RUNX3 (NM_001031680) Human Untagged Clone

CAT#: SC302527

RUNX3 (untagged)-Human runt-related transcription factor 3 (RUNX3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RUNX3
• Synonyms: AML2; CBFA3; PEBP2aC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001031680, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCATCGAACAGCATCTTCGACTCCTTCCCGACCTACTCGCCGACCTTCATCCGCGACCCAAGCACCA
  GCCGCCGCTTCACACCTCCCTCCCCGGCCTTCCCCTGCGGCGGCGGCGGCGGCAAGATGGGCGAGAACAG
  CGGCGCGCTGAGCGCGCAGGCGGCCGTGGGGCCCGGAGGGCGCGCCCGGCCCGAGGTGCGCTCGATGGTG
  GACGTGCTGGCGGACCACGCAGGCGAGCTCGTGCGCACCGACAGCCCCAACTTCCTCTGCTCCGTGCTGC
  CCTCGCACTGGCGCTGCAACAAGACGCTGCCCGTCGCCTTCAAGGTGGTGGCATTGGGGGACGTGCCGGA
  TGGTACGGTGGTGACTGTGATGGCAGGCAATGACGAGAACTACTCCGCTGAGCTGCGCAATGCCTCGGCC
  GTCATGAAGAACCAGGTGGCCAGGTTCAACGACCTTCGCTTCGTGGGCCGCAGTGGGCGAGGGAAGAGTT
  TCACCCTGACCATCACTGTGTTCACCAACCCCACCCAAGTGGCGACCTACCACCGAGCCATCAAGGTGAC
  CGTGGACGGACCCCGGGAGCCCAGACGGCACCGGCAGAAGCTGGAGGACCAGACCAAGCCGTTCCCTGAC
  CGCTTTGGGGACCTGGAACGGCTGCGCATGCGGGTGACACCGAGCACACCCAGCCCCCGAGGCTCACTCA
  GCACCACAAGCCACTTCAGCAGCCAGCCCCAGACCCCAATCCAAGGCACCTCGGAACTGAACCCATTCTC
  CGACCCCCGCCAGTTTGACCGCTCCTTCCCCACGCTGCCAACCCTCACGGAGAGCCGCTTCCCAGACCCC
  AGGATGCATTATCCCGGGGCCATGTCAGCTGCCTTCCCCTACAGCGCCACGCCCTCGGGCACGAGCATCA
  GCAGCCTCAGCGTGGCGGGCATGCCGGCCACCAGCCGCTTCCACCATACCTACCTCCCGCCACCCTACCC
  GGGGGCCCCGCAGAACCAGAGCGGGCCCTTCCAGGCCAACCCGTCCCCCTACCACCTCTACTACGGGACA
  TCCTCTGGCTCCTACCAGTTCTCCATGGTGGCCGGCAGCAGCAGTGGGGGCGACCGCTCACCTACCCGCA
  TGCTGGCCTCTTGCACCAGCAGCGCTGCCTCTGTCGCCGCCGGCAACCTCATGAACCCCAGCCTGGGCGG
  CCAGAGTGATGGCGTGGAGGCCGACGGCAGCCACAGCAACTCACCCACGGCCCTGAGCACGCCAGGCCGC
  ATGGATGAGGCCGTGTGGCGGCCCTACTGA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_001031680
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031680.1, NP_001026850.1
• RefSeq Size: 4340 bp
• RefSeq ORF: 1290 bp
• Locus ID: 864
• Cytogenetics: 1p36.11
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]'
  Transcript Variant: This variant (1), also known as p46, represents the longest transcript and encodes the longer isoform (1). Variants 1 and 3 encode the same protein.