PDE8B (NM_001029853) Human Untagged Clone

CAT#: SC302440

PDE8B (untagged)-Human phosphodiesterase 8B (PDE8B), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE8B
• Synonyms: ADSD; PPNAD3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001029853, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCTGCGCCCCCAGCATCCATGTCTCGCAGAGCGGCGTGATCTACTGCCGGGACTCGGACGAGTCCA
  GCTCGCCCCGCCAGACCACCAGCGTGTCGCAGGGCCCGGCGGCACCCCTGCCCGGCCTCTTCGTCCAGAC
  CGACGCCGCCGACGCCATCCCCCCGAGCCGCGCGTCGGGACCCCCCAGCGTAGCCCGCGTCCGCAGGGCC
  CGCACCGAGCTGGGCAGCGGTAGCAGCGCGGGTTCCGCAGCCCCCGCCGCGACCACCAGCAGGGGCCGGA
  GGCGCCACTGCTGCAGCAGCGCCGAGGCCGAGACTCAGACCTGCTACACCAGCGTGAAGGTTTTGCTGAT
  CTTTGCAAAGGAAGATAGTCAGAGCGATGGCTTCTGGTGGGCCTGCGACAGAGCTGGTTATAGATGCAAT
  ATTGCTCGGACTCCAGAGTCAGCCCTTGAATGCTTTCTTGATAAGCATCATGAAATTATTGTAATTGATC
  ATAGACAAACTCAGAACTTCGATGCAGAAGCAGTGTGCAGGTCGATCCGGGCCACAAATCCCTCCGAGCA
  CACGGTGATCCTCGCAGTGGTTTCGCGAGTATCGGATGACCATGAAGAGGCGTCAGTCCTTCCTCTTCTC
  CACGCAGGCTTCAACAGGAGATTTATGGAGAATAGCAGCATAATTGCTTGCTATAATGAACTGATTCAAA
  TAGAACATGGGGAAGTTCGCTCCCAGTTCAAATTACGGGCCTGTAATTCAGTGTTTACAGCATTAGATCA
  CTGTCATGAAGCCATAGAAATAACAAGCGATGACCACGTGATTCAGTATGTCAACCCAGCCTTCGAAAGG
  ATGATGGGCTACCACAAAGGTGAGCTCCTGGGAAAAGAACTCGCTGATCTGCCCAAAAGCGATAAGAACC
  GGGCAGACCTTCTCGACACCATCAATACATGCATCAAGAAGGGAAAGGAGTGGCAGGGGGTTTACTATGC
  CAGACGGAAATCCGGGGACAGCATCCAACAGCACGTGAAGATCACCCCAGTGATTGGCCAAGGAGGGAAA
  ATTAGGCATTTTGTCTCGCTCAAGAAACTGTGTTGTACCACTGACAATAATAAGCAGATTCACAAGATTC
  ATCGTGATTCAGGAGACAATTCTCAGACAGAGCCTCATTCATTCAGATATAAGAACAGGAGGAAAGAGTC
  CATTGACGTGAAATCGATATCATCTCGAGGCAGTGATGCACCAAGCCTGCAGAATCGTCGCTATCCGTCC
  ATGGCGAGGATCCACTCCATGACCATCGAGGCTCCCATCACAAAGGTTATAAATATAATCAATGCAGCCC
  AAGAAAACAGCCCAGTCACAGTAGCGGAAGCCTTGGACAGAGTTCTAGAGATTTTACGGACCACAGAACT
  GTACTCCCCTCAGCTGGGTACCAAAGATGAAGATCCCCACACCAGTGATCTTGTTGGAGGCCTGATGACT
  GACGGCTTGAGAAGACTGTCAGGAAACGAGTATGTGTTTACTAAGAATGTGCACCAGAGTCACAGTCACC
  TTGCAATGCCAATAACCATCAATGATGTTCCCCCTTGTATCTCTCAATTACTTGATAATGAGGAGAGTTG
  GGACTTCAACATCTTTGAATTGGAAGCCATTACGCATAAAAGGCCATTGGTTTATCTGGGCTTAAAGGTC
  TTCTCTCGGTTTGGAGTATGTGAATTTTTAAACTGTTCTGAAACCACTCTTCGGGCCTGGTTCCAAGTGA
  TCGAAGCCAACTACCACTCTTCCAATGCCTACCACAACTCCACCCATGCTGCCGACGTCCTGCACGCCAC
  CGCTTTCTTTCTTGGAAAGGAAAGAGTAAAGGGAAGCCTCGATCAGTTGGATGAGGTGGCAGCCCTCATT
  GCTGCCACAGTCCATGACGTGGATCACCCGGGAAGGACCAACTCTTTCCTCTGCAATGCAGGCAGTGAGC
  TTGCTGTGCTCTACAATGACACTGCTGTTCTGGAGAGTCACCACACCGCCCTGGCCTTCCAGCTCACGGT
  CAAGGACACCAAATGCAACATTTTCAAGAATATTGACAGGAACCATTATCGAACGCTGCGCCAGGCTATT
  ATTGACATGGTTTTGGCAACAGAGATGACAAAACACTTTGAACATGTGAATAAGTTTGTGAACAGCATCA
  ACAAGCCAATGGCAGCTGAGATTGAAGGCAGCGACTGTGAATGCAACCCTGCTGGGAAGAACTTCCCTGA
  AAACCAAATCCTGATCAAACGCATGATGATTAAGTGTGCTGACGTGGCCAACCCATGCCGCCCCTTGGAC
  CTGTGCATTGAATGGGCTGGGAGGATCTCTGAGGAGTATTTTGCACAGACTGATGAAGAGAAGAGACAGG
  GACTACCTGTGGTGATGCCAGTGTTTGACCGGAATACCTGTAGCATCCCCAAGTCTCAGATCTCTTTCAT
  TGACTACTTCATAACAGACATGTTTGATGCTTGGGATGCCTTTGCACATCTGCCAGCCCTGATGCAACAT
  TTGGCTGACAACTACAAACACTGGAAGACACTAGATGACCTAAAGTGCAAAAGTTTGAGGCTTCCATCTG
  ACAGCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001029853
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029853.2, NP_001025024.1
• RefSeq Size: 4345 bp
• RefSeq ORF: 2598 bp
• Locus ID: 8622
• Cytogenetics: 5q13.3
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: The protein encoded by this gene is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (4) lacks an alternate in-frame exon compared to variant 1. The encoded isoform (4) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.