LIM kinase 2 (LIMK2) (NM_001031801) Human Untagged Clone

CAT#: SC302595

LIMK2 (untagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIMK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001031801, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGAGTTACTTGTCAGTCCCGGCTTACTTCACCTCCAGAGACCTGTTTCGGTGTTCAGAATGCCAGG
  ATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACTACTGGGGGAAGTT
  TGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGGCCTTTTATGGTGGCTGGGGAGTTCAAGTAC
  CACCCAGAGTGCTTTGCCTGTATGAGCTGCAAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGC
  AGCATGCCACCCTCTACTGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTC
  CACAGAGTCTGTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGC
  AGGCGGGGCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAAGTGAAAGAGG
  TCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTGGGGACCGCATCCTGGAGATCAA
  TGGGACCCCCGTCCGCACACTTCGAGTGGAGGAGGTGGAGGATGCAATTAGCCAGACGAGCCAGACACTT
  CAGCTGTTGATTGAACATGACCCCGTCTCCCAACGCCTGGACCAGCTGCGGCTGGAGGCCCGGCTCGCTC
  CTCACATGCAGAATGCCGGACACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGAC
  ACTGAGGAGACGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAGGAG
  CCCCTGCTGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGCTATTCACAGCAGA
  TCTTCCGGCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGT
  GACACACAAAGCCACGGGCAAAGTGATGGTCATGAAAGAGTTAATTCGATGTGATGAGGAGACCCAGAAA
  ACTTTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGC
  TGTACAAGGATAAGAAGCTGAACCTCCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCG
  CAGTATGGATCCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCCTAT
  TTGCACTCTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAGTTGGACAAGACTG
  TGGTGGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAAAGGGCCCCCATGGAGAAGGC
  CACCACCAAGAAACGCACCTTGCGCAAGAACGACCGCAAGAAGCGCTACACGGTGGTGGGAAACCCCTAC
  TGGATGGCCCCTGAGATGCTGAACGGAAAGAGCTATGATGAGACGGTGGATATCTTCTCCTTTGGGATCG
  TTCTCTGTGAGATCATTGGGCAGGTGTATGCAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCT
  CAACGTGAAGCTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCCGCC
  ATCTGCTGCAGACTGGAGCCTGAGAGCAGAGCCCCCCCCGGGGCCGCAGGAGAGGGCCCGGGCTGCGCGG
  ATGATGAGGGCCCAGTGAGGCGCCAAGGGAAGGTCACCATCAAGTATGACCCCAAGGAGCTACGGAAGCA
  CCTCAACCTAGAGGAGTGGATCCTGGAGCAGCTCACGCGCCTCTACGACTGCCAGGAAGAGGAGATCTCA
  GAACTAGAGATTGACGTGGATGAGCTCCTGGACATGGAGAGTGACGATGCCTGGGCTTCCAGGGTCAAGG
  AGCTGCTGGTTGACTGTTACAAACCCACAGAGGCCTTCATCTCTGGCCTGCTGGACAAGATCCGGGCCAT
  GCAGAAGCTGAGCACACCCCAGAAGAAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001031801
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031801.1, NP_001026971.1
• RefSeq Size: 2914 bp
• RefSeq ORF: 2061 bp
• Locus ID: 3985
• Cytogenetics: 22q12.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Gene Summary: 'There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).