BNIP1 (NM_001205) Human Untagged Clone

CAT#: SC302991

BNIP1 (untagged)-Human BCL2/adenovirus E1B 19kDa interacting protein 1 (BNIP1), transcript variant BNIP1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BNIP1
• Synonyms: NIP1; SEC20; TRG-8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001205 edited
  ATGGCGGCTCCCCAAGACGTCCACGTCCGGATCTGTAACCAAGAGATTGTCAAATTTGAC
  CTGGAGGTGAAGGCGCTTATTCAGGATATCCGTGATTGTTCAGGACCCTTAAGTGCTCTT
  ACTGAACTGAATACTAAAGTAAAAGCGAAATTTCAACAGTTGCGTCACAGAATACAGGAC
  CTGGAGCAGTTGGCTAAAGAGCAAGACAAAGAATCAGAGAAACAACTTCTACTCCAGGAA
  GTGGAGAATCACAAAAAGCAGATGCTCAGCAATCAGGCCTCATGGAGGAAAGCTAATCTC
  ACCTGCAAAATTGCAATCGACAATCTAGAGAAAGCAGAACTTCTTCAGGGAGGAGATCTC
  TTAAGGCAAAGGAAAACCACCAAAGAGAGCCTGGCCCAGACATCCAGTACCATCACTGAG
  AGCCTCATGGGGATCAGCAGGATGATGGCCCAGCAGGTCCAGCAGAGCGAGGAGGCCATG
  CAGTCTCTAGTCACTTCTTCACGAACGATCCTGGATGCAAATGAAGAATTTAAGTCCATG
  TCGGGCACCATCCAGCTGGGCCGGAAGCTTATCACAAAATACAATCGCCGGGAGCTGACG
  GACAAGCTTCTCATCTTCCTTGCGCTAGCCCTGTTTCTTGCTACGGTCCTCTATATTGTG
  AAAAAGCGGCTCTTTCCATTTTTGTGAGATCCCAAAGGTGCCAGTTCTGGCCCTTTCAGC
  TCCTGTTTCAGGATCTGTCCTGGTTCCTGAGCTCTAGGCTGCTAAGCTGAGCCACACACC
  CCTCCGTTTTGCACCAGTTGCCTGCAGGTTGGATGGAACACAGTGCCCCACTTTTCTGCA
  AGTAGCTGGCTTGTAAAGGGTGAACAGAGCCATGGGAGGAAGGTCTGGCATTGGGATGCC
  GCCCTGGGGACATACGAACCGCCTCCTTCCACCATTGTGCACTATGGGAGGCCGCTGCTG
  CGTGGAGCACTTAAAGTCCAGCCTCCAGGACCGGATGCCCCTCCTGTCTCCCGCTCCCAT
  CGTGCCCTTAAATGCCAGATCTGGTGGAGGGAAGAGAGAAGAGGTAGGAAGAAAGGTGAT
  GAAAACTCCTGGTCATTCCTTGAATAAACTTGATTTTTTTTAATAGAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001205 unedited
  CGGCACGNAGGATGNNGCGGCTCCCCAAAACGTCCACGTCCGGCTCTGTAACCAAAAGAT
  TGTCAAATTTGACCTGGAGGTGAAGGCGCTTATTCAGGATATCCGTGATTGTTCAGGACC
  CTTAAGTGCTCTTACTGAACTGAATACTAAAGTAAAAGCGAAATTTCAACAGTTGCGTCA
  CAGAATACAGGACCTGGAGCAGTTGGCTAAAGAGCAAGACAAAGAATCAGAGAAACAACT
  TCTACTCCAGGAAGTGGAGAATCACAAAAAGCAGATGCTCAGCAATCAGGCCTCATGGAG
  GAAAGCTAATCTCACCTGCAAAATTGCAATCGACAATCTAGAGAAAGCAGAACTTCTTCA
  GGGAGGAGATCTCTTAAGGCAAAGGAAAACCACCAAAGAGAGCCTGGCCCAGACATCCAG
  TACCATCACTGAGAGCCTCATGGGGATCAGCAGGATGATGGCCCAGCAGGTCCAGCAGAG
  CGAGGAGGCCATGCAGTCTCTAGTCACTTCTTCACGAACGATCCTGGATGCAAATGAAGA
  ATTTAAGTCCATGTCGGGCACCATCCAGCTGGGCCGGAAGCTTATCACAAAATACAATCG
  CCGGGAGCTGACGGACAAGCTTCTCATCTTCCTTGCGCTAGCCCTGTTTCTTGCTACGGT
  CCTCTATATTGTGAAAAAGCGGCTCTTTCCATTTTTGTGAGATCCCAAAGGTGCCAGTTC
  TGGCCCTTTCAGCTCCTGTTTCAGGATCTGTCCTGGTTCCTGAGCTCTAGGCTGCTAAGC
  TGAGCCACACACCCCTCCGTTTGCACCAGTTGNCCTGCAGTTGGATGGAACACAGTGCCC
  CACTTTNTCTGCAGTAGCT
  
• Restriction Sites: Please inquire
• ACCN: NM_001205
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205.1, NP_001196.1
• RefSeq Size: 1100 bp
• RefSeq ORF: 687 bp
• Locus ID: 662
• Cytogenetics: 5q35.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini. [provided by RefSeq, Mar 2011]'
  Transcript Variant: Transcript variant BNIP1 contains the entire coding region of the gene. This variant contains a fully conserved BH3 domain, which has been associated with pro-apoptotic function.