TIAL1 (NM_001033925) Human Untagged Clone

CAT#: SC302779

TIAL1 (untagged)-Human TIA1 cytotoxic granule-associated RNA binding protein-like 1 (TIAL1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TIAL1
• Synonyms: TCBP; TIAR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001033925, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGGAAGACGACGGGCAGCCCCGGACTCTATACGTAGGTAACCTTTCCAGAGATGTGACAGAAGTCC
  TTATACTTCAGTTGTTCAGTCAGATTGGACCCTGTAAAAGCTGTAAAATGATAACAGAGCAACCCGATAG
  CAGAAGGGTCAACTCTTCTGTTGGATTTTCTGTTTTGCAGCATACAAGCAATGACCCATATTGCTTTGTG
  GAATTTTATGAACACAGAGATGCAGCTGCTGCATTAGCTGCTATGAATGGGAGAAAAATTTTGGGAAAGG
  AGGTCAAAGTAAACTGGGCAACCACACCAAGTAGCCAGAAAAAAGATACTTCCAATCACTTCCATGTGTT
  TGTTGGGGATTTGAGTCCAGAAATTACAACAGAAGATATCAAATCAGCATTTGCCCCCTTTGGTAAAATA
  TCGGATGCCCGGGTAGTTAAAGACATGGCAACTGGAAAATCCAAAGGCTATGGTTTTGTATCTTTTTATA
  ACAAACTGGATGCAGAAAATGCGATTGTGCATATGGGCGGTCAGTGGTTGGGTGGTCGTCAAATCCGAAC
  CAATTGGGCCACTCGTAAACCACCTGCACCTAAAAGTACACAAGAAAACAACACTAAGCAGTTGAGATTT
  GAAGATGTAGTAAACCAGTCAAGTCCAAAAAATTGTACTGTGTACTGTGGAGGAATTGCGTCTGGGTTAA
  CAGATCAGCTTATGAGACAGACATTCTCACCATTTGGACAAATTATGGAAATAAGAGTTTTCCCAGAAAA
  GGGCTATTCATTTGTCAGATTTTCAACCCATGAAAGTGCAGCCCATGCCATTGTTTCGGTGAACGGTACT
  ACGATTGAAGGACATGTGGTTAAATGCTATTGGGGTAAAGAATCTCCTGATATGACTAAAAACTTCCAAC
  AGGTTGACTATAGTCAATGGGGCCAATGGAGCCAAGTGTATGGAAACCCACAACAGTATGGACAGTATAT
  GGCAAATGGGTGGCAAGTACCGCCTTATGGAGTATACGGGCAACCATGGAATCAACAAGGATTTGGAGTA
  GATCAATCACCTTCTGCTGCTTGGATGGGTGGATTTGGTGCTCAGCCTCCCCAAGGACAAGCTCCTCCCC
  CTGTAATACCTCCTCCTAACCAAGCCGGATATGGTATGGCAAGTTACCAAACACAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033925
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033925.1, NP_001029097.1
• RefSeq Size: 3945 bp
• RefSeq ORF: 1179 bp
• Locus ID: 7073
• Cytogenetics: 10q26.11
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1, resulting in a longer protein (isoform 2).