ACTN3 (NM_001104) Human Untagged Clone

CAT#: SC302972

ACTN3 (untagged)-Human actinin, alpha 3 (ACTN3)


  "NM_001104" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ACTN3
Synonyms ACTN3D
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001104 edited
GCCAGGAGCCCGATCGAGATGATGATGGTTATGCAGCCCGAGGGTCTGGGGGCCGGGGAG
GGGCGCTTTGCGGGCGGCGGCGGGGGCGGCGAGTACATGGAACAGGAGGAGGACTGGGAC
CGCGACCTGCTGCTGGACCCGGCCTGGGAGAAGCAGCAGCGGAAAACCTTCACTGCCTGG
TGCAACTCACACCTGCGCAAGGCAGGCACCCAGATCGAGAACATCGAGGAAGATTTCCGC
AATGGCCTCAAACTCATGCTGCTCCTGGAGGTCATTTCAGGTGAGAGGCTGCCTAGGCCA
GATAAAGGCAAGATGCGCTTCCACAAAATCGCCAACGTTAACAAGGCCCTGGACTTCATT
GCCAGCAAGGGGGTTAAACTGGTGTCCATTGGTGCTGAAGAGATTGTTGACGGGAACCTG
AAGATGACCCTGGGCATGATCTGGACCATCATCCTTCGCTTCGCCATCCAGGACATCTCT
GTGGAAGAAACCTCAGCCAAGGAAGGCTTGCTTCTGTGGTGCCAGAGGAAGACAGCACCG
TACCGCAACGTCAACGTGCAGAACTTCCACACCAGCTGGAAGGATGGCCTGGCCCTCTGT
GCCCTCATCCACCGACACCGCCCTGACCTCATCGACTACGCCAAACTGCGAAAGGATGAC
CCCATCGGAAACCTGAACACTGCCTTTGAGGTGGCAGAGAAATACCTGGACATCCCCAAG
ATGTTGGATGCAGAAGACATTGTGAACACCCCAAAGCCGGATGAGAAGGCCATCATGACC
TATGTGTCCTGCTTCTACCATGCCTTTGCCGGGGCTGAGCAGGCAGAGACAGCTGCCAAC
AGGATCTGCAAGGTGCTGGCAGTGAACCAGGAAAACGAGAAGCTGATGGAGGAGTATGAG
AAGCTTGCCAGTGAGCTGCTGGAGTGGATCCGCCGCACTGTCCCATGGCTGGAGAACCGT
GTGGGTGAGCCCAGCATGAGTGCCATGCAGCGCAAACTAGAGGACTTTCGGGACTACCGG
CGTCTGCACAAGCCGCCCCGCATTCAGGAAAAGTGCCAGCTGGAGATCAACTTCAACACA
CTGCAGACCAAGTTGCGGCTCAGCCACCGGCCTGCCTTCATGCCCTCCGAGGGCAAGCTG
GTCTCGGACATCGCCAACGCCTGGCGGGGGCTGGAGCAGGTGGAAAAGGGCTATGAGGAC
TGGCTGCTCTCGGAGATCCGGCGCCTGCAGCGACTCCAGCACCTGGCTGAGAAGTTCCGG
CAGAAGGCCTCCCTGCACGAAGCCTGGACCCGGGGAAAGGAGGAGATGCTGAGCCAGCGC
GACTACGATTCGGCTTTGCTACAGGAGGTGCGGGCGTTGCTGCGGCGCCACGAGGCCTTT
GAGAGCGACCTGGCGGCGCACCAGGACCGCGTGGAGCACATTGCCGCGCTGGCCCAGGAG
CTCAATGAGCTGGACTACCACGAGGCAGCCTCAGTGAATAGCCGCTGCCAGGCCATCTGC
GATCAGTGGGACAACCTGGGCACCCTGACCCAGAAGAGGCGGGATGCGCTAGAGCGGATG
GAGAAGCTCCTGGAGACCATTGACCAGCTGCAACTGGAGTTTGCCCGGCGGGCCGCGCCC
TTCAACAACTGGCTGGATGGTGCCGTGGAGGACCTGCAGGACGTGTGGCTGGTACACTCT
GTGGAGGAGACCCAGAGCCTGCTGACAGCGCACGATCAGTTCAAGGCAACACTGCCCGAG
GCTGACCGAGAGCGAGGTGCCATCATGGGCATCCAGGGTGAGATCCAGAAGATCTGCCAG
ACGTATGGGCTGCGGCCCTGCTCCACCAATCCCTACATCACCCTCAGCCCGCAGGACATC
AACACCAAGTGGGATATGGTCCGAAAGCTGGTGCCCAGCCGTGACCAGACACTGCAGGAG
GAGCTGGCACGGCAGCAGGTAAACGAGAGGCTCCGGCGACAGTTTGCGGCCCAGGCCAAT
GCCATTGGACCCTGGATCCAGGCGAAGGTGGAGGAAGTGGGGCGGCTGGCAGCAGGGCTA
GCTGGCTCTCTGGAGGAGCAGATGGCTGGGCTACGGCAGCAGGAGCAGAACATTATCAAC
TACAAGACTAACATTGACCGGCTGGAGGGTGACCACCAGCTGCTGCAGGAGAGCCTGGTG
TTCGACAATAAGCACACCGTCTACAGCATGGAGCACATCCGCGTGGGCTGGGAGCAGCTG
CTCACCTCCATTGCCCGCACCATCAATGAAGTGGAGAACCAGGTACTGACCCGAGACGCC
AAGGGACTGAGCCAGGAGCAGCTCAACGAGTTCCGAGCATCCTTCAACCACTTTGACAGG
AAGCGGAATGGGATGATGGAGCCTGATGACTTCCGAGCTTGCCTCATCTCCATGGGCTAT
GACCTGGGGGAAGTGGAGTTTGCTCGCATCATGACCATGGTGGACCCCAACGCAGCTGGG
GTGGTGACCTTCCAGGCCTTCATAGACTTCATGACCCGAGAGACAGCCGAGACTGACACG
ACTGAGCAAGTTG:TAGCTTCCTTCAAGATCTTGGCAGGAGACAAGAACTACATCACCCC
CGAGGAGCTGCGGCGCGAGCTCCCTGCCAAGCAGGCCGAGTACTGCATCCGCCGTATGGT
GCCCTACAAGGGATCCGGGGCCCCGGCTGGAGCCCTGGACTACGTGGCCTTCTCCAGTGC
CCTCTATGGGGAGAGCGACCTTTGACCCCAACCACTGAGGTTCTCTATGCAAGATGGAGA
GAGGATGCACCCTGTGGCTGATCCCATCCGTCCCTCGGAGCAAGGGCCTAAGAGAAAAGC
CAGCCAAGTGCTTCT
Restriction Sites Please inquire     
ACCN NM_001104
Insert Size 2800 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001104.1.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001104.1, NP_001095.1
RefSeq Size 2858 bp
RefSeq ORF 2706 bp
Locus ID 89
Cytogenetics 11q13.2
Protein Pathways Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction
Gene Summary 'This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014]'
Transcript Variant: This variant (1, coding) represents the protein-coding allele and encodes isoform 1.

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