PHYH (NM_001037537) Human Untagged Clone

CAT#: SC302910

PHYH (untagged)-Human phytanoyl-CoA 2-hydroxylase (PHYH), transcript variant 2


  "NM_001037537" in other vectors (4)

Reconstitution Protocol

USD 420.00

3 Weeks*

Size
    • 10 ug

Product Images

Other products for "PHYH"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PHYH
Synonyms LN1; LNAP1; PAHX; PHYH1; RD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001037537, the custom clone sequence may differ by one or more nucleotides


ATGAGAGATGTGACCATTTCGAAATCCGAATATGCTCCAAGTGAGAAGATGATCACGAAGGTCCAGGATT
TCCAGGAAGATAAGGAGCTCTTCAGATACTGCACTCTCCCCGAGATTCTGAAATATGTGGAGTGCTTCAC
TGGACCTAATATTATGGCCATGCACACAATGTTGATAAACAAACCTCCAGATTCTGGCAAGAAGACGTCC
CGTCACCCCCTGCACCAGGACCTGCACTATTTCCCCTTCAGGCCCAGCGATCTCATCGTTTGCGCCTGGA
CGGCGATGGAGCACATCAGCCGGAACAACGGCTGTCTGGTTGTGCTCCCAGGCACACACAAGGGCTCCCT
GAAGCCCCACGATTACCCCAAGTGGGAGGGGGGAGTTAACAAAATGTTCCACGGGATCCAGGACTACGAG
GAAAACAAGGCCCGGGTGCACCTGGTGATGGAGAAGGGCGACACTGTTTTCTTCCATCCTTTGCTCATCC
ACGGATCTGGTCAGAATAAAACCCAGGGATTCCGGAAGGCAATTTCCTGCCATTTCGCCAGTGCCGATTG
CCACTACATTGACGTGAAGGGCACCAGTCAAGAAAACATCGAGAAGGAAGTTGTAGGAATAGCACATAAA
TTCTTTGGAGCTGAAAATAGCGTGAACTTGAAGGATATTTGGATGTTTCGAGCTCGACTTGTGAAAGGAG
AAAGAACCAATCTTTGA


Restriction Sites SgfI-MluI     
ACCN NM_001037537
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001037537.1, NP_001032626.1
RefSeq Size 1725 bp
RefSeq ORF 717 bp
Locus ID 5264
Cytogenetics 10p13
Protein Families Druggable Genome
Gene Summary This gene is a member of the PhyH family and encodes a peroxisomal protein that is involved in the alpha-oxidation of 3-methyl branched fatty acids. Specifically, this protein converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. Mutations in this gene have been associated with Refsum disease (RD) and deficient protein activity has been associated with Zellweger syndrome and rhizomelic chondrodysplasia punctata. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream ATG and result in an isoform (b) with a shorter N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b).

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.