DHODH (NM_001025193) Human Untagged Clone

CAT#: SC302359

DHODH (untagged)-Human dihydroorotate dehydrogenase (DHODH), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHODH
• Synonyms: DHOdehase; dihydroorotate dehydrogenase; dihydroorotate oxidase; human complement of yeast URA1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001025193 edited
  ATGGCGTGGAGACACCTGCAAAAGCGGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGA
  GGACTTCTCTTCGCCTCCTACCTGATGGCCACGGGAGATGAGCGTTTCTATGCTGAACAC
  CTGATGCCGACTCTGCAGGGGCTGCTGGACCCGGAGTCAGCCCACAGACTGGCTGTTCGC
  TTCACCTCCCTGGGGCTCCTTCCACGGGCCAGATTTCAAGACTCTGACATGCTGGAAGTG
  AGAGTTCTGGGCCATAAATTCCGAAATCCAGTAGGAATTGCTGCAGGATTTGACAAGCAT
  GGGGAAGCCGTGGACGGACTTTATAAGATGGGCTTTGGTTTTGTTGAGATAGGAAGTGTG
  ACTCCAAAACCTCAGGAAGGAAACCCTAGACCCAGAGTCTTCCGCCTCCCTGAGGACCAA
  GCTGTCATTAACAGGTATGGATTTAACAGTCACGGGCTTTCAGTGGTGGAACACAGGTTA
  CGGGCCAGACAGCAGAAGCAGGCCAAGCTCACAGAAGTTGGGCATCGATGGGCTGATTGT
  TACGAACACCACCGTGAGTCGCCCTGCGGGCCTCCAGGGTGCCCTGCGCTCTGAAACAGG
  AGGGCTGAGTGGGAAGCCCCTCCGGGATTTATCAACTCAAACCATTCGGGAGATGTATGC
  ACTCACCCAAGGCCGAGTTCCCATAATTGGGGTTGGTGGTGTGAGCAGCGGGCAGGACGT
  GCTGGAGAAGATCCGGGCAGGGGCCTCCCTGGTGCAGCTGTACACGGCCCTCACCTTCTG
  GGGGCCACCCGTTGTGGGCAAAGTCAAGCGGGAACTGGAGGCCCTTCTGAAAGAGCAGGG
  CTTTGGCGGAGTCACAGATGCCATTGGAGCAGATCATCGGAGGTGAGGACAGCGTCTGAC
  GGGAAGCCTGATCTGGAACCTTCCCAAGGACTCAGGCAAGCCTTTGTGGCTGGATCATGA
  GAGGAGGGACTCCATCTTGAGCCATGTCCCCCAGCCATGGCATGGCTGCACTGTAAACGC
  CAATCGGGGGGTCACCAGGATCAACCGCAGGCTTTCTTCAGTCCCTTGGTCAGACCATAA
  ACTGCATTTTTGATTCTTTGTGGATTCAAACCCTAGGATCCATCAGTCTTGCAAGGACAT
  TGAATATTAGGAGGAAAAAGTCATGGAAAAAATAAAGCCATTTAGAACCTGGGAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001025193 unedited
  GGACGTCATATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAAAGGCGTGG
  AGACACCTGCAAAACGGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGAGGACTTCTCT
  TCGCCTCCTACCTGATGGCCACGGGAGATGAGCGTTTCTATGCTGAACACCTGATGCCGA
  CTCTGCAGGGGCTGCTGGACCCGGAGTCAGCCCACAGACTGGCTGTTCGCTTCACCTCCC
  TGGGGCTCCTTCCACGGGCCAGATTTCAAGACTCTGACATGCTGGAAGTGAGAGTTCTGG
  GCCATAAATTCCGAAATCCAGTAGGAATTGGCTGCAGGATTTGACAAGCATGGGGAAGCC
  GTGGACGGACTTTATAAGATGGGCTTNNTTGGGTTTTGTTGAGATAGGAAGTGTGACTCC
  AAAACCTCAGGAAGGAAACCCTAGACCCAGAGTCTTCCGCCTCCCTGAGGACCAAGCTGT
  CATTAACAGGTATGGATTTAACAGTCACGGGCTTTCAGTGGTGGAACACAGGTTACGGGC
  CAGACAGCAGAAGCAGGCCAAGCTCACAGAAGTTGGGCATCGATGGGCTGATTGTTACGA
  ACACCACCGTGAGTCGCCCTGCGGGCCTCCAGGGTGCCCTGCGCTCTGAAACAGGAGGGC
  TGAGTGGGAAGCCCCTCCGGGATTTATCAACTCAAACCATTCGGGAGATGTATGCACTCA
  CCCAAGGCCGAGTTCCCATAATTGGGGTTGGGTGGTGTGAGCAGCGGGCANGACGTGCTG
  GAGAAGATCCGGGCAGGGGCCTNCCTGGTGCAGCTGTACACGGCCCTCACCTTCTGGGGG
  CCACCGTTGTGGGCAAAGTCAAGCGGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_001025193
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acids.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025193.1, NP_001020364.1
• RefSeq Size: 1778 bp
• RefSeq ORF: 594 bp
• Locus ID: 1723
• Cytogenetics: 16q22.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Pyrimidine metabolism
• Gene Summary: 'The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]'