Melanopsin (OPN4) (NM_001030015) Human Untagged Clone

CAT#: SC302494

OPN4 (untagged)-Human opsin 4 (OPN4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OPN4
• Synonyms: MOP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001030015, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACCCTCCTTCGGGGCCAAGAGTCCCGCCCAGCCCAACCCAAGAGCCCAGCTGCATGGCCACCCCAG
  CACCACCCAGCTGGTGGGACAGCTCCCAGAGCAGCATCTCCAGCCTGGGCCGGCTTCCATCCATCAGTCC
  CACAGCACCTGGGACTTGGGCTGCTGCCTGGGTCCCCCTCCCCACGGTTGATGTTCCAGACCATGCCCAC
  TATACCCTGGGCACAGTGATCTTGCTGGTGGGACTCACGGGGATGCTGGGCAACCTGACGGTCATCTATA
  CCTTCTGCAGAGCTGTGCTTCGTGGAGTCACTGTGATGATGCAGAGCAGAAGCCTCCGGACACCTGCCAA
  CATGTTCATTATCAACCTCGCGGTCAGCGACTTCCTCATGTCCTTCACCCAGGCCCCTGTCTTCTTCACC
  AGTAGCCTCTATAAGCAGTGGCTCTTTGGGGAGACAGGCTGCGAGTTCTATGCCTTCTGTGGAGCTCTCT
  TTGGCATTTCCTCCATGATCACCCTGACGGCCATCGCCCTGGACCGCTACCTGGTAATCACACGCCCGCT
  GGCCACCTTTGGTGTGGCGTCCAAGAGGCGTGCGGCATTTGTCCTGCTGGGCGTTTGGCTCTATGCCCTG
  GCCTGGAGTCTGCCACCCTTCTTCGGCTGGAGCGCCTACGTGCCCGAGGGGTTGCTGACATCCTGCTCCT
  GGGACTACATGAGCTTCACGCCGGCCGTGCGTGCCTACACCATGCTTCTCTGCTGCTTCGTGTTCTTCCT
  CCCTCTGCTTATCATCATCTACTGCTACATCTTCATCTTCAGGGCCATCCGGGAGACAGGACGGGCTCTC
  CAGACCTTCGGGGCCTGCAAGGGCAATGGCGAGTCCCTGTGGCAGCGGCAGCGGCTGCAGAGCGAGTGCA
  AGATGGCCAAGATCATGCTGCTGGTCATCCTCCTCTTCGTGCTCTCCTGGGCTCCCTATTCCGCTGTGGC
  CCTGGTGGCCTTTGCTGGGTACGCACACGTCCTGACACCCTACATGAGCTCGGTGCCAGCCGTCATCGCC
  AAGGCCTCTGCAATCCACAACCCCATCATTTACGCCATCACCCACCCCAAGTACAGGGTGGCCATTGCCC
  AGCACCTGCCCTGCCTGGGGGTGCTGCTGGGTGTATCACGCCGGCACAGTCGCCCCTACCCCAGCTACCG
  CTCCACCCACCGCTCCACGCTGACCAGCCACACCTCCAACCTCAGCTGGATCTCCATACGGAGGCGCCAG
  GAGTCCCTGGGCTCGGAGAGTGAGGTGGGCTGGACACACATGGAGGCAGCAGCTGTGTGGGGAGCTGCCC
  AGCAAGCAAATGGGCGGTCCCTCTACGGTCAGGGTCTGGAGGACTTGGAAGCCAAGGCACCCCCCAGACC
  CCAGGGACACGAAGCAGAGACTCCAGGGAAGACCAAGGGGCTGATCCCCAGCCAGGACCCCAGGATGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001030015
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030015.2, NP_001025186.1
• RefSeq Size: 2419 bp
• RefSeq ORF: 1470 bp
• Locus ID: 94233
• Cytogenetics: 10q23.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) includes an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in a longer protein (isoform 2) compared to isoform 1.