CD30 (TNFRSF8) (NM_001243) Human Untagged Clone

CAT#: SC302996

TNFRSF8 (untagged)-Human tumor necrosis factor receptor superfamily, member 8 (TNFRSF8), transcript variant 1


  "NM_001243" in other vectors (6)

Reconstitution Protocol

USD 1,010.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TNFRSF8
Synonyms CD30; D1S166E; Ki-1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001243 edited
GAGTGTGCTGGAGCCTGAAGTCCACGCGCGCGGCTGAGAACCGCCGGGACCGCACGTGGG
CGCCGCGCGCTTCCCCCGCTTCCCAGGTGGGCGCCGGCCGCCAGGCCACCTCACGTCCGG
CCCCGGGGATGCGCGTCCTCCTCGCCGCGCTGGGACTGCTGTTCCTGGGGGCGCTACGAG
CCTTCCCACAGGATCGACCCTTCGAGGACACCTGTCATGGAAACCCCAGCCACTACTATG
ACAAGGCTGTCAGGAGGTGCTGTTACCGCTGCCCCATGGGGCTGTTCCCGACACAGCAGT
GCCCACAGAGGCCTACTGACTGCAGGAAGCAGTGTGAGCCTGACTACTACCTGGATGAGG
CCGACCGCTGTACAGCCTGCGTGACTTGTTCTCGAGACGACCTCGTGGAGAAGACGCCGT
GTGCATGGAACTCCTCCCGTGTCTGCGAATGTCGACCCGGCATGTTCTGTTCCACGTCTG
CCGTCAACTCCTGTGCCCGCTGCTTCTTCCATTCTGTCTGTCCGGCAGGGATGATTGTCA
AGTTCCCAGGCACGGCGCAGAAGAACACGGTCTGTGAGCCGGCTTCCCCAGGGGTCAGCC
CTGCCTGTGCCAGCCCAGAGAACTGCAAGGAACCCTCCAGTGGCACCATCCCCCAGGCCA
AGCCCACCCCGGTGTCCCCAGCAACCTCCAGTGCCAGCACCATGCCTGTAAGAGGGGGCA
CCCGCCTCGCCCAGGAAGCTGCTTCTAAACTGACGAGGGCTCCCGACTCTCCCTCCTCTG
TGGGAAGGCCTAGTTCAGATCCAGGTCTGTCCCCAACACAGCCATGCCCAGAGGGGTCTG
GTGATTGCAGAAAGCAGTGTGAGCCCGACTACTACCTGGACGAGGCCGGCCGCTGCACGG
CCTGCGTGAGCTGTTCTCGAGATGACCTTGTGGAGAAGACGCCATGTGCATGGAACTCCT
CCCGCACCTGCGAATGTCGACCTGGCATGATCTGTGCCACATCAGCCACCAACTCCTGTG
CCCGCTGTGTCCCCTACCCAATCTGTGCAGCAGAGACGGTCACCAAGCCCCAGGATATGG
CTGAGAAGGACACCACCTTTGAGGCGCCACCCCTGGGGACCCAGCCGGACTGCAACCCCA
CCCCAGAGAATGGCGAGGCGCCTGCCAGCACCAGCCCCACTCAGAGCTTGCTGGTGGACT
CCCAGGCCAGTAAGACGCTGCCCATCCCAACCAGCGCTCCCGTCGCTCTCTCCTCCACGG
GGAAGCCCGTTCTGGATGCAGGGCCAGTGCTCTTCTGGGTGATCCTGGTGTTGGTTGTGG
TGGTCGGCTCCAGCGCCTTCCTCCTGTGCCACCGGAGGGCCTGCAGGAAGCGAATTCGGC
AGAAGCTCCACCTGTGCTACCCGGTCCAGACCTCCCAGCCCAAGCTAGAGCTTGTGGATT
CCAGACCCAGGAGGAGCTCAACGCAGCTGAGGAGTGGTGCGTCGGTGACAGAACCCGTCG
CGGAAGAGCGAGGGTTAATGAGCCAGCCACTGATGGAGACCTGCCACAGCGTGGGGGCAG
CCTACCTGGAGAGCCTGCCGCTGCAGGATGCCAGCCCGGCCGGGGGCCCCTCGTCCCCCA
GGGACCTTCCTGAGCCCCGGGTGTCCACGGAGCACACCAATAACAAGATTGAGAAAATCT
ACATCATGAAGGCTGACACCGTGATCGTGGGGACCGTGAAGGCTGAGCTGCCGGAGGGCC
GGGGCCTGGCGGGGCCAGCAGAGCCCGAGTTGGAGGAGGAGCTGGAGGCGGACCATACCC
CCCACTACCCCGAGCAGGAGACAGAACCGCCTCTGGGCAGCTGCAGCGATGTCATGCTCT
CAGTGGAAGAGGAAGGGAAAGAAGACCCCTTGCCCACAGCTGCCTCTGGAAAGTGAGGCC
TGGGCTGGGCTGGGGCTAGGAGGGCAGCAGGGTGGCCTCTGGGAGGCCAGGATGGCACTG
TTGGCACCGAGGTTGGGGGCAGAGGCCCATCTGGCCTGAACTGAGGCTCCAGCATCTAGT
GGTGGACCGGCCGGTCACTGCAGGGGTATGGTGGTCTCTGCTTGCAT
>Reverse primer walk for NM_001243 unedited
AAAGTCGGGNAGCCCTCGTCAGTTTAGAAGCAGCTTCCTGGGCGAGGCGGGTGCCCCCTC
TTACAGGCATGGTGCTGGCACTGGAGGTTGCTGGGGACACCGGGGTGGGCTTGGCCTGGG
GGATGGTGCCACTGGAGGGTTCCTTGCAGTTCTCTGGGCTGGCACAGGCAGGGCTGACCC
CTGGGGAAGCCGGCTCACAGACCGTGTTCTTCTGCGCCGTGCCTGGGAACTTGACAATCA
TCCCTGCCGGACAGACAGAATGGAAGAAGCAGCGGGCACAGGAGTTGACGGCAGACGTGG
AACAGAACATGCCGGGTCGACATTCGCAGACACGGGAGGAGTTCCATGCACACGGCGTCT
TCTCCACGAGGTCGTCTCGAGAACAAGTCACGCAGGCTGTACAGCGGTCGGCCTCATCCA
GGTAGTAGTCAGGCTCACACTGCTTCCTGCAGTCAGTAGGCCTCTGTGGGCACTGCTGTG
TCGGGAACAGCCCCATGGGGCAGCGGTAACAGCACCTCCTGACAGCCTTGTCATAGTAGT
GGCTGGGGTTTCCATGACAGGTGTCCTCGAAGGGTCGATCCTGTGGGAAGGCTCGTAGCG
CCCCCAGGAACAGCAGTCCCAGCGCGGCGAGGAGGACGCGCATCCCCGGNGCCGGACGTG
AGGTGGCCTGGCGGCCGGCGCCCACCTGNGAAGCGGNGGAAGCGCGNCGCGCCCACGTGC
GGTCCCGGGCGTTCTCAGCCGCGCGCGTGGACTTTCAGCTCCAGCACACTCAAGGGCGAA
TTCTGCAGATATCGTACCAGATCTGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAN
AATTTCTGACGGTCACTAAACGAGCTCT
>OriGene 3' read for NM_001243 unedited
CATTGGNGAATGGCACTTNCCAGGNCCAGAAAAGCACTGGGGNAGGGTCACAGGGATGCC
ACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTT
GGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTATGCA
AGCAGAGACCACCATACCCCTGCAGTGACCGGCCGGTCCACCACTAGATGCTGGAGCCTC
AGTTCAGGCCAGATGGGCCTCTGCCCCCAACCTCGGTGCCAACAGTGCCATCCTGGCCTC
CCAGAGGCCACCCTGCTGCCCTCCTAGCCCCAGCCCAGCCCAGGCCTCACTTTCCAGAGG
CAGCTGTGGGCAAGGGGTCTTCTTTCCCTTCCTCTTCCACTGAGAGCATGACATCGCTGC
AGCTGCCCAGAGGCGGTTCTGTCTCCTGCTCGGGGTAGTGGGGGGTATGGTCCGCCTCCA
GCTCCTCCTCCAACTCGGGCTCTGCTGGCCCCGCCAGGCCCCGGCCCTCCGGCAGCTCAG
CCTTCACGGTCCCCACGATCACGGTGTCAGCCTTCATGATGTAGATTTTCTCAATCTTGT
TATTGGTGTGCTCCGTGGACACCCGGNGCTCAAGAAAGTCCCTGGGGGACGAGGGGCCCC
CGGCCGGGCTGGCATCCTGCAGCGGCAGGCTCTCCAGGTAGGCTGCCCCCACGCTGTGGC
AAGTCTCCATCAGTGGCTGGCTCATTAACCCTCGCTCTTCCGCGACGGGTTCTGTCACCG
ACGCACCACTCCTTAGCTGCGTTTGAGCTCCTNCCTGGGTCGGGAATCCACAAGCTCCTA
GCTGGGGCTGGG
Restriction Sites Please inquire     
ACCN NM_001243
Insert Size 2100 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acids.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001243.3, NP_001234.2
RefSeq Size 3686 bp
RefSeq ORF 1788 bp
Locus ID 943
Cytogenetics 1p36.22
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction
Gene Summary 'The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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