGCCTTCCTGCAGGATCTGGATGACTTCCAGGCCTGGCTCTCCATCACC
  CAGAAGGCTGTGGCCTCTGAGGACATGCCCGAATCCCTCCCAGAGGCTGAGCAGCTCCTG
  CAGCAGCATGCAGGTATCAAGGATGAGATTGACGGGCACCAAGACAGCTACCAGCGTGTT
  AAGGAGTCTGGGGAGAAAGTGATCCAAGGCCAGACGGACCCAGAGTATCTGCTTCTGGGC
  CAGCGGCTGGAGGGCCTGGATACTGGCTGGAATGCCCTGGGCAGGATGTGGGAGAGCCGC
  AGCCACACCCTCGCTCAGTGCCTTGGCTTCCAGGAGTTCCAGAAAGATGCCAAGCAGGCT
  GAAGCCATCCTCAGCAACCAGGAATACACTCTGGCTCACTTGGAGCCCCCAGACTCCCTG
  GAAGCTGCAGAGGCTGGGATCCGGAAGTTTGAGGATTTCTTGGGGTCTATGGAGAACAAC
  CGGGATAAGGTCTTGAGTCCTGTGGACTCTGGAAACAAGCTGGTAGCTGAGGGAAACCTA
  TACTCAGACAAGATCAAGGAGAAGGTGCAGCTGATTGAGGACAGGCACAGGAAGAACAAC
  GAGAAGGCCCAGGAGGCCTCTGTCCTACTGAGAGACAACCTGGAGCTACAGAACTTCCTC
  CAGAACTGCCAGGAGCTCACTCTCTGGATCAACGACAAGCTGCTGACATCTCAGGATGTC
  TCCTATGATGAAGCACGAAACCTTCACAATAAATGGCTAAAGCACCAGGCGTTTGTGGCA
  GAGCTGGCTTCCCATGAAGGGTGGCTAGAGAACATCGATGCGGAAGGAAAGCAGCTGATG
  GATGAGAAGCCCCAGTTTACAGCCCTGGTGTCCCAAAAGCTGGAAGCCCTGCACCGGCTC
  TGGGACGAGCTGCAGGCCACCACAAAGGAGAAGACCCAGCACCTCTCGGCTGCCAGGAGC
  TCCGACCTGCGCTTGCAGACCCATGCTGACCTCAACAAGTGGATCAGCGCCATGGAGGAC
  CAGCTGCGGTCAGACGACCCGGGCAAGGACCTGACCAGTGTCAATCGGATGTTGGCTAAG
  CTGAAGCGAGTGGAGGACCAAGTGAATGTGCGAAAAGAGGAGCTGGGGGAGCTGTTTGCC
  CAGGTGCCTTCAATGGGAGAGGAGGGAGGAGATGCAGACTTGAGCATCGAGAAGCGGTTC
  CTGGACCTCCTGGAACCCCTAGGAAGGAGGAAGAAGCAGCTGGAATCATCCAGAGCCAAG
  CTGCAGATCAGCCGGGACTTAGAGGATGAGACGCTTTGGGTGGAGGAGAGGCTGCCTCTG
  GCCCAGTCAGCCGACTATGGCACTAATCTGCAAACTGTGCAACTGTTCATGAAGAAGAAC
  CAGACACTGCAGAATGAGATTCTGGGCCATACGCCGCGGGTTGAGGATGTGCTGCAGAGA
  GGGCAGCAGCTGGTGGAGGCGGCGGAGATCGACTGCCAGGACCTTGAGGAGCGCCTGGGG
  CACCTGCAGAGCTCCTGGGACAGGCTGCGGGAGGCAGCGGCCGGGAGGCTGCAGCGACTG
  AGGGACGCCAACGAGGCACAGCAGTACTACCTGGATGCAGACGAGGCTGAGGCCTGGATT
  GGCGAGCAGGAGCTCTACGTCATCTCCGATGAGATCCCCAAGGATGAAGAGGGCGCCATT
  GTGATGCTGAAGCGACATTTGCGGCAGCAGCGTGCGGTGGAGGACTACGGCCGGAACATC
  AAGCAGCTGGCCAGCCGGGCCCAGGGCCTGCTGTCTGCAGGCCACCCTGAGGGGGAACAG
  ATCATCAGACTTCAGGGGCAAGTGGACAAGCACTACGCAGGGCTGAAGGACGTGGCGGAA
  GAGCGCAAGCGCAAGCTGGAGAACATGTACCACCTGTTCCAGCTCAAGCGGGAGACCGAC
  GACCTGGAGCAGTGGATTTCAGAAAAGGAGCTAGTGGCCTCTTCCCCGGAAATGGGGCAA
  GACTTTGACCACGTGACTCTTCTGCGGGACAAGTTCCGGGACTTTGCCCGGGAGACCGGG
  GCGATTGGGCAGGAGCGGGTGGACAATGTGAATGCCTTCATCGAGCGACTCATCGACGCG
  GGCCACAGCGAGGCGGCCACCATCGCCGAGTGGAAGGACGGGCTGAACGAGATGTGGGCA
  GACCTCCTGGAGCTCATTGACACGCGCATGCAGCTGCTGGCCGCCTCCTATGACCTGCAC
  CGCTACTTCTACACGGGTGCCGAGATCCTGGGCCTCATCGACGAGAAGCACCGCGAGCTG
  CCCGAGGACGTGGGGCTGGACGCCAGCACGGCCGAGTCCTTCCACCGGGTGCACACAGCC
  TTCGAGCGGGAGCTCCACCTGCTGGGTGTCCAGGTGCAGCAGTTCCAGGACGTGGCCACC
  CGTCTGCAGACAGCATATGCTGGGGAGAAGGCAGAGGCCATCCAGAACAAGGAGCAGGAG
  GTGTCTGCCGCGTGGCAGGCGCTGCTCGATGCCTGTGCCGGGCGCCGGACCCAGCTAGTG
  GACACGGCGGATAAATTCCGCTTCTTCAGCATGGCCCGTGACCTCCTCTCCTGGATGGAG
  AGCATCATCCGGCAGATCGAGACCCAGGAGAGGCCCAGGGATGTCTCCTCTGTGGAACTG
  CTCATGAAGTATCACCAGGGCATCAATGCAGAGATTGAAACCCGGAGCAAGAACTTCAGT
  GCCTGCCTGGAGCTTGGCGAGTCCCTGCTGCAGCGGCAGCACCAGGCCTCAGAGGAGATC
  CGCGAGAAACTGCAGCAGGTGATGTCCAGGAGGAAAGAGATGAATGAGAAGTGGGAAGCC
  CGCTGGGAGCGGCTCCGCATGTTGCTGGAGGTGTGCCAGTTCTCGAGGGATGCCTCTGTG
  GCTGAGGCGTGGCTGATTGCCCAGGAGCCCTACCTGGCCAGCGGGGACTTTGGACACACA
  GTGGACAGTGTGGAGAAGCTCATCAAGAGGCATGAGGCTTTTGAGAAGTCCACGGCCAGC
  TGGGCAGAGCGCTTTGCTGCCCTGGAGAAGCCCACCACGCTTGAGCTGAAAGAACGCCAG
  ATTGCAGAGAGACCCGCAGAGGAGACTGGGCCTCAAGAGGAGGAAGGCGAGACAGCAGGG
  GAGGCTCCAGTTTCCCACCATGCGGCCACCGAGAGAACGTCCCCGGGGGAAGAAGAGGGA
  ACGTGGCCTCAGAACCTGCAGCAGCCACCACCGCCGGGTCAGCACAAGGATGGGCAGAAA
  TCCACTGGGGATGAGAGGCCCACCACGGAGCCCCTCTTTAAGGTCCTAGATACGCCTCTG
  AGCGAGGGTGATGAGCCCGCAACGCTGCCGGCCCCGCGGGACCATGGGCAGAGTGTGCAG
  ATGGAAGGCTACCTGGGCCGCAAGCATGACCTGGAGGGGCCCAACAAGAAGGCTTCCAAC
  AGGTCCTGGAACAACCTGTACTGTGTGCTCAGGAACAGTGAGCTAACCTTCTACAAGGAT
  GCCAAGAACCTGGCCCTGGGGATGCCCTACCATGGGGAGGAACCCCTGGCCCTGAGACAT
  GCCATCTGTGAGATTGCTGCCAACTACAAGAAGAAGAAGCACGTCTTTAAGCTGAGGCTG
  AGTAATGGCAGCGAGTGGCTCTTCCATGGCAAGGATGAGGAGGAGATGCTGTCCTGGCTG
  CAGGGCGTGAGCACCGCCATCAACGAGTCCCAGAGCATCCGCGTCAAGGCGCAGAGCCTG
  CCCCTGCCTTCCCTCTCCGGCCCCGACGCCAGCCTCGGCAAGAAAGACAAGGAGAAGAGA
  TTCAGCTTCTTCCCCAAAAAGAAGTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001024858
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024858.1, NP_001020029.1
• RefSeq Size: 10064 bp
• RefSeq ORF: 6987 bp
• Locus ID: 6710
• Cytogenetics: 14q23.3
• Protein Families: Druggable Genome
• Gene Summary: 'This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).