GPR149 (NM_001038705) Human Untagged Clone

CAT#: SC302956

GPR149 (untagged)-Human G protein-coupled receptor 149 (GPR149)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR149
• Synonyms: IEDA; PGR10; R35
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001038705 edited
  ATGTCTTTATTTCTCAGTAACTTATCAACAAATGACTCTAGCCTGTGGAAAGAGAATCAT
  AATTCTACGGACCTTTTAAATCCGCCAGGAACCCTGAATATCTATCTTTTTTGCTTGACA
  TGTCTCATGACTTTTGCAGCCTTGGTGGGCAGCATTTATTCACTAATTTCCCTGCTGAAA
  ATGCAGAACAGAACTGTTGTGTCCATGCTTGTGGCTTCCTGGTCTGTGGATGATCTCATG
  AGCGTCCTGTCGGTGACCATCTTCATGTTTTTGCAGTGGCCAAACGAGGTCCCCGGTTAC
  TTCCAATTTCTGTGCACCACCTCTGCCTTAATGTATTTATGCCAGGGCCTCTCTAGCAAC
  TTGAAGGCGACTCTCCTAGTCTCTTACAACTTTTATACGATGCACAGAGGTGTGGGGAGC
  CAGACAGCCTCCAGAAGATCGGGCCAGGTGCTCGGCGTGGTGCTGACCGTGTGGGCAGCC
  AGTCTGCTGCTCTCGGCGCTCCCGCTGTGCGGCTGGGGCGCCTTCGTGCGCACGCCCTGG
  GGCTGCCTGGTGGACTGCTCCAGCTCCTACGTACTATTCCTCTCTATCGTGTACGCTTTG
  GCCTTCGGACTCCTCGTGGGCCTCTCAGTCCCACTCACTCACCGATTGCTGTGTTCGGAG
  GAGCCGCCGAGACTCCACTCCAACTACCAGGAAATTTCCCGTGGAGCTTCAATTCCTGGG
  ACCCCTCCTACTGCGGGGAGAGTGGTTTCCCTGTCCCCAGAGGATGCTCCAGGCCCGAGT
  CTGCGGCGCTCTGGGGGATGCTCTCCGAGCTCCGACACCGTGTTCGGACCGGGTGCGCCC
  GCTGCCGCTGGGGCTGAAGCCTGCAGGCGTGAGAACCGGGGGACTCTCTATGGCACCAGG
  AGCTTCACCGTGAGCGTAGCGCAGAAGCGCTTCGCTTTGATCCTAGCGCTTACAAAAGTC
  GTCCTTTGGCTGCCCATGATGATGCACATGGTGGTCCAGAACGTCGTGGGGTTTCAGAGC
  CTTCCCTTGGAGACATTCAGCTTTCTACTTACCCTGCTGGCCACCACTGTAACCCCAGTG
  TTTGTCTTGTCCAAACGCTGGACCCACTTGCCCTGTGGCTGCATCATCAACTGCAGGCAG
  AACGCATATGCAGTGGCGTCCGATGGGAAAAAAATCAAGAGAAAAGGCTTTGAATTCAAT
  CTATCATTCCAAAAAAGTTATGGGATTTATAAAATAGCACATGAAGATTACTATGATGAT
  GATGAAAATTCCATATTCTATCACAACCTGATGAACTCTGAGTGTGAAACTACAAAAGAC
  CCTCAGAGAGACAACCGTAACATCTTCAATGCTATAAAAGTAGAAATCAGCACCACGCCC
  TCTCTGGACAGCTCCACACAAAGAGGCATCAACAAATGCACAAATACTGATATTACAGAA
  GCTAAACAGGATTCCAACAACAAAAAGGATGCGTTTTCTGACAAAACAGGAGGTGATATT
  AACTATGAAGAAACTACCTTTTCTGAAGGGCCAGAAAGAAGACTGTCTCATGAAGAGAGT
  CAGAAACCAGATCTTTCAGACTGGGAGTGGTGTAGGAGTAAATCAGAAAGAACCCCTCGT
  CAGCGTTCCGGTTATGCCCTTGCCATTCCCTTGTGTGCATTCCAGGGGACTGTGTCTCTC
  CATGCACCTACAGGGAAAACCCTATCTCTTTCTACCTATGAGGTAAGCGCAGAAGGGCAA
  AAAATAACTCCAGCCTCTAAGAAAATAGAAGTCTATCGATCCAAAAGTGTTGGCCATGAA
  CCAAACTCAGAAGATTCTTCATCCACGTTTGTGGACACCAGTGTGAAAATACACTTGGAG
  GTTCTTGAAATTTGTGATAATGAAGAGGCCTTGGACACTGTGTCAATCATTAGTAACATC
  AGTCAGTCCTCCACACAAGTCAGATCTCCATCCCTACGTTACTCCAGGAAAGAAAACAGA
  TTTGTTTCATGTGACCTAGGGGAAACAGCCTCATACTCCCTCTTTTTGCCCACCAGTAAT
  CCTGATGGTGATATTAATATCTCCATTCCAGACACAGTAGAAGCACACAGGCAGAACAGT
  AAAAGGCAGCATCAAGAGAGGGATGGCTACCAGGAGGAAATCCAGTTGTTAAATAAAGCT
  TACAGAAAAAGAGAGGAAGAAAGCAAGGGTAGTTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001038705
• ORF Size: 2196 bp
• Insert Size: 5200
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. It has a 3kb 3' UTR sequence not found in the RefSeq sequence.

Reference Data

• RefSeq: NM_001038705.1, NP_001033794.1
• RefSeq Size: 2323
• RefSeq ORF: 2196
• Locus ID: 344758
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: This gene encodes a seven-transmembrane G protein coupled receptor (GPCR) class A family member. Although categorized as a class A GPCR, the encoded protein lacks the first two charged amino acids of the highly conserved Asp-Arg-Tyr (DRY) motif found in the third transmembrane helix of class A receptors which is important for efficient G protein-coupled signal transduction. Mice with a knockout of the orthologous gene are viable and have normal maturation of the ovarian follicle, but show enhanced fertility and ovulation. All GPCRs have a common structural architecture consisting of seven transmembrane alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptor, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits. The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. [provided by RefSeq, Jul 2017]