Collagen XV (COL15A1) (NM_001855) Human Untagged Clone

CAT#: SC303085

COL15A1 (untagged)-Human collagen, type XV, alpha 1 (COL15A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL15A1
• Synonyms: FLJ38566
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001855, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCACCAAGGAGGAACAACGGGCAGTGCTGGTGTCTGCTGATGCTGCTCTCGGTCTCCACGCCCCTCC
  CTGCTGTCACCCAGACCCGCGGTGCGACAGAGACTGCTTCCCAGGGTCACCTGGACCTCACGCAGCTCAT
  CGGTGTCCCGCTGCCCTCGTCCGTATCCTTTGTCACAGGCTATGGTGGCTTCCCGGCCTACAGTTTCGGG
  CCTGGTGCCAATGTTGGCCGCCCAGCCAGGACTCTCATCCCATCCACCTTCTTCAGGGACTTCGCCATCA
  GCGTCGTGGTGAAGCCCAGCAGCACCCGTGGTGGCGTGCTCTTCGCCATCACTGACGCCTTCCAGAAGGT
  CATCTACCTGGGCCTGCGGCTCTCAGGTGTGGAGGACGGCCACCAGCGGATCATCCTCTACTACACGGAG
  CCAGGCTCCCATGTGTCCCAAGAGGCTGCTGCCTTCTCGGTGCCTGTGATGACCCACAGGTGGAACCGCT
  TCGCCATGATTGTCCAGGGTGAGGAAGTGACCCTCCTCGTGAACTGTGAGGAGCACAGCCGCATCCCCTT
  CCAGCGGTCCTCCCAGGCTTTGGCTTTTGAGTCCAGCGCTGGAATCTTCATGGGCAATGCAGGAGCTACA
  GGGCTCGAGAGATTCACTGGCTCCCTCCAGCAGCTCACCGTGCACCCCGACCCCAGGACTCCCGAGGAGC
  TGTGTGACCCTGAAGAGTCCTCGGCATCTGGAGAGACCAGTGGGCTGCAGGAGGCAGACGGAGTAGCTGA
  GATCTTAGAAGCCGTCACCTACACTCAAGCCTCGCCCAAAGAAGCAAAAGTTGAACCCATAAACACACCT
  CCAACTCCATCCTCCCCCTTTGAAGACATGGAACTTTCTGGTGAACCTGTACCCGAGGGGACCCTGGAAA
  CCACCAACATGAGCATCATCCAGCACAGCAGCCCCAAACAAGGGTCTGGTGAGATCCTGAATGACACACT
  GGAGGGGGTTCATTCTGTGGATGGTGACCCCATTACTGACAGCGGCTCAGGGGCTGGGGCCTTCCTTGAC
  ATTGCTGAAGAAAAGAATTTAGCAGCAACAGCAGCGGGGCTGGCCGAGGTGCCCATCAGCACTGCTGGAG
  AAGCAGAGGCCAGCAGTGTGCCCACCGGGGGACCAACCCTCTCTATGTCCACGGAGAACCCAGAGGAAGG
  GGTCACTCCAGGTCCAGATAATGAAGAGCGTTTAGCAGCAACAGCAGCAGGGGAGGCCGAGGCACTCGCC
  AGCATGCCTGGGGAAGTGGAGGCCAGTGGTGTGGCCCCCGGGGAGCTGGACCTCTCCATGTCCGCCCAGA
  GCCTCGGGGAAGAGGCCACTGTGGGTCCAAGCAGTGAAGACAGTTTAACAACAGCTGCAGCTGCAACCGA
  AGTGTCCCTCAGTACTTTTGAGGATGAGGAAGCCAGTGGGGTCCCCACAGATGGCCTGGCTCCCCTCACA
  GCCACCATGGCCCCTGAGCGGGCAGTCACTTCTGGTCCTGGTGATGAAGAAGACTTGGCAGCAGCCACAA
  CAGAGGAGCCCCTCATCACAGCTGGGGGTGAAGAGTCCGGCAGCCCTCCCCCTGATGGGCCACCGCTGCC
  CCTGCCCACAGTGGCTCCTGAAAGATGGATCACTCCAGCTCAAAGAGAACATGTGGGAATGAAAGGACAG
  GCTGGGCCCAAAGGAGAAAAGGGTGATGCTGGGGAGGAGCTTCCTGGCCCTCCTGAACCTTCTGGGCCTG
  TTGGACCCACGGCAGGAGCAGAAGCAGAGGGCTCTGGCCTAGGCTGGGGCTCGGACGTCGGCTCTGGCTC
  TGGTGACCTGGTGGGCAGTGAGCAGCTGCTGAGAGGTCCTCCAGGACCCCCAGGGCCACCTGGCTTACCT
  GGGATTCCAGGAAAACCAGGAACTGATGTTTTCATGGGACCCCCTGGATCTCCTGGAGAGGATGGACCTG
  CTGGTGAACCTGGGCCCCCGGGCCCTGAGGGACAGCCTGGAGTTGATGGAGCCACCGGCCTTCCCGGGAT
  GAAAGGGGAGAAGGGAGCAAGAGGGCCTAATGGCTCAGTTGGTGAAAAGGGTGACCCTGGCAACAGAGGC
  TTACCTGGACCCCCGGGGAAAAAGGGACAAGCTGGCCCTCCTGGGGTCATGGGACCCCCAGGGCCTCCTG
  GACCCCCTGGGCCCCCAGGCCCTGGATGCACAATGGGACTTGGATTCGAGGATACCGAAGGCTCTGGAAG
  CACCCAGCTATTGAATGAACCCAAACTCTCCAGACCAACGGCTGCAATTGGTCTCAAAGGAGAGAAAGGA
  GACCGGGGACCCAAGGGAGAAAGGGGGATGGATGGAGCCAGTATTGTGGGACCCCCTGGGCCGAGAGGGC
  CACCTGGGCACATCAAGGTCTTGTCTAATTCCTTGATCAATATCACCCATGGATTCATGAATTTCTCGGA
  CATTCCTGAGCTGGTGGGGCCTCCGGGGCCGGACGGGTTGCCTGGGCTGCCAGGATTTCCAGGTCCTAGA
  GGACCAAAAGGTGACACTGGTTTACCTGGCTTTCCAGGACTAAAAGGAGAACAGGGCGAGAAGGGAGAGC
  CGGGTGCCATCCTGACAGAGGACATTCCTCTGGAAAGGCTGATGGGGAAAAAGGGTGAACCTGGAATGCA
  TGGAGCCCCAGGACCAATGGGGCCCAAAGGACCACCAGGACATAAAGGAGAATTTGGCCTTCCCGGGCGA
  CCTGGTCGCCCAGGACTGAATGGCCTCAAGGGTACCAAAGGAGATCCAGGGGTCATTATGCAGGGCCCAC
  CTGGCTTACCTGGCCCTCCAGGCCCCCCTGGGCCACCTGGAGCTGTGATTAACATCAAAGGAGCCATTTT
  CCCAATACCCGTCCGACCACACTGCAAAATGCCAGTTGATACTGCTCATCCTGGGAGTCCAGAGCTCATC
  ACTTTTCACGGTGTTAAAGGAGAGAAAGGATCCTGGGGTCTTCCTGGCTCAAAGGGAGAAAAAGGCGACC
  AGGGAGCCCAGGGACCACCAGGTCCTCCACTTGATCTAGCTTACCTGAGACACTTTCTGAACAACTTGAA
  GGGGGAGAATGGAGACAAGGGGTTCAAAGGTGAAAAAGGAGAAAAAGGAGACATTAATGGCAGCTTCCTT
  ATGTCTGGGCCTCCAGGCCTGCCCGGAAATCCAGGCCCGGCTGGCCAAAAAGGGGAGACAGTCGTTGGGC
  CCCAAGGACCCCCAGGTGCTCCTGGTCTGCCTGGGCCACCTGGCTTTGGAAGACCTGGTGATCCTGGGCC
  ACCGGGGCCCCCGGGGCCACCAGGACCTCCAGCTATCCTGGGAGCAGCTGTGGCCCTTCCAGGTCCCCCT
  GGCCCTCCAGGACAGCCAGGGCTTCCCGGATCCAGAAACCTGGTCACAGCATTCAGCAACATGGATGACA
  TGCTGCAGAAAGCGCATTTGGTTATAGAAGGAACATTCATCTACCTGAGGGACAGCACTGAGTTTTTCAT
  TCGTGTTAGAGATGGCTGGAAAAAATTACAGCTGGGAGAACTGATCCCCATTCCTGCCGACAGCCCTCCA
  CCCCCTGCGCTTTCCAGCAACCCACATCAGCTTCTGCCTCCACCAAACCCTATTTCAAGTGCCAATTATG
  AGAAGCCTGCTCTGCATTTGGCTGCTCTGAACATGCCATTTTCTGGGGACATTCGAGCTGATTTTCAGTG
  CTTCAAGCAGGCCAGAGCTGCAGGACTGTTGTCCACCTACCGAGCATTCTTATCTTCCCATTTGCAAGAT
  CTGTCCACCATTGTGAGGAAAGCAGAGAGATACAGCCTTCCCATAGTGAACCTCAAGGGCCAAGTACTTT
  TTAATAATTGGGACTCAATTTTTTCTGGCCACGGAGGTCAGTTCAATATGCATATTCCAATATACTCCTT
  TGATGGTCGAGACATAATGACAGATCCTTCTTGGCCCCAGAAAGTCATTTGGCATGGCTCCAGCCCCCAT
  GGCGTCCGCCTTGTGGATAACTACTGTGAAGCATGGCGAACCGCGGACACAGCGGTCACGGGACTTGCCT
  CCCCGCTGAGCACGGGGAAGATTCTGGACCAGAAAGCATACAGCTGTGCTAATCGGCTAATTGTCCTATG
  TATCGAAAACAGTTTCATGACAGACGCTAGGAAGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001855
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001855.4, NP_001846.3
• RefSeq Size: 5422 bp
• RefSeq ORF: 4167 bp
• Locus ID: 1306
• Cytogenetics: 9q22.33
• Gene Summary: 'This gene encodes the alpha chain of type XV collagen, a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Type XV collagen has a wide tissue distribution but the strongest expression is localized to basement membrane zones so it may function to adhere basement membranes to underlying connective tissue stroma. The proteolytically produced C-terminal fragment of type XV collagen is restin, a potentially antiangiogenic protein that is closely related to endostatin. Mouse studies have shown that collagen XV deficiency is associated with muscle and microvessel deterioration. [provided by RefSeq, May 2013]'