PDE8B (NM_001029852) Human Untagged Clone

CAT#: SC302439

PDE8B (untagged)-Human phosphodiesterase 8B (PDE8B), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE8B
• Synonyms: ADSD; PPNAD3
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001029852, the custom clone sequence may differ by one or more nucleotides
  ATGGGCTGCGCCCCCAGCATCCATGTCTCGCAGAGCGGCGTGATCTACTGCCGGGACTCG
  GACGAGTCCAGCTCGCCCCGCCAGACCACCAGCGTGTCGCAGGGCCCGGCGGCACCCCTG
  CCCGGCCTCTTCGTCCAGACCGACGCCGCCGACGCCATCCCCCCGAGCCGCGCGTCGGGA
  CCCCCCAGCGTAGCCCGCGTCCGCAGGGCCCGCACCGAGCTGGGCAGCGGTAGCAGCGCG
  GGTTCCGCAGCCCCCGCCGCGACCACCAGCAGGGGCCGGAGGCGCCACTGCTGCAGCAGC
  GCCGAGGCCGAGACTCAGACCTGCTACACCAGCGTGAAGCAGGTGTCTTCTGCGGAGGTG
  CGCATCGGGCCCATGAGACTGACGCAGGACCCTATTCAGGTTTTGCTGATCTTTGCAAAG
  GAAGATAGTCAGAGCGATGGCTTCTGGTGGGCCTGCGACAGAGCTGGTTATAGATGCAAT
  ATTGCTCGGACTCCAGAGTCAGCCCTTGAATGCTTTCTTGATAAGCATCATGAAATTATT
  GTAATTGATCATAGACAAACTCAGAACTTCGATGCAGAAGCAGTGTGCAGGTCGATCCGG
  GCCACAAATCCCTCCGAGCACACGGTGATCCTCGCAGTGGTTTCGCGAGTATCGGATGAC
  CATGAAGAGGCGTCAGTCCTTCCTCTTCTCCACGCAGGCTTCAACAGGAGATTTATGGAG
  AATAGCAGCATAATTGCTTGCTATAATGAACTGATTCAAATAGAACATGGGGAAGTTCGC
  TCCCAGTTCAAATTACGGGCCTGTAATTCAGTGTTTACAGCATTAGATCACTGTCATGAA
  GCCATAGAAATAACAAGCGATGACCACGTGATTCAGTATGTCAACCCAGCCTTCGAAAGG
  ATGATGGGCTACCACAAAGGTGAGCTCCTGGGAAAAGAACTCGCTGATCTGCCCAAAAGC
  GATAAGAACCGGGCAGACCTTCTCGACACCATCAATACATGCATCAAGAAGGGAAAGGAG
  TGGCAGGGGGTTTACTATGCCAGACGGAAATCCGGGGACAGCATCCAACAGCACGTGAAG
  ATCACCCCAGTGATTGGCCAAGGAGGGAAAATTAGGCATTTTGTCTCGCTCAAGAAACTG
  TGTTGTACCACTGACAATAATAAGCAGATTCACAAGATTCATCGTGATTCAGGAGACAAT
  TCTCAGACAGAGCCTCATTCATTCAGATATAAGAACAGGAGGAAAGAGTCCATTGACGTG
  AAATCGATATCATCTCGAGGCAGTGATGCACCAAGCCTGCAGAATCGTCGCTATCCGTCC
  ATGGCGAGGATCCACTCCATGACCATCGAGGCTCCCATCACAAAGGACGGCTTGAGAAGA
  CTGTCAGGAAACGAGTATGTGTTTACTAAGAATGTGCACCAGAGTCACAGTCACCTTGCA
  ATGCCAATAACCATCAATGATGTTCCCCCTTGTATCTCTCAATTACTTGATAATGAGGAG
  AGTTGGGACTTCAACATCTTTGAATTGGAAGCCATTACGCATAAAAGGCCATTGGTTTAT
  CTGGGCTTAAAGGTCTTCTCTCGGTTTGGAGTATGTGAGTTTTTAAACTGTTCTGAAACC
  ACTCTTCGGGCCTGGTTCCAAGTGATCGAAGCCAACTACCACTCTTCCAATGCCTACCAC
  AACTCCACCCATGCTGCCGACGTCCTGCACGCCACCGCTTTCTTTCTTGGAAAGGAAAGA
  GTAAAGGGAAGCCTCGATCAGTTGGATGAGGTGGCAGCCCTCATTGCTGCCACAGTCCAT
  GACGTGGATCACCCGGGAAGGACCAACTCTTTCCTCTGCAATGCAGGCAGTGAGCTTGCT
  GTGCTCTACAATGACACTGCTGTTCTGGAGAGTCACCACACCGCCCTGGCCTTCCAGCTC
  ACGGTCAAGGACACCAAATGCAACATTTTCAAGAATATTGACAGGAACCATTATCGAACG
  CTGCGCCAGGCTATTATTGACATGGTTTTGGCAACAGAGATGACAAAACACTTTGAACAT
  GTGAATAAGTTTGTGAACAGCATCAACAAGCCAATGGCAGCTGAGATTGAAGGCAGCGAC
  TGTGAATGCAACCCTGCTGGGAAGAACTTCCCTGAAAACCAAATCCTGATCAAACGCATG
  ATGATTAAGTGTGCTGACGTGGCCAACCCATGCCGCCCCTTGGACCTGTGCATTGAATGG
  GCTGGGAGGATCTCTGAGGAGTATTTTGCACAGACTGATGAAGAGAAGAGACAGGGACTA
  CCTGTGGTGATGCCAGTGTTTGACCGGAATACCTGTAGCATCCCCAAGTCTCAGATCTCT
  TTCATTGACTACTTCATAACAGACATGTTTGATGCTTGGGATGCCTTTGCACATCTGCCA
  GCCCTGATGCAACATTTGGCTGACAACTACAAACACTGGAAGACACTAGATGACCTAAAG
  TGCAAAAGTTTGAGGCTTCCATCTGACAGCTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001029852
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029852.1, NP_001025023.1
• RefSeq Size: 3405 bp
• RefSeq ORF: 2493 bp
• Locus ID: 8622
• Cytogenetics: 5q13.3
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: The protein encoded by this gene is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (5), also known as PDE8B3, lacks an alternate in-frame exon compared to variant 1. The encoded isoform (5) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.