RFX5 (NM_001025603) Human Untagged Clone

CAT#: SC302426

RFX5 (untagged)-Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFX5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001025603, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGAAGATGAGCCTGATGCTAAGAGCCCCAAGACTGGGGGAAGGGCCCCCCCAGGTGGTGCTGAGG
  CTGGGGAACCTACCACCCTTCTTCAGAGGCTCCGAGGTACCATTTCCAAGGCCGTGCAGAACAAAGTAGA
  GGGGATCCTGCAAGATGTACAGAAATTTTCTGACAATGACAAGCTGTATCTCTACCTTCAGCTCCCCTCA
  GGACCCACCACTGGAGACAAAAGCTCAGAGCCAAGTACACTGAGCAATGAGGAGTACATGTATGCCTATA
  GGTGGATCCGCAACCACCTGGAAGAGCACACTGACACCTGTCTGCCAAAGCAAAGTGTTTATGATGCCTA
  TCGGAAGTACTGTGAGAGTCTTGCCTGTTGCCGCCCACTCAGCACAGCCAACTTTGGCAAGATCATCAGA
  GAGATCTTCCCTGACATCAAAGCTCGAAGGCTTGGTGGCCGGGGCCAGTCCAAATATTGCTACAGTGGCA
  TAAGGAGGAAGACCTTGGTGTCTATGCCACCCCTGCCTGGACTTGACCTAAAGGGTTCTGAGAGTCCAGA
  AATGGGCCCAGAAGTAACCCCAGCACCTCGAGATGAACTGGTGGAGGCAGCGTGTGCCCTGACCTGTGAC
  TGGGCAGAGCGGATCCTGAAACGGTCCTTCAGTTCCATCGTTGAGGTCGCCCGCTTCCTGCTACAGCAGC
  ATCTCATCTCTGCCCGATCTGCACATGCCCATGTGCTTAAGGCCATGGGGCTTGCTGAAGAGGACGAACA
  TGCACCTCGGGAACGGTCATCTAAACCAAAGAATGGTTTAGAGAACCCAGAGGGTGGAGCCCACAAGAAG
  CCAGAGAGACTGGCCCAGCCTCCTAAGGATCTGGAAGCCCGAACTGGGGCCGGTCCTCTCGCACGTGGAG
  AGCGGAAGAAGAGTGTAGTTGAGAGCTCGGCCCCAGGAGCCAATAACCTGCAGGTTAATGCCCTAGTGGC
  TCGGCTGCCTCTGCTCCTTCCCCGGGCCCCTCGCTCACTAATTCCGCCAATCCCAGTCTCTCCACCTATT
  CTGGCCCCCAGGCTTTCTTCAGGTGCCCTGAAAGTGGCTACACTGCCTCTGTCTAGTAGGGCCGGGGCAC
  CCCCAGCAGCTGTGCCCATCATTAACATGATCTTACCAACTGTTCCTGCTTTGCCTGGACCTGGACCTGG
  GCCTGGGCGAGCTCCACCTGGGGGACTCACTCAGCCCCGGGGCACAGAGAACAGAGAGGTAGGCATAGGT
  GGTGACCAAGGACCACATGACAAGGGTGTCAAGAGGACAGCTGAAGTACCTGTGAGTGAGGCCAGTGGGC
  AGGCTCCACCAGCTAAAGCAGCAAAGCAGGATATAGAGGATACAGCAAGTGATGCCAAAAGGAAACGGGG
  GCGCCCTCGAAAAAAGTCAGGTGGAAGTGGGGAAAGGAATTCTACCCCTCTCAAGTCAGCAGCTGCCATG
  GAATCTGCCCAGTCCTCAAGGTTACCATGGGAGACATGGGGCTCAGGAGGGGAAGGCAACTCAGCTGGAG
  GGGCAGAGAGGCCAGGGCCAATGGGAGAGGCTGAAAAGGGGGCAGTACTTGCCCAGGGTCAGGGAGATGG
  TACTGTTTCCAAAGGAGGAAGGGGCCCCGGTTCCCAGCATACCAAAGAAGCAGAAGATAAAATTCCCTTG
  GTCCCCTCAAAAGTGAGTGTCATCAAGGGCAGCAGAAGCCAAAAGGAGGCTTTTCCTTTGGCAAAGGGAG
  AGGTAGACACTGCACCACAGGGTAATAAAGACTTAAAGGAGCATGTGCTTCAAAGTTCCTTATCCCAGGA
  GCATAAAGACCCAAAAGCAACACCCCCATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025603
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025603.1, NP_001020774.1
• RefSeq Size: 3611 bp
• RefSeq ORF: 1851 bp
• Locus ID: 5993
• Cytogenetics: 1q21.3
• Protein Families: Transcription Factors
• Protein Pathways: Antigen processing and presentation, Primary immunodeficiency
• Gene Summary: 'A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.