PDE7A (NM_002603) Human Untagged Clone

CAT#: SC303225

PDE7A (untagged)-Human phosphodiesterase 7A (PDE7A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE7A
• Synonyms: HCP1; PDE7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002603 edited
  TTGGAAGGCAGCTGCTTGAGGTCCAAGGCAGTCAGTGTCCCCTCTCTTTTGCCTCGGGAC
  AGCTGGTATTTATCAGACTCCTAAGAAGTTTTCCTTGCTCCCTAGTAGAAGAGAGAGATT
  ATGCAGCGGGCTTTTGATTGATCCAATGGGAATTACATTGATCTGGTGTCTGGCCTTGGT
  TCTTATCAAGTGGATCACCTCTAAGAGGCGTGGAGCTATTTCCTATGACAGTTCTGATCA
  GACTGCATTATACATTCGTATGCTAGGAGATGTACGTGTAAGGAGCCGAGCAGGATTTGA
  ATCAGAAAGAAGAGGTTCTCACCCATATATTGATTTTCGTATTTTCCACTCTCAATCTGA
  AATTGAAGTGTCTGTCTCTGCAAGGAATATCAGAAGGCTACTAAGTTTCCAGCGATATCT
  TAGATCTTCACGCTTTTTTCGTGGTACTGCGGTTTCAAATTCCCTAAACATTTTAGATGA
  TGATTATAATGGACAAGCCAAGTGTATGCTGGAAAAAGTTGGAAATTGGAATTTTGATAT
  CTTTCTATTTGATAGACTAACAAATGGAAATAGTCTAGTAAGCTTAACCTTTCATTTATT
  TAGTCTTCATGGATTAATTGAGTACTTCCATTTAGATATGATGAAACTTCGTAGATTTTT
  AGTTATGATTCAAGAAGATTACCACAGTCAAAATCCTTACCATAACGCAGTCCACGCTGC
  GGATGTTACTCAGGCCATGCACTGTTACTTAAAGGAACCTAAGCTTGCCAATTCTGTAAC
  TCCTTGGGATATCTTGCTGAGCTTAATTGCAGCTGCCACTCATGATCTGGATCATCCAGG
  TGTTAATCAACCTTTCCTTATTAAAACTAACCATTACTTGGCAACTTTATACAAGAATAC
  CTCAGTACTGGAAAATCACCACTGGAGATCTGCAGTGGGCTTATTGAGAGAATCAGGCTT
  ATTCTCACATCTGCCATTAGAAAGCAGGCAACAAATGGAGACACAGATAGGTGCTCTGAT
  ACTAGCCACAGACATCAGTCGCCAGAATGAGTATCTGTCTTTGTTTAGGTCCCATTTGGA
  TAGAGGTGATTTATGCCTAGAAGACACCAGACACAGACATTTGGTTTTACAGATGGCTTT
  GAAATGTGCTGATATTTGTAACCCATGTCGGACGTGGGAATTAAGCAAGCAGTGGAGTGA
  AAAAGTAACGGAGGAATTCTTCCATCAAGGAGATATAGAAAAAAAATATCATTTGGGTGT
  GAGTCCACTTTGCGATCGTCACACTGAATCTATTGCCAACATCCAGATTGGTTTTATGAC
  TTACCTAGTGGAGCCTTTATTTACAGAATGGGCCAGGTTTTCCAATACAAGGCTATCCCA
  GACAATGCTTGGACACGTGGGGCTGAATAAAGCCAGCTGGAAGGGACTGCAGAGAGAACA
  GTCGAGCAGTGAGGACACTGATGCTGCATTTGAGTTGAACTCACAGTTATTACCTCAGGA
  AAATCGGTTATCATAACCCCCAGAACCAGTGGGACAAACTGCCTCCTGGAGGTTTTTAGA
  AATGTGAAATGGGGTCTTGAGGTGAGAGAACTTAACTCTTGACTGCCAAGGTTTCCAAGT
  GAGTGATGCCAGCCAGCATTATTTATTTCCAAGATTTCCTCTGTTGGATCATTTGAACCC
  ACTTGTTAATTGCAAGACCCGAACATACAGCAATATGAATTTGGCTTT
  
• Restriction Sites: Please inquire
• ACCN: NM_002603
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_002603.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002603.1, NP_002594.1
• RefSeq Size: 1739 bp
• RefSeq ORF: 1371 bp
• Locus ID: 5150
• Cytogenetics: 8q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (1, also called PDE7A2) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (a) has a distinct N-terminus compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.