MSH4 (NM_002440) Human Untagged Clone

CAT#: SC303201

MSH4 (untagged)-Human mutS homolog 4 (E. coli) (MSH4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSH4
• Synonyms: mutS homolog 4; mutS homolog 4 (E. coli); OTTHUMP00000011349
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002440, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGAGGCCTGAGATCTCATCAACCTCGCCTTCTGCCCCGGCGGTTTCCCCGTCGTCGGGAGAAACCC
  GCTCACCTCAGGGTCCCCGCTACAATTTCGGACTCCAGGAGACTCCACAGAGCCGCCCTTCGGTCCAGGT
  GGTCTCTGCATCCACCTGTCCTGGCACGTCAGGAGCTGCGGGCGACCGGAGCAGCAGCAGCAGCAGCCTT
  CCCTGCCCCGCGCCAAACTCCCGGCCAGCTCAAGGTTCATACTTTGGAAACAAAAGAGCTTATGCAGAAA
  ACACAGTTGCATCAAATTTTACTTTTGGTGCAAGCTCATCTTCTGCACGAGATACTAATTATCCTCAAAC
  ACTTAAAACTCCATTGTCTACTGGAAATCCTCAGAGATCAGGTTATAAGAGCTGGACACCACAAGTGGGA
  TATTCAGCTTCATCCTCATCTGCGATTTCTGCACACTCCCCATCAGTTATTGTAGCTGTTGTAGAAGGGA
  GAGGACTTGCCAGAGGTGAAATAGGAATGGCAAGTATTGATTTAAAAAACCCCCAAATTATACTATCCCA
  GTTTGCAGACAACACAACATATGCAAAGGTGATCACTAAACTTAAAATTTTATCACCTTTGGAAATAATA
  ATGTCAAATACTGCTTGTGCTGTGGGGAATTCCACCAAGTTGTTCACTCTGATCACAGAAAATTTCAAGA
  ATGTTAATTTCACTACTATCCAAAGGAAATACTTCAATGAAACAAAAGGATTAGAGTACATTGAACAGTT
  ATGCATAGCAGAATTCAGCACTGTCCTAATGGAGGTTCAGTCCAAGTATTACTGCCTTGCAGCTGTTGCA
  GCTTTGTTAAAATATGTTGAATTTATTCAAAATTCAGTTTATGCACCAAAATCACTGAAGATTTGTTTCC
  AGGGTAGTGAACAGACAGCCATGATAGATTCATCATCAGCCCAAAACCTTGAATTGTTAATTAATAATCA
  AGACTATAGGAATAATCACACTCTCTTTGGTGTTCTAAATTATACTAAGACTCCTGGAGGGAGTAGACGA
  CTTCGTTCTAATATATTAGAGCCTCTAGTTGATATTGAAACCATTAACATGAGATTAGATTGTGTTCAAG
  AACTACTTCAAGATGAGGAACTATTTTTTGGACTTCAATCAGTTATATCAAGATTTCTTGATACAGAGCA
  GCTTCTTTCTGTTTTAGTCCAAATTCCAAAGCAAGACACGGTCAATGCTGCTGAATCAAAGATAACAAAT
  TTAATATACTTAAAACATACCTTGGAACTTGTGGATCCTTTAAAGATTGCTATGAAGAACTGTAACACAC
  CTTTATTAAGAGCTTACTATGGTTCCTTGGAAGACAAGAGGTTTGGAATCATACTTGAAAAGATTAAAAC
  AGTAATTAATGATGATGCAAGATACATGAAAGGATGCCTAAACATGAGGACTCAGAAGTGCTATGCAGTG
  AGGTCTAACATAAATGAATTTCTTGACATAGCAAGAAGAACATACACAGAGATTGTAGATGACATAGCAG
  GAATGATATCACAACTTGGAGAAAAATATAGTCTACCTTTAAGGACAAGTTTTAGCTCTGCTCGAGGATT
  TTTCATCCAGATGACTACAGATTGTATAGCCCTACCTAGTGATCAACTTCCTTCAGAATTTATTAAGATT
  TCTAAAGTGAAAAATTCTTACAGCTTTACATCAGCAGATTTAATTAAAATGAATGAAAGATGCCAAGAAT
  CTTTGAGAGAAATCTATCACATGACTTATATGATAGTGTGCAAACTGCTTAGTGAGATTTATGAACATAT
  TCATTGCTTATATAAACTATCTGACACTGTGTCAATGCTGGATATGCTACTGTCATTTGCTCATGCCTGC
  ACTCTTTCTGACTATGTTCGACCAGAATTTACTGATACTTTAGCAATCAAACAGGGATGGCATCCTATTC
  TTGAAAAAATATCTGCGGAAAAACCTATTGCCAACAATACCTATGTTACAGAAGGGAGTAATTTTTTGAT
  CATAACTGGACCAAACATGAGTGGAAAATCCACATATTTAAAACAGATTGCTCTTTGTCAGATTATGGCC
  CAGATTGGATCATATGTTCCAGCAGAATATTCTTCCTTTAGAATTGCTAAACAGATTTTTACAAGAATTA
  GTACTGATGATGATATCGAAACAAATTCATCAACATTTATGAAAGAAATGAAAGAGATAGCATATATTCT
  ACATAATGCTAATGACAAATCGCTCATATTAATTGATGAACTTGGCAGAGGTACTAATACGGAAGAAGGT
  ATTGGCATTTGTTATGCTGTTTGTGAATATCTACTGAGCTTAAAGGCATTTACACTGTTTGCTACACATT
  TCCTGGAACTATGCCATATTGATGCCCTGTATCCTAATGTAGAAAACATGCATTTTGAAGTTCAACATGT
  AAAGAATACCTCAAGAAATAAAGAAGCAATTTTGTATACCTACAAACTTTCTAAGGGACTCACAGAAGAG
  AAAAATTATGGATTAAAAGCTGCAGAGGTGTCATCACTTCCACCATCAATTGTCTTGGATGCCAAGGAAA
  TCACAACTCAAATTACGAGACAAATTTTGCAAAACCAAAGGAGTACCCCTGAGATGGAAAGACAGAGAGC
  TGTGTACCATCTAGCCACTAGGCTTGTTCAAACTGCTCGAAACTCTCAATTGGATCCAGACAGTTTACGA
  ATATATTTAAGTAACCTCAAGAAGAAGTACAAAGAAGATTTTCCCAGGACTGAACAAGTTCCAGAAAAGA
  CTGAAGAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002440
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002440.3, NP_002431.2
• RefSeq Size: 3293 bp
• RefSeq ORF: 2811 bp
• Locus ID: 4438
• Cytogenetics: 1p31.1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction, but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. This protein and MSH5 form a heterodimer which binds uniquely to a Holliday Junction and its developmental progenitor, thus provoking ADP-ATP exchange, and stabilizing the interaction between parental chromosomes during meiosis double-stranded break repair. [provided by RefSeq, Aug 2011]'