Calcitonin (CALCA) (NM_001033953) Human Untagged Clone

CAT#: SC302782

CALCA (untagged)-Human calcitonin-related polypeptide alpha (CALCA), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CALCA
• Synonyms: CALC1; CGRP; CGRP-alpha; CGRP-I; CGRP1; CT; KC; PCT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001033953 edited
  CGAGATTCTGGCTCAGAGAGGTGTCATGGGCTTCCAAAAGTTCTCCCCCTTCCTGGCTCT
  CAGCATCTTGGTCCTGTTGCAGGCAGGCAGCCTCCATGCAGCACCATTCAGGTCTGCCCT
  GGAGAGCAGCCCAGCAGACCCGGCCACGCTCAGTGAGGACGAAGCGCGCCTCCTGCTGGC
  TGCACTGGTGCAGGACTATGTGCAGATGAAGGCCAGTGAGCTGGAGCAGGAGCAAGAGAG
  AGAGGGCTCCAGAATCATTGCCCAGAAGAGAGCCTGTGACACTGCCACCTGTGTGACTCA
  TCGGCTGGCAGGCTTGCTGAGCAGATCAGGGGGTGTGGTGAAGAACAACTTTGTGCCCAC
  CAATGTGGGTTCCAAAGCCTTTGGCAGGCGCCGCAGGGACCTTCAAGCCTGAGCAGCTGA
  ATGACTCAAGAAGGTCACAATAAAGCTGAACTCCTTTTAATGTGTAATGAAAGCAATTTG
  TAGGAAAGGCTCCATGGAAGACA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001033953 unedited
  GGGACGTTAGATTTGTATACGACTCATATAGGCGGCCGCGNATTCGCCCTTCGANATTCT
  GGCTCAGAGAGGTGTCATGGGCTTCCAAAGTTCTCCCCCTTCCTGGCTCTCAGCATCTTG
  GTCCTGTTGCAGGCAGGCAGCCTCCATGCAGCACCATTCAGGTCTGCCCTGGAGAGCAGC
  CCAGCAGACCCGGCCACGCTCAGTGAGGACGAAGCGCGCCTCCTGCTGGCTGCACTGGTG
  CAGGACTATGTGCAGATGAAGGCCAGTGAGCTGGAGCAGGAGCAAGAGAGAGAGGGCTCC
  AGAATCATTGCCCAGAAGAGAGCCTGTGACACTGCCACCTGTGTGACTCATCGGCTGGCA
  GGCTTGCTGAGCAGATCAGGGGGTGTGGTGAAGAACAACTTTGTGCCCACCAATGTGGGT
  TCCAAAGCCTTTGGCAGGCGCCGCAGGGACCTTCAAGCCTGAGCAGCTGAATGACTCAAG
  AAGGTCACAATAAAGCTGAACTCCTTTTAATGTGTAATGAAAGCAATTTGTAGGAAAGGC
  TCCATGGAAGACAAAGGGCGAATTCAGATCTGGTACCGATATCAAGCTTGTCGACTCTAG
  ATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCC
  TCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCNT
  ATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTATGGNGTGGA
  GGGGGGTGGTATGGAGCAGGGGCAAGTTGGGAAGACACCTGTAGGGCCTGCNGGGTCTAT
  TGGGAACCAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATC
  
• Restriction Sites: Please inquire
• ACCN: NM_001033953
• Insert Size: 500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033953.1, NP_001029125.1
• RefSeq Size: 915 bp
• RefSeq ORF: 387 bp
• Locus ID: 796
• Cytogenetics: 11p15.2
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]'
  Transcript Variant: This variant (3) represents the longest coding transcript and encodes the shorter protein, which is cleaved to yield calcitonin gene-related peptide 1 (alpha-CGRP). Alpha-CGRP functions both as an antimicrobial peptide and a vasodilator. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.