RFNG (NM_002917) Human Untagged Clone

CAT#: SC303249

RFNG (untagged)-Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFNG
• Synonyms: beta-1,3-N-acetylglucosaminyltransferase radical fringe; radical fringe homolog; RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002917 edited
  ATGAGCCGCGCGCGTGGGGCGCTGTGCCGGGCCTGCCTCGCGCTGGCCGCGGCCCTGGCC
  GCGCTGCTGTTACTGCCGCTGCCGCTGCCCCGCGCGCCCGCCCCGGCCCGGACCCCCGCC
  CCGGCCCCGCGCGCGCCCCCGTCCCGGCCCGCTGCCCCCAGCCTGCGGCCTGACGACGTC
  TTCATCGCCGTCAAGACCACCCGGAAGAACCACGGGCCGCGCCTGCGGCTGCTGCTGCGC
  ACCTGGATCTCCCGGGCCCGCCAGCAGACGTTTATCTTCACCGACGGGGACGACCCTGAG
  CTCGAGCTCCAGGGCGGCGACCGTGTCATCAACACCAACTGCTCGGCGGTGCGCACTCGT
  CAGGCCCTCTGCTGCAAGATGTCCGTGGAGTATGACAAGTTCATTGAGTCCGGGCGCAAG
  TGGTTTTGCCACGTGGATGATGACAATTATGTGAACGCAAGGAGCCTCCTGCACCTGCTC
  TCCAGCTTCTCACCCAGCCAGGACGTCTACCTGGGGCGGCCCAGCCTGGACCACCCCATT
  GAGGCCACCGAGAGGGTCCAGGGTGGCAGAACTGTGACCACGGTCAAGTTCTGGTTTGCT
  ACTGGTGGGGCCGGGTTCTGCCTCAGCAGAGGCCTTGCCCTCAAGATGAGCCCATGGGCC
  AGCCTGGGCAGCTTCATGAGCACAGCTGAGCAGGTGCGGCTGCCGGATGACTGCACAGTT
  GGCTACATCGTGGAGGGGCTCCTGGGCGCCCGCCTGCTGCACAGCCCCCTCTTCCACTCT
  CACCTGGAGAACCTGCAGAGGCTGCCGCCCGACACCCTGCTCCAGCAGGTTACCTTGAGC
  CATGGGGGTCCTGAGAACCCACAGAACGTGGTGAACGTGGCTGGAGGCTTCAGCCTGCAT
  CAAGACCCCACACGGTTTAAGTCTATCCATTGTCTTCTGTACCCAGACACGGACTGGTGT
  CCCAGGCAGAAACAGGGCGCCCCGACCTCTCGGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002917 unedited
  GACCTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCACCAGGTCCCGGCGGCCG
  CATGAGCCGCGCGCGTGGGGCGCTGTGCCGGGCCTGCCTCGCGCTGGCCGCGGCCCTGGC
  CGCGCTGCTGTTACTGCCGCTGCCGCTGCCCCGCGCGCCCGCCCCGGCCCGGACCCCCGC
  CCCGGCCCCGCGCGCGCCCCCGTCCCGGCCCGCTGCCCCCAGCCTGCGGCCTGACGACGT
  CTTCATCGCCGTCAAGACCACCCGGAAGAACCACGGGCCGCGCCTGCGGCTGCTGCTGCG
  CACCTGGATCTCCCGGGCCCGCCAGCAGACGTTTATCTTCACCGACGGGGACGACCCTGA
  GCTCGAGCTCCAGGGCGGCGACCGTGTCATCAACACCAACTGCTCGGCGGTGCGCACTCG
  TCAGGCCCTCTGCTGCAAGATGTCCGTGGAGTATGACAAGTTCATTGAGTCCGGGCGCAA
  GTGGTTTTGCCACGTGGATGATGACAATTATGTGAACGCAAGGAGCCTCCTGCACCTGCT
  CTCCAGCTTCTCACCCAGCCAGGACGTCTACCTGGGGCGGCCCACCCTGGACCACCCCAT
  TGAGGCCACCGAGAGGGTCCAGGGTGGCAGAACTGTGACCACGGTCAAGTTCTGGTTTGC
  TACTGGTGGGGCCGGCTTCTGCCTCAGCAGAGGCCTTGCCCTCAAGATGAGCCAATGGGC
  TAGCCTGAGCAGATTCATGAGCACAGCTGAACAAGTGCGGCTGCCGGATGACCTGCCCAG
  CTGGCTACTCGAGGACGGGCCTCCATGGGACGCCCAGCTGCTGCACAGCACCCTCTTCCA
  GTCTCCACTGGCAGAATCGTGGCAAAGCGTGCTCGTCAGACAGCAGGATCGGATAGCTTA
  CCTGAAGCCAGGTGGGGGTGCTGGAGATACCACAGACAGCGATGAATAGAGATTAGGAGG
  CATAGA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_002917 unedited
  CGCTTTAGTCTATCCTTGTCTTCTGTACCCAGGCACGGACTGGTGTCCCATGCAGAAACA
  GGGCGCCCCGACCTCTCGGTGACACCAACCACCCCGACCCAGGGCTGCCTGGCTCTGTCC
  CAGGCGCGGGGAACCAGAGCCCCCTATGGGCTCAGTGGGCTCCCTCAGGTGCCACGGCCG
  CACCAGTGAGATGCAGGCACCTGGCAGACCCTCTGGCTAGCCTGCAGCCCCCCCTCTCCC
  AGCCCCTGGTGGGCTGTGGTGATGGGTGTTTTGGGAGAACGAAGACAGCCAGGCTGATGG
  CCAGGGCCGCAGTGCCCCTCCCCCCGACCCAGCCCCAAGGTTGATCCCACGGGAACAGGC
  TTCCACCCCAGCACTTGCGCACCTGGGAGGGAGCTGCCATCCGGGCTCCATTACCTGTTG
  CTGAAGGCGGGTGCTAGGCTGGCTGGGTGTCAAGGAGCAGGCTCCAGGCCAAGGTCCTGG
  CCCAGCCACGGCCATTGCAAGGGCTCAGCCTGGCAGGCTTTGTGGGGGACGCCGCCCTCT
  CTGCCGCAGGCTGGGTGCACGGCCAGGCACCACAGTGGGACTCAGCCCGGGAAGGTCATG
  TTCTCGACCAGAGCTTTGCTCCCAGTCCAGGCGCCTCATTCGGAGGCCTCTTGACTGGGA
  CCACAGAGATGTTTTCTCCGCTCTGACTTGTGGCTCAGGACTACTTTCTGGGTCGTGCTC
  CTGCCCCCACTGTGCCTGGGCCCCATAAACAAACAGGAGCCTTTTGTTCCGCTGACCTGC
  CCATCCCAGCAGAACCCACCTCCCCGCCTGGTGCCTTTCCATGGAAGGGAAATGGGAACA
  GGGCCGTCATCTCCCCTCTGCCTCCTGCTTTGCTGTTGCCCGAGCACGAGTAAAGCTTTT
  GTTTCTTAACTTCACAGAAAACGACAGATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002917
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002917.1, NP_002908.1
• RefSeq Size: 1828 bp
• RefSeq ORF: 996 bp
• Locus ID: 5986
• Cytogenetics: 17q25.3
• Protein Families: Transmembrane
• Protein Pathways: Notch signaling pathway
• Gene Summary: ''