CD30 (TNFRSF8) (NM_001243) Human Untagged Clone

CAT#: SC302996

TNFRSF8 (untagged)-Human tumor necrosis factor receptor superfamily, member 8 (TNFRSF8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TNFRSF8
• Synonyms: CD30; D1S166E; Ki-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001243 edited
  GAGTGTGCTGGAGCCTGAAGTCCACGCGCGCGGCTGAGAACCGCCGGGACCGCACGTGGG
  CGCCGCGCGCTTCCCCCGCTTCCCAGGTGGGCGCCGGCCGCCAGGCCACCTCACGTCCGG
  CCCCGGGGATGCGCGTCCTCCTCGCCGCGCTGGGACTGCTGTTCCTGGGGGCGCTACGAG
  CCTTCCCACAGGATCGACCCTTCGAGGACACCTGTCATGGAAACCCCAGCCACTACTATG
  ACAAGGCTGTCAGGAGGTGCTGTTACCGCTGCCCCATGGGGCTGTTCCCGACACAGCAGT
  GCCCACAGAGGCCTACTGACTGCAGGAAGCAGTGTGAGCCTGACTACTACCTGGATGAGG
  CCGACCGCTGTACAGCCTGCGTGACTTGTTCTCGAGACGACCTCGTGGAGAAGACGCCGT
  GTGCATGGAACTCCTCCCGTGTCTGCGAATGTCGACCCGGCATGTTCTGTTCCACGTCTG
  CCGTCAACTCCTGTGCCCGCTGCTTCTTCCATTCTGTCTGTCCGGCAGGGATGATTGTCA
  AGTTCCCAGGCACGGCGCAGAAGAACACGGTCTGTGAGCCGGCTTCCCCAGGGGTCAGCC
  CTGCCTGTGCCAGCCCAGAGAACTGCAAGGAACCCTCCAGTGGCACCATCCCCCAGGCCA
  AGCCCACCCCGGTGTCCCCAGCAACCTCCAGTGCCAGCACCATGCCTGTAAGAGGGGGCA
  CCCGCCTCGCCCAGGAAGCTGCTTCTAAACTGACGAGGGCTCCCGACTCTCCCTCCTCTG
  TGGGAAGGCCTAGTTCAGATCCAGGTCTGTCCCCAACACAGCCATGCCCAGAGGGGTCTG
  GTGATTGCAGAAAGCAGTGTGAGCCCGACTACTACCTGGACGAGGCCGGCCGCTGCACGG
  CCTGCGTGAGCTGTTCTCGAGATGACCTTGTGGAGAAGACGCCATGTGCATGGAACTCCT
  CCCGCACCTGCGAATGTCGACCTGGCATGATCTGTGCCACATCAGCCACCAACTCCTGTG
  CCCGCTGTGTCCCCTACCCAATCTGTGCAGCAGAGACGGTCACCAAGCCCCAGGATATGG
  CTGAGAAGGACACCACCTTTGAGGCGCCACCCCTGGGGACCCAGCCGGACTGCAACCCCA
  CCCCAGAGAATGGCGAGGCGCCTGCCAGCACCAGCCCCACTCAGAGCTTGCTGGTGGACT
  CCCAGGCCAGTAAGACGCTGCCCATCCCAACCAGCGCTCCCGTCGCTCTCTCCTCCACGG
  GGAAGCCCGTTCTGGATGCAGGGCCAGTGCTCTTCTGGGTGATCCTGGTGTTGGTTGTGG
  TGGTCGGCTCCAGCGCCTTCCTCCTGTGCCACCGGAGGGCCTGCAGGAAGCGAATTCGGC
  AGAAGCTCCACCTGTGCTACCCGGTCCAGACCTCCCAGCCCAAGCTAGAGCTTGTGGATT
  CCAGACCCAGGAGGAGCTCAACGCAGCTGAGGAGTGGTGCGTCGGTGACAGAACCCGTCG
  CGGAAGAGCGAGGGTTAATGAGCCAGCCACTGATGGAGACCTGCCACAGCGTGGGGGCAG
  CCTACCTGGAGAGCCTGCCGCTGCAGGATGCCAGCCCGGCCGGGGGCCCCTCGTCCCCCA
  GGGACCTTCCTGAGCCCCGGGTGTCCACGGAGCACACCAATAACAAGATTGAGAAAATCT
  ACATCATGAAGGCTGACACCGTGATCGTGGGGACCGTGAAGGCTGAGCTGCCGGAGGGCC
  GGGGCCTGGCGGGGCCAGCAGAGCCCGAGTTGGAGGAGGAGCTGGAGGCGGACCATACCC
  CCCACTACCCCGAGCAGGAGACAGAACCGCCTCTGGGCAGCTGCAGCGATGTCATGCTCT
  CAGTGGAAGAGGAAGGGAAAGAAGACCCCTTGCCCACAGCTGCCTCTGGAAAGTGAGGCC
  TGGGCTGGGCTGGGGCTAGGAGGGCAGCAGGGTGGCCTCTGGGAGGCCAGGATGGCACTG
  TTGGCACCGAGGTTGGGGGCAGAGGCCCATCTGGCCTGAACTGAGGCTCCAGCATCTAGT
  GGTGGACCGGCCGGTCACTGCAGGGGTATGGTGGTCTCTGCTTGCAT
  
  5' Read Nucleotide Sequence

  >Reverse primer walk for NM_001243 unedited
  AAAGTCGGGNAGCCCTCGTCAGTTTAGAAGCAGCTTCCTGGGCGAGGCGGGTGCCCCCTC
  TTACAGGCATGGTGCTGGCACTGGAGGTTGCTGGGGACACCGGGGTGGGCTTGGCCTGGG
  GGATGGTGCCACTGGAGGGTTCCTTGCAGTTCTCTGGGCTGGCACAGGCAGGGCTGACCC
  CTGGGGAAGCCGGCTCACAGACCGTGTTCTTCTGCGCCGTGCCTGGGAACTTGACAATCA
  TCCCTGCCGGACAGACAGAATGGAAGAAGCAGCGGGCACAGGAGTTGACGGCAGACGTGG
  AACAGAACATGCCGGGTCGACATTCGCAGACACGGGAGGAGTTCCATGCACACGGCGTCT
  TCTCCACGAGGTCGTCTCGAGAACAAGTCACGCAGGCTGTACAGCGGTCGGCCTCATCCA
  GGTAGTAGTCAGGCTCACACTGCTTCCTGCAGTCAGTAGGCCTCTGTGGGCACTGCTGTG
  TCGGGAACAGCCCCATGGGGCAGCGGTAACAGCACCTCCTGACAGCCTTGTCATAGTAGT
  GGCTGGGGTTTCCATGACAGGTGTCCTCGAAGGGTCGATCCTGTGGGAAGGCTCGTAGCG
  CCCCCAGGAACAGCAGTCCCAGCGCGGCGAGGAGGACGCGCATCCCCGGNGCCGGACGTG
  AGGTGGCCTGGCGGCCGGCGCCCACCTGNGAAGCGGNGGAAGCGCGNCGCGCCCACGTGC
  GGTCCCGGGCGTTCTCAGCCGCGCGCGTGGACTTTCAGCTCCAGCACACTCAAGGGCGAA
  TTCTGCAGATATCGTACCAGATCTGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAN
  AATTTCTGACGGTCACTAAACGAGCTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001243 unedited
  CATTGGNGAATGGCACTTNCCAGGNCCAGAAAAGCACTGGGGNAGGGTCACAGGGATGCC
  ACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTT
  GGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTATGCA
  AGCAGAGACCACCATACCCCTGCAGTGACCGGCCGGTCCACCACTAGATGCTGGAGCCTC
  AGTTCAGGCCAGATGGGCCTCTGCCCCCAACCTCGGTGCCAACAGTGCCATCCTGGCCTC
  CCAGAGGCCACCCTGCTGCCCTCCTAGCCCCAGCCCAGCCCAGGCCTCACTTTCCAGAGG
  CAGCTGTGGGCAAGGGGTCTTCTTTCCCTTCCTCTTCCACTGAGAGCATGACATCGCTGC
  AGCTGCCCAGAGGCGGTTCTGTCTCCTGCTCGGGGTAGTGGGGGGTATGGTCCGCCTCCA
  GCTCCTCCTCCAACTCGGGCTCTGCTGGCCCCGCCAGGCCCCGGCCCTCCGGCAGCTCAG
  CCTTCACGGTCCCCACGATCACGGTGTCAGCCTTCATGATGTAGATTTTCTCAATCTTGT
  TATTGGTGTGCTCCGTGGACACCCGGNGCTCAAGAAAGTCCCTGGGGGACGAGGGGCCCC
  CGGCCGGGCTGGCATCCTGCAGCGGCAGGCTCTCCAGGTAGGCTGCCCCCACGCTGTGGC
  AAGTCTCCATCAGTGGCTGGCTCATTAACCCTCGCTCTTCCGCGACGGGTTCTGTCACCG
  ACGCACCACTCCTTAGCTGCGTTTGAGCTCCTNCCTGGGTCGGGAATCCACAAGCTCCTA
  GCTGGGGCTGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_001243
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acids.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243.3, NP_001234.2
• RefSeq Size: 3686 bp
• RefSeq ORF: 1788 bp
• Locus ID: 943
• Cytogenetics: 1p36.22
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction
• Gene Summary: 'The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).