Collagen IX (COL9A1) (NM_001851) Human Untagged Clone

CAT#: SC303083

COL9A1 (untagged)-Human collagen, type IX, alpha 1 (COL9A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL9A1
• Synonyms: DJ149L1.1.2; EDM6; MED; STL4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001851, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGACCTGCTGGAAAATTCCAGTTTTCTTCTTTGTGTGCAGTTTCCTGGAACCCTGGGCATCTGCAG
  CTGTCAAGCGTCGCCCCAGATTCCCTGTCAATTCCAATTCTAATGGTGGAAATGAACTCTGTCCAAAGAT
  CAGGATTGGCCAAGATGACTTACCAGGGTTTGATCTGATCTCTCAGTTCCAGGTAGATAAAGCAGCATCT
  AGAAGAGCTATCCAGAGAGTAGTGGGATCAGCTACATTGCAGGTGGCTTACAAGTTGGGAAATAATGTAG
  ACTTCAGGATTCCAACTAGGAATTTATATCCCAGTGGACTGCCTGAAGAATACTCCTTCTTGACGACGTT
  TCGAATGACTGGAAGCACTCTCAAAAAGAACTGGAACATTTGGCAGATTCAGGATTCCTCTGGGAAGGAG
  CAAGTTGGCATAAAGATTAATGGCCAAACACAATCTGTTGTATTTTCATACAAGGGACTGGATGGAAGTC
  TCCAAACAGCAGCCTTTTCGAATTTGTCCTCCTTGTTTGATTCCCAGTGGCATAAGATCATGATTGGCGT
  GGAGAGGAGTAGTGCTACTCTTTTTGTTGACTGCAACAGGATTGAATCTTTACCTATAAAGCCAAGAGGC
  CCAATTGACATTGATGGCTTTGCTGTGCTGGGAAAACTTGCAGATAATCCTCAAGTTTCTGTTCCATTTG
  AACTTCAATGGATGCTGATCCATTGTGACCCCCTGCGGCCCAGGAGAGAAACTTGCCATGAGCTGCCAGC
  CAGAATAACGCCCAGCCAGACCACCGACGAGAGAGGTCCCCCGGGTGAGCAGGGTCCTCCCGGGCCTCCG
  GGCCCCCCTGGAGTTCCAGGCATCGATGGCATCGACGGTGACCGAGGTCCTAAGGGCCCCCCGGGCCCCC
  CGGGTCCTGCAGGTGAACCGGGAAAGCCAGGAGCTCCAGGCAAGCCTGGCACACCTGGCGCTGATGGATT
  AACAGGACCTGATGGATCCCCTGGCTCCATTGGGTCAAAGGGACAAAAAGGAGAACCTGGTGTGCCTGGA
  TCGCGTGGATTTCCAGGCCGTGGTATTCCTGGACCCCCTGGTCCTCCTGGGACAGCAGGACTCCCTGGAG
  AGCTTGGCCGTGTAGGACCTGTTGGTGACCCTGGGAGAAGAGGACCACCTGGCCCCCCTGGCCCCCCAGG
  ACCCAGAGGAACAATTGGCTTTCATGATGGAGATCCATTGTGTCCCAATGCCTGTCCACCAGGTCGCTCA
  GGATATCCAGGCCTACCAGGCATGAGGGGTCATAAAGGGGCTAAAGGAGAAATTGGTGAACCAGGAAGAC
  AAGGACACAAGGGTGAAGAAGGTGACCAGGGAGAACTCGGAGAAGTTGGAGCTCAAGGACCTCCAGGAGC
  CCAGGGTTTGCGAGGCATCACCGGCATAGTTGGGGACAAAGGGGAAAAAGGTGCTCGGGGCTTAGATGGT
  GAACCTGGGCCTCAGGGTCTTCCTGGTGCACCTGGTGATCAAGGACAGCGAGGACCTCCAGGAGAAGCAG
  GTCCCAAAGGAGATAGAGGGGCTGAAGGTGCTAGAGGAATTCCTGGTCTCCCTGGGCCCAAAGGAGACAC
  GGGTTTGCCAGGTGTGGATGGCCGTGATGGGATCCCTGGAATGCCTGGAACAAAGGGTGAACCAGGAAAA
  CCTGGGCCTCCTGGTGATGCAGGATTGCAGGGGTTACCAGGTGTACCTGGAATTCCTGGTGCAAAGGGTG
  TTGCTGGTGAAAAGGGTAGCACAGGTGCTCCAGGGAAGCCTGGTCAGATGGGAAATTCAGGCAAACCGGG
  CCAACAGGGGCCTCCAGGAGAGGTGGGACCCCGAGGACCCCAGGGGCTTCCTGGCAGTAGAGGAGAATTA
  GGACCAGTGGGATCCCCAGGCCTACCAGGTAAACTGGGTTCTCTGGGTAGCCCTGGCCTCCCTGGCTTGC
  CTGGGCCCCCTGGACTTCCTGGAATGAAAGGTGACAGGGGTGTAGTCGGTGAACCGGGTCCAAAGGGTGA
  ACAGGGTGCCTCTGGTGAAGAAGGTGAAGCAGGAGAAAGGGGGGAACTTGGAGATATAGGATTACCTGGC
  CCAAAGGGATCTGCAGGTAATCCTGGGGAACCTGGCTTGAGAGGGCCTGAGGGAAGTCGGGGGCTTCCTG
  GAGTGGAAGGACCAAGAGGACCACCTGGACCCCGGGGTGTGCAGGGAGAACAGGGTGCCACCGGCCTGCC
  TGGTGTCCAGGGCCCTCCGGGTAGAGCACCGACAGATCAGCACATTAAGCAGGTTTGCATGAGAGTCATA
  CAAGAACATTTTGCTGAGATGGCTGCCAGTCTTAAGCGTCCAGACTCAGGTGCCACTGGGCTTCCTGGAA
  GGCCTGGCCCTCCTGGTCCCCCCGGCCCTCCTGGAGAGAATGGTTTCCCAGGCCAGATGGGAATTCGTGG
  CCTTCCGGGCATTAAGGGGCCCCCTGGTGCTCTTGGTTTGAGGGGACCTAAAGGTGACTTGGGAGAAAAG
  GGGGAGCGTGGCCCTCCAGGAAGAGGTCCCAACGGTTTGCCTGGAGCTATAGGTCTCCCAGGTGACCCAG
  GCCCTGCCAGCTATGGCAGAAATGGCCGAGACGGTGAGCGAGGCCCCCCAGGGGTGGCAGGAATTCCTGG
  AGTGCCTGGACCCCCGGGACCTCCTGGGCTTCCCGGTTTCTGTGAGCCAGCCTCCTGCACCATGCAGGCT
  GGTCAGCGAGCATTTAACAAAGGGCCTGACCCTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001851
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001851.4, NP_001842.3
• RefSeq Size: 3805 bp
• RefSeq ORF: 2766 bp
• Locus ID: 1297
• Cytogenetics: 6q13
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1, also known as the long form).