Collagen II (COL2A1) (NM_001844) Human Untagged Clone
CAT#: SC303078
COL2A1 (untagged)-Human collagen, type II, alpha 1 (COL2A1), transcript variant 1
"NM_001844" in other vectors (6)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | COL2A1 |
Synonyms | ANFH; AOM; COL11A3; SEDC; STL1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>NCBI ORF sequence for NM_001844, the custom clone sequence may differ by one or more nucleotides
ATGATTCGCCTCGGGGCTCCCCAGACGCTGGTGCTGCTGACGCTGCTCGTCGCCGCTGTCCTTCGGTGTC AGGGCCAGGATGTCCAGGAGGCTGGCAGCTGTGTGCAGGATGGGCAGAGGTATAATGATAAGGATGTGTG GAAGCCGGAGCCCTGCCGGATCTGTGTCTGTGACACTGGGACTGTCCTCTGCGACGACATAATCTGTGAA GACGTGAAAGACTGCCTCAGCCCTGAGATCCCCTTCGGAGAGTGCTGCCCCATCTGCCCAACTGACCTCG CCACTGCCAGTGGGCAACCAGGACCAAAGGGACAGAAAGGAGAACCTGGAGACATCAAGGATATTGTAGG ACCCAAAGGACCTCCTGGGCCTCAGGGACCTGCAGGGGAACAAGGACCCAGAGGGGATCGTGGTGACAAA GGTGAAAAAGGTGCCCCTGGACCTCGTGGCAGAGATGGAGAACCTGGGACCCCTGGAAATCCTGGCCCCC CTGGTCCTCCCGGCCCCCCTGGTCCCCCTGGTCTTGGTGGAAACTTTGCTGCCCAGATGGCTGGAGGATT TGATGAAAAGGCTGGTGGCGCCCAGTTGGGAGTAATGCAAGGACCAATGGGCCCCATGGGACCTCGAGGA CCTCCAGGCCCTGCAGGTGCTCCTGGGCCTCAAGGATTTCAAGGCAATCCTGGTGAACCTGGTGAACCTG GTGTCTCTGGTCCCATGGGTCCCCGTGGTCCTCCTGGTCCCCCTGGAAAGCCTGGTGATGATGGTGAAGC TGGAAAACCTGGAAAAGCTGGTGAAAGGGGTCCGCCTGGTCCTCAGGGTGCTCGTGGTTTCCCAGGAACC CCAGGCCTTCCTGGTGTCAAAGGTCACAGAGGTTATCCAGGCCTGGACGGTGCTAAGGGAGAGGCGGGTG CTCCTGGTGTGAAGGGTGAGAGTGGTTCCCCGGGTGAGAACGGATCTCCGGGCCCAATGGGTCCTCGTGG CCTGCCTGGTGAAAGAGGACGGACTGGCCCTGCTGGCGCTGCGGGTGCCCGAGGCAACGATGGTCAGCCA GGCCCCGCAGGGCCTCCGGGTCCTGTCGGTCCTGCTGGTGGTCCTGGCTTCCCTGGTGCTCCTGGAGCCA AGGGTGAAGCCGGCCCCACTGGTGCCCGTGGTCCTGAAGGTGCTCAAGGTCCTCGCGGTGAACCTGGTAC TCCTGGGTCCCCTGGGCCTGCTGGTGCCTCCGGTAACCCTGGAACAGATGGAATTCCTGGAGCCAAAGGA TCTGCTGGTGCTCCTGGCATTGCTGGTGCTCCTGGCTTCCCTGGGCCACGGGGCCCTCCTGGCCCTCAAG GTGCAACTGGTCCTCTGGGCCCGAAAGGTCAGACGGGTGAACCTGGTATTGCTGGCTTCAAAGGTGAACA AGGCCCCAAGGGAGAACCTGGCCCTGCTGGCCCCCAGGGAGCCCCTGGACCCGCTGGTGAAGAAGGCAAG AGAGGTGCCCGTGGAGAGCCTGGTGGCGTTGGGCCCATCGGTCCCCCTGGAGAAAGAGGTGCTCCCGGCA ACCGCGGTTTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGAGCCCCTGGAGAGCGAGGGCCCAGTGG TCTTGCTGGCCCCAAGGGAGCCAACGGTGACCCTGGCCGTCCTGGAGAACCTGGCCTTCCTGGAGCCCGG GGTCTCACTGGCCGCCCTGGTGATGCTGGTCCTCAAGGCAAAGTTGGCCCTTCTGGAGCCCCTGGTGAAG ATGGTCGTCCTGGACCTCCAGGTCCTCAGGGGGCTCGTGGGCAGCCTGGTGTCATGGGTTTCCCTGGCCC CAAAGGTGCCAACGGTGAGCCTGGCAAAGCTGGTGAGAAGGGACTGCCTGGTGCTCCTGGTCTGAGGGGT CTTCCTGGCAAAGATGGTGAGACAGGTGCTGCAGGACCCCCTGGCCCTGCTGGACCTGCTGGTGAACGAG GCGAGCAGGGTGCTCCTGGGCCATCTGGGTTCCAGGGACTTCCTGGCCCTCCTGGTCCCCCAGGTGAAGG TGGAAAACCAGGTGACCAGGGTGTTCCCGGTGAAGCTGGAGCCCCTGGCCTCGTGGGTCCCAGGGGTGAA CGAGGTTTCCCAGGTGAACGTGGCTCTCCCGGTGCCCAGGGCCTCCAGGGTCCCCGTGGCCTCCCCGGCA CTCCTGGCACTGATGGTCCCAAAGGTGCATCTGGCCCAGCAGGCCCCCCTGGGGCTCAGGGCCCTCCAGG TCTTCAGGGAATGCCTGGCGAGAGGGGAGCAGCTGGTATCGCTGGGCCCAAAGGCGACAGGGGTGACGTT GGTGAGAAAGGCCCTGAGGGAGCCCCTGGAAAGGATGGTGGACGAGGCCTGACAGGTCCCATTGGCCCCC CTGGCCCAGCTGGTGCTAATGGCGAGAAGGGAGAAGTTGGACCTCCTGGTCCTGCAGGAAGTGCTGGTGC TCGTGGCGCTCCGGGTGAACGTGGAGAGACTGGGCCCCCCGGACCAGCGGGATTTGCTGGGCCTCCTGGT GCTGATGGCCAGCCTGGGGCCAAGGGTGAGCAAGGAGAGGCCGGCCAGAAAGGCGATGCTGGTGCCCCTG GTCCTCAGGGCCCCTCTGGAGCACCTGGGCCTCAGGGTCCTACTGGAGTGACTGGTCCTAAAGGAGCCCG AGGTGCCCAAGGCCCCCCGGGAGCCACTGGATTCCCTGGAGCTGCTGGCCGCGTTGGACCCCCAGGCTCC AATGGCAACCCTGGACCCCCTGGTCCCCCTGGTCCTTCTGGAAAAGATGGTCCCAAAGGTGCTCGAGGAG ACAGCGGCCCCCCTGGCCGAGCTGGTGAACCCGGCCTCCAAGGTCCTGCTGGACCCCCTGGCGAGAAGGG AGAGCCTGGAGATGACGGTCCCTCTGGTGCCGAAGGTCCACCAGGTCCCCAGGGTCTGGCTGGTCAGAGA GGCATCGTCGGTCTGCCTGGGCAACGTGGTGAGAGAGGATTCCCTGGCTTGCCTGGCCCGTCGGGTGAGC CCGGCAAGCAGGGTGCTCCTGGAGCATCTGGAGACAGAGGTCCTCCTGGCCCCGTGGGTCCTCCTGGCCT GACGGGTCCTGCAGGTGAACCTGGACGAGAGGGAAGCCCCGGTGCTGATGGCCCCCCTGGCAGAGATGGC GCTGCTGGAGTCAAGGGTGATCGTGGTGAGACTGGTGCTGTGGGAGCTCCTGGAGCCCCTGGGCCCCCTG GCTCCCCTGGCCCCGCTGGTCCAACTGGCAAGCAAGGAGACAGAGGAGAAGCTGGTGCACAAGGCCCCAT GGGACCCTCAGGACCAGCTGGAGCCCGGGGAATCCAGGGTCCTCAAGGCCCCAGAGGTGACAAAGGAGAG GCTGGAGAGCCTGGCGAGAGAGGCCTGAAGGGACACCGTGGCTTCACTGGTCTGCAGGGTCTGCCCGGCC CTCCTGGTCCTTCTGGAGACCAAGGTGCTTCTGGTCCTGCTGGTCCTTCTGGCCCTAGAGGTCCTCCTGG CCCCGTCGGTCCCTCTGGCAAAGATGGTGCTAATGGAATCCCTGGCCCCATTGGGCCTCCTGGTCCCCGT GGACGATCAGGCGAAACCGGCCCTGCTGGTCCTCCTGGAAATCCTGGACCCCCTGGTCCTCCAGGTCCCC CTGGCCCTGGCATCGACATGTCCGCCTTTGCTGGCTTAGGCCCGAGAGAGAAGGGCCCCGACCCCCTGCA GTACATGCGGGCCGACCAGGCAGCCGGTGGCCTGAGACAGCATGACGCCGAGGTGGATGCCACACTCAAG TCCCTCAACAACCAGATTGAGAGCATCCGCAGCCCCGAGGGCTCCCGCAAGAACCCTGCTCGCACCTGCA GAGACCTGAAACTCTGCCACCCTGAGTGGAAGAGTGGAGACTACTGGATTGACCCCAACCAAGGCTGCAC CTTGGACGCCATGAAGGTTTTCTGCAACATGGAGACTGGCGAGACTTGCGTCTACCCCAATCCAGCAAAC GTTCCCAAGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAACACATCTGGTTTGGAGAAACCATCA ATGGTGGCTTCCATTTCAGCTATGGAGATGACAATCTGGCTCCCAACACTGCCAACGTCCAGATGACCTT CCTACGCCTGCTGTCCACGGAAGGCTCCCAGAACATCACCTACCACTGCAAGAACAGCATTGCCTATCTG GACGAAGCAGCTGGCAACCTCAAGAAGGCCCTGCTCATCCAGGGCTCCAATGACGTGGAGATCCGGGCAG AGGGCAATAGCAGGTTCACGTACACTGCCCTGAAGGATGGCTGCACGAAACATACCGGTAAGTGGGGCAA GACTGTTATCGAGTACCGGTCACAGAAGACCTCACGCCTCCCCATCATTGACATTGCACCCATGGACATA GGAGGGCCCGAGCAGGAATTCGGTGTGGACATAGGGCCGGTCTGCTTCTTGTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001844 |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001844.4, NP_001835.3 |
RefSeq Size | 5087 bp |
RefSeq ORF | 4464 bp |
Locus ID | 1280 |
Cytogenetics | 12q13.11 |
Protein Pathways | ECM-receptor interaction, Focal adhesion |
Gene Summary | 'This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]' Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218041 | COL2A1 (Myc-DDK-tagged)-Human collagen, type II, alpha 1 (COL2A1), transcript variant 1 |
USD 1,560.00 |
|
RG218041 | COL2A1 (GFP-tagged) - Human collagen, type II, alpha 1 (COL2A1), transcript variant 1 |
USD 1,720.00 |
|
RC218041L1 | Lenti-ORF clone of COL2A1 (Myc-DDK-tagged)-Human collagen, type II, alpha 1 (COL2A1), transcript variant 1 |
USD 1,760.00 |
|
RC218041L2 | Lenti-ORF clone of COL2A1 (mGFP-tagged)-Human collagen, type II, alpha 1 (COL2A1), transcript variant 1 |
USD 1,760.00 |
|
RC218041L3 | Lenti-ORF clone of COL2A1 (Myc-DDK-tagged)-Human collagen, type II, alpha 1 (COL2A1), transcript variant 1 |
USD 1,760.00 |
|
RC218041L4 | Lenti-ORF clone of COL2A1 (mGFP-tagged)-Human collagen, type II, alpha 1 (COL2A1), transcript variant 1 |
USD 1,760.00 |
{0} Product Review(s)
Be the first one to submit a review