WAVE 1 (WASF1) (NM_001024934) Human Untagged Clone

CAT#: SC302313

WASF1 (untagged)-Human WAS protein family, member 1 (WASF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WASF1
• Synonyms: NEDALVS; SCAR1; WAVE; WAVE1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene SC302313 ORF sequence for NM_001024934, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGCTAGTGAAAAGAAACATCGATCCTAGGCACTTGTGCCACACAGCACTGCCTAGAGGCATTAAGA
  ATGAACTGGAATGTGTAACCAATATTTCCTTGGCAAATATAATTAGACAACTAAGTAGCCTAAGTAAATA
  TGCTGAAGATATATTTGGAGAATTATTCAATGAAGCACATAGTTTTTCCTTCAGAGTCAACTCATTGCAA
  GAACGTGTGGACCGTTTATCTGTTAGTGTTACACAGCTTGATCCAAAGGAAGAAGAATTGTCTTTGCAAG
  ATATAACAATGAGGAAAGCTTTCCGAAGTTCTACAATTCAAGACCAGCAGCTTTTCGATCGCAAGACTTT
  GCCTATTCCATTACAGGAGACGTACGATGTTTGTGAACAGCCTCCACCTCTCAATATACTCACTCCTTAT
  AGAGATGATGGTAAAGAAGGTCTGAAGTTTTATACCAATCCTTCGTATTTCTTTGATCTATGGAAAGAAA
  AAATGTTGCAAGATACAGAGGATAAGAGGAAGGAAAAGAGGAAGCAGAAGCAGAAAAATCTAGATCGTCC
  TCATGAACCAGAAAAAGTGCCAAGAGCACCTCATGACAGGCGGCGAGAATGGCAGAAGCTGGCCCAAGGT
  CCAGAGCTGGCTGAAGATGATGCTAATCTCTTACATAAGCATATTGAAGTTGCTAATGGCCCAGCCTCTC
  ATTTTGAAACAAGACCTCAGACATACGTGGATCATATGGATGGATCTTACTCACTTTCTGCCTTGCCATT
  TAGTCAGATGAGTGAGCTTCTGACTAGAGCTGAGGAAAGGGTATTAGTCAGACCACATGAACCACCTCCA
  CCTCCACCAATGCATGGAGCAGGAGATGCAAAACCGATACCCACCTGTATCAGTTCTGCTACAGGTTTGA
  TAGAAAATCGCCCTCAGTCACCAGCTACAGGCAGAACACCTGTGTTTGTGAGCCCCACTCCCCCACCTCC
  TCCACCACCTCTTCCATCTGCCTTGTCAACTTCCTCATTAAGAGCTTCAATGACTTCAACTCCTCCCCCT
  CCAGTACCTCCCCCACCTCCACCTCCAGCCACTGCTTTGCAAGCTCCAGCAGTACCACCACCTCCAGCTC
  CTCTTCAGATTGCCCCTGGAGTTCTTCACCCAGCTCCTCCTCCAATTGCACCTCCTCTAGTACAGCCCTC
  TCCACCAGTAGCTAGAGCTGCCCCAGTATGTGAGACTGTACCAGTTCATCCACTCCCACAAGGTGAAGTT
  CAGGGGCTGCCTCCACCCCCACCACCGCCTCCTCTGCCTCCACCTGGCATTCGACCATCATCACCTGTCA
  CAGTTACAGCTCTTGCTCATCCTCCCTCTGGGCTACATCCAACTCCATCTACTGCCCCAGGTCCCCATGT
  TCCATTAATGCCTCCATCTCCTCCATCACAAGTTATACCTGCTTCTGAGCCAAAGCGCCATCCATCAACC
  CTACCTGTAATCAGTGATGCCAGGAGTGTGCTACTGGAAGCAATACGAAAAGGTATTCAGCTACGCAAAG
  TAGAAGAGCAGCGTGAACAGGAAGCTAAGCATGAACGCATTGAAAACGATGTTGCCACCATCCTGTCTCG
  CCGTATTGCTGTTGAATATAGTGATTCGGAAGATGATTCAGAATTTGATGAAGTAGATTGGTTGGAGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024934
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024934.1, NP_001020105.1
• RefSeq Size: 3132 bp
• RefSeq ORF: 1680 bp
• Locus ID: 8936
• Cytogenetics: 6q21
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Gene Summary: The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an exon in the 5' UTR, as compared to variant 1. Variants 1-4 encode the same protein.